LOCUS       sequence-218351-       11992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal 
            fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti PA-mCit-GW
FEATURES             Location/Qualifiers
     source          1..11992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        224..1435
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1272..1291
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1473..1646
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1647..1820
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             2162..2173
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    2544..2668
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2693..2698,2699..2716,2717..2723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2777..3457
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2843..2862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3777..4082
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3961..3980
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4123..4247)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4286..4874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4339..4359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4757..4768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4877..4893)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4878..4894)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5399..5579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5605..5622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5611..5835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5881..6309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5968..5987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6178..6199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6318..6338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6323..6652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6503..6638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6565..6584
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6700..6747
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6766..7140
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7270..7391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7307..7326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7361..7380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7440..7456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7440..7456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7453..7475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7464..7480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7488..7494,7495..7512,7513..7518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7671..7688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7922..7941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8601..9392,9393..9461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9224..9243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9462..9566)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9641..9660)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9832..10211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10213..10411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10366..10386
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10429..10609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10656..10781
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11274..11507
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11692..11736
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
       61 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      121 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      181 agagatccag tttggttaat taacccgtgt cggctccaga tctggcctcc gcgccgggtt
      241 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
      301 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
      361 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
      421 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
      481 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
      541 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
      601 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
      661 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
      721 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
      781 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
      841 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
      901 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
      961 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     1021 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     1081 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1141 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1201 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     1261 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     1321 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     1381 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc
     1441 ttgggcccgg gaattaattc gctagcacca tggtggacaa caaattcaac aaagaacaac
     1501 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
     1561 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
     1621 agctaaatga tgctcaggcg ccgaaagtag acaacaaatt caacaaagaa caacaaaacg
     1681 cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc
     1741 aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa
     1801 atggtgctca ggcgccgaaa gtagacgcga actcggaatt cggctcgggc tcggtatcga
     1861 aaggtgaaga gctgtttact ggcgtcgtgc ccattttggt ggaacttgac ggtgacgtga
     1921 acggtcacaa attctcagtc tcgggagaag gcgaggggga cgcgacatat gggaaactca
     1981 cactgaagtt catctgcacg actggaaagc tgcctgtccc ttggcctaca ctcgtcacga
     2041 cgttcggtta tggtttgatg tgtttcgcaa gatatcctga ccacatgaaa cagcatgatt
     2101 tctttaagag cgccatgccc gaaggttatg tccaggagag gactatcttc tttaaggacg
     2161 atgggaacta caagactcgg gcagaggtga aatttgaggg ggacacgctt gtgaatagaa
     2221 tcgaactcaa aggcatcgat ttcaaggagg atggaaacat tctcggacac aaacttgagt
     2281 acaattacaa cagccacaac gtgtatatca tggcggacaa acagaagaat ggaatcaaag
     2341 tgaacttcaa gatcagacac aatatcgaag atgggtcagt gcagctggct gatcattacc
     2401 agcagaatac gcccatcggg gacggtccag tcttgttgcc ggataaccac tacctttcat
     2461 accagtcgaa gttgtccaaa gaccccaacg agaagaggga tcacatggtg cttcttgaat
     2521 ttgtcacagc cgctggtaag cttacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2581 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2641 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     2701 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     2761 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2821 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2881 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2941 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     3001 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     3061 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     3121 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     3181 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     3241 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     3301 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     3361 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     3421 gcaggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca gtatgcgtat
     3481 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3541 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3601 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3661 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3721 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     3781 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     3841 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     3901 tgtcagataa agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     3961 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     4021 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     4081 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     4141 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4201 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgta actcgagtct
     4261 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4321 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4381 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4441 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4501 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4561 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4621 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4681 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4741 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4801 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4861 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     4921 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     4981 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5101 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5161 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5221 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     5281 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     5341 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     5401 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5461 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5521 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5581 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5761 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5821 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5881 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5941 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6001 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6061 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6121 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6181 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6241 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6301 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6661 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     6721 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     6781 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     6841 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     6901 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     6961 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7021 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7081 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7141 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7201 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7261 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7381 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7501 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9601 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9661 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9721 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     9781 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     9841 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10021 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10201 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
    10261 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    10321 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    10381 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
    10441 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10501 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10561 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10621 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    10681 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    10741 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
    10801 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
    10861 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    10921 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    10981 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    11041 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    11101 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    11161 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    11221 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    11281 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    11341 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    11401 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    11461 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    11521 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    11581 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    11641 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    11701 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    11761 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    11821 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    11881 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    11941 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gc
//
