LOCUS       sequence-218353-       11986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal 
            fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti GW-mCit-PA
FEATURES             Location/Qualifiers
     source          1..11986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    149..266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        331..1542
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1379..1398
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1574..1698
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1723..1728,1729..1746,1747..1753)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1807..2487
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1873..1892)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2807..3112
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2991..3010
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3153..3277)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3593..3604
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3987..4160
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             4161..4334
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     misc_feature    4387..4975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4440..4460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4858..4869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4978..4994)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4979..4995)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5500..5680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5706..5723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5712..5936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5982..6410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6069..6088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6279..6300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6419..6439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6424..6753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6604..6739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6666..6685
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6801..6848
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6867..7241
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7371..7492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7408..7427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7462..7481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7541..7557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7541..7557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7554..7576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7565..7581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7589..7595,7596..7613,7614..7619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7634..7655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7772..7789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7943..8531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8023..8042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8702..9493,9494..9562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9325..9344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9563..9667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9742..9761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9933..10312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10314..10512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10467..10487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10530..10710
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10757..10882
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11375..11608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11793..11837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
       61 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
      121 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      181 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      241 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
      301 ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg
      361 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
      421 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
      481 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
      541 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
      601 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
      661 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
      721 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
      781 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
      841 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
      901 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
      961 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     1021 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     1081 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     1141 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     1201 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     1261 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     1321 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     1381 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     1441 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     1501 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg ggcccgggaa
     1561 ttaattcgct agcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     1621 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     1681 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     1741 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     1801 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     1861 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     1921 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     1981 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2041 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2101 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2161 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2221 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2281 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2341 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2401 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggcgggg
     2461 cgtaatctag aggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     2521 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     2581 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     2641 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     2701 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     2761 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     2821 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     2881 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     2941 agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     3001 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     3061 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     3121 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     3181 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     3241 tttacgtttc tcgttcagct ttcttgtaca aagtggtgaa gcttgtatcg aaaggtgaag
     3301 agctgtttac tggcgtcgtg cccattttgg tggaacttga cggtgacgtg aacggtcaca
     3361 aattctcagt ctcgggagaa ggcgaggggg acgcgacata tgggaaactc acactgaagt
     3421 tcatctgcac gactggaaag ctgcctgtcc cttggcctac actcgtcacg acgttcggtt
     3481 atggtttgat gtgtttcgca agatatcctg accacatgaa acagcatgat ttctttaaga
     3541 gcgccatgcc cgaaggttat gtccaggaga ggactatctt ctttaaggac gatgggaact
     3601 acaagactcg ggcagaggtg aaatttgagg gggacacgct tgtgaataga atcgaactca
     3661 aaggcatcga tttcaaggag gatggaaaca ttctcggaca caaacttgag tacaattaca
     3721 acagccacaa cgtgtatatc atggcggaca aacagaagaa tggaatcaaa gtgaacttca
     3781 agatcagaca caatatcgaa gatgggtcag tgcagctggc tgatcattac cagcagaata
     3841 cgcccatcgg ggacggtcca gtcttgttgc cggataacca ctacctttca taccagtcga
     3901 agttgtccaa agaccccaac gagaagaggg atcacatggt gcttcttgaa tttgtcacag
     3961 ccgctggtga attcggctcg ggctcggtgg acaacaaatt caacaaagaa caacaaaacg
     4021 cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc
     4081 aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa
     4141 atgatgctca ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa aacgcgttct
     4201 atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt
     4261 taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatggtg
     4321 ctcaggcgcc gaaagtagac gcgaactcgt gactcgagtc tagaattcga tatcaagctt
     4381 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4441 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4501 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4561 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4621 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4681 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4741 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4801 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4861 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4921 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4981 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     5041 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     5101 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5161 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     5221 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     5281 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     5341 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     5401 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     5461 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     5521 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5581 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5641 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     5701 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5761 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5821 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5881 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5941 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6001 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6061 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6121 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6181 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6241 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6301 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6361 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6421 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6481 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6541 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6601 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6661 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6721 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6781 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     6841 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     6901 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     6961 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     7021 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     7081 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     7141 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     7201 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     7261 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7321 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7501 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9661 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9721 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9781 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9841 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9901 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     9961 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    10021 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    10081 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    10141 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    10201 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10261 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    10321 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    10381 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    10441 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    10501 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10681 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10741 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10801 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10861 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10921 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    10981 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    11041 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    11101 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    11161 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    11221 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    11281 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    11341 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    11401 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    11461 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    11521 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    11581 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    11641 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    11701 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    11761 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    11821 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    11881 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    11941 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagac
//
