LOCUS       sequence-218375-        2552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, RecJ is a ssDNA specific exonuclease, 
            involved in genome repair and maintenance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-recJD
FEATURES             Location/Qualifiers
     source          1..2552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(500..517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(671..1259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(751..770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1430..2221,2222..2290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2053..2072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2291..2395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2463..2481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2485..2528)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2531..2550
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gaagcatcgc agaaatgaac atgctgtctc cttttggcat gctgaacccg
       61 aaaccgcacg ttctcgtaga aaatgcagta ctggaagatg tccgcaagat aggggcaaat
      121 aaaactcatg tcaaaatgac gatcagaaac gagtcgtccc agcttgattg cgtcggtttt
      181 aataaaggcg agcttcaaga aggcatcgta ccgggttcaa gaatttccat tgtcggtgag
      241 atgtcaatca acgaatggaa caatcgaaaa aagccgcagc ttatgataaa ggatgccgct
      301 gtttctgaat ggcagctgtt tgatttaagg ggaaaacgga cgtgggaaga cacagtttcc
      361 gcccttccat ctgctaaacg ggccattgtc tctttcaaag aagactcaac aacattactt
      421 caaactgaag accttcgccg agaagtgcac gtcatcagtt ctaaagatca agcaaagtta
      481 gagagacgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      661 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1081 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1561 tgataccgcg agagccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2461 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2521 atgatttctg gaattcgcgg ccgcttctag aa
//
