LOCUS       sequence-218347-       10860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control 
            expression vector (positive control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti PA-mCit-NL
FEATURES             Location/Qualifiers
     source          1..10860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    138..255
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        320..1531
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1368..1387
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1569..1742
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1743..1916
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             2258..2269
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    2640..2664
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2667..2676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2673..3185
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     protein_bind    complement(3187..3211)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3250..3838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3303..3323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3721..3732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3841..3857)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3842..3858)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4363..4543
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4569..4586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4575..4799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4845..5273
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4932..4951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5142..5163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5282..5302)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5287..5616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5467..5602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5529..5548
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5664..5711
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5730..6104
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6234..6355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6271..6290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6325..6344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6404..6420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6404..6420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6417..6439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6428..6444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6452..6458,6459..6476,6477..6482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6497..6518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6635..6652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6806..7394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6886..6905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7565..8356,8357..8425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8188..8207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8426..8530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8605..8624)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8796..9175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9177..9375
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9330..9350
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9393..9573
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9620..9745
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10238..10471
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10656..10700
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
       61 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
      121 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      181 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      241 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttaattaac
      301 ccgtgtcggc tccagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
      361 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc
      421 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
      481 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
      541 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
      601 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
      661 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
      721 agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
      781 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
      841 ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg
      901 cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa
      961 cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg
     1021 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     1081 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     1141 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     1201 ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     1261 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     1321 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     1381 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
     1441 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
     1501 attctggccg tttttggctt ttttgttaga cgaagcttgg gcccgggaat taattcgcta
     1561 gcaccatggt ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac
     1621 atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc
     1681 caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caggcgccga
     1741 aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc ttacatttac
     1801 ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat gacccaagcc
     1861 aaagcgctaa ccttttagca gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag
     1921 acgcgaactc ggaattcggc tcgggctcgg tatcgaaagg tgaagagctg tttactggcg
     1981 tcgtgcccat tttggtggaa cttgacggtg acgtgaacgg tcacaaattc tcagtctcgg
     2041 gagaaggcga gggggacgcg acatatggga aactcacact gaagttcatc tgcacgactg
     2101 gaaagctgcc tgtcccttgg cctacactcg tcacgacgtt cggttatggt ttgatgtgtt
     2161 tcgcaagata tcctgaccac atgaaacagc atgatttctt taagagcgcc atgcccgaag
     2221 gttatgtcca ggagaggact atcttcttta aggacgatgg gaactacaag actcgggcag
     2281 aggtgaaatt tgagggggac acgcttgtga atagaatcga actcaaaggc atcgatttca
     2341 aggaggatgg aaacattctc ggacacaaac ttgagtacaa ttacaacagc cacaacgtgt
     2401 atatcatggc ggacaaacag aagaatggaa tcaaagtgaa cttcaagatc agacacaata
     2461 tcgaagatgg gtcagtgcag ctggctgatc attaccagca gaatacgccc atcggggacg
     2521 gtccagtctt gttgccggat aaccactacc tttcatacca gtcgaagttg tccaaagacc
     2581 ccaacgagaa gagggatcac atggtgcttc ttgaatttgt cacagccgct ggtaagctta
     2641 caagtttgta caaaaaagca ggcttcgcca ccatggtctt cacactcgaa gatttcgttg
     2701 gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt
     2761 ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg
     2821 gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg
     2881 accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact
     2941 ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg
     3001 actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa
     3061 cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct
     3121 ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc
     3181 tggcggaccc agctttcttg tacaaagtgg tgtaactcga gtctagaatt cgatatcaag
     3241 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3301 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3361 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3421 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3481 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3541 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3601 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3661 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3721 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3781 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3841 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     3901 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     3961 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4021 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     4081 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     4141 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     4201 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     4261 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     4321 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     4381 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4441 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4501 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     4561 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4621 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4681 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4741 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4801 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4861 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4921 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4981 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5041 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5101 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5161 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5221 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5281 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5341 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5401 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5461 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5521 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5581 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5641 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     5701 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     5761 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     5821 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     5881 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     5941 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     6001 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     6061 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     6121 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6181 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6361 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6421 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6481 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6541 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8581 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8641 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8701 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8761 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     8821 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     8881 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     8941 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9001 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     9061 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     9121 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     9181 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     9241 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     9301 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     9361 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9421 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9481 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9541 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     9601 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     9661 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     9721 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     9781 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     9841 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     9901 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     9961 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    10021 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    10081 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    10141 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10201 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10261 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10321 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10381 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    10441 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    10501 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    10561 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    10621 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    10681 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    10741 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    10801 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
//
