LOCUS       sequence-218385-        2633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, Pair of HAs spans yckA and yckB, which share 
            homology with ABC transporters in B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-yckABU
FEATURES             Location/Qualifiers
     source          1..2633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(581..598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(752..1340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(832..851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1511..2302,2303..2371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2134..2153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2372..2476)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2544..2562
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2566..2609)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2612..2631
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tggaaaatcg tagtcgctac atcaggtacg ctctatccca cttcttatca
       61 cgatactgac tctggctctg ataagctgac gggctacgag gttgaagttg ttcgtgaagc
      121 ggcaaaacgc ttggggctga aggttgagtt caaggagatg ggcattgacg ggatgctgac
      181 agcggtgaac agcggacagg ttgacgcagc cgcgaatgac atcgatgtga cgaaggaccg
      241 tgaggagaag ttcgcatttt ccactccata taaatattca tacggcacgg caattgtccg
      301 aaaagacgat ttatcaggga ttaaaacgct caaggattta aaagggaaga aagcggctgg
      361 cgcggctaca accgtctaca tggaggtcgc gagaaaatac ggcgccaaag aagtgattta
      421 tgataatgcg acgaatgagc aatatttaaa agatgttgcc aacggacgca ctgacgtcat
      481 tttaaacgac tattacctgc agacacttgc gctcgcggca ttcccggatc tcaacattac
      541 aattcatcct gacatcaatg ccagagacgc tgcaggcttc ctcgctcact gactcgctgc
      601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
      781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1621 catctggccc cagtgctgca atgataccgc gagagccacg ctcaccggct ccagatttat
     1681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2521 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     2581 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gaa
//
