LOCUS       sequence-218383-        2509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, SpsC is involved in spore coat 
            polysaccharide synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-spsCD
FEATURES             Location/Qualifiers
     source          1..2509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(457..474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(628..1216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(708..727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1387..2178,2179..2247))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2010..2029
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2248..2352)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2420..2438
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2442..2485)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2488..2507
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gagatgaata tgtttgattt gcaggcggcc ctcggacttc atcagctgaa
       61 acggctggat gatatgcaga aacggagaga agagattgcc ggccgctatc aaaccgcttt
      121 tcagcagatt cctgggctga tcacgccgtt tgtccatgat gacggcaggc acgcctggca
      181 tctatatgtt ctgcaagttg atgaaaaaaa ggccggggtt acgcgcagcg aaatgattac
      241 ggctttaaag gatgaataca atatcggcac tagcgttcat tttattccag tacacattca
      301 tccgtattat caaaagcaat ttggatataa ggaagcggac tttccgaatg ccatgaatta
      361 ctataaacga accctttctc tcccgctcta tccatcaatg tctgacgatg atgtagatga
      421 tgtcattgaa gctgttagag agacgctgca ggcttcctcg ctcactgact cgctgcgctc
      481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      601 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1321 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1381 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1441 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1501 tggccccagt gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc
     1561 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1621 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1681 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1741 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1801 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1861 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1921 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1981 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2041 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2101 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2161 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2221 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2281 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2341 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2401 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     2461 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagaa
//
