LOCUS       sequence-218378-        2545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, rrnO-23S (23S rRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-rrnOD
FEATURES             Location/Qualifiers
     source          1..2545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(493..510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(664..1252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(744..763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1423..2214,2215..2283))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2046..2065
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2284..2388)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2456..2474
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2478..2521)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2524..2543
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa ggcgttctaa gggctgtgaa gccagaccgg aaggactggt ggagcgctta
       61 gaagtgagaa tgccggtatg agtagcgaaa gaggggtgag aatcccctcc accgaatgcc
      121 taaggtttcc tgaggaaggc tcgtccgctc agggttagtc gggacctaag ccgaggccga
      181 aaggcgtagg cgatggacaa caggttgata ttcctgtacc acctcctcac catttgagca
      241 atggggggac gcaggaggat agggtaagcg cggtattgga tatccgcgtc caagcagtta
      301 ggctgggaaa taggcaaatc cgtttcccat aaggctgagc tgtgatggcg agcgaaatat
      361 agtagcgaag ttcctgattc cacactgcca agaaaagcct ctagcgaggt gagaggtgcc
      421 cgtaccgcaa accgacacag gtaggcgagg agagaatcct aaggtgatcg ttagagagac
      481 gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      661 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      841 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1081 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1561 gcgagagcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2401 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2461 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     2521 ctggaattcg cggccgcttc tagaa
//
