LOCUS       sequence-218386-        2654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, Pair of HAs spans yckA and yckB, which 
            share homology with ABC transporters in B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-yckABD
FEATURES             Location/Qualifiers
     source          1..2654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(602..619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(773..1361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(853..872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1532..2323,2324..2392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2155..2174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2393..2497)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2565..2583
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2587..2630)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2633..2652
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gccattgaat cgttccctta tgtcataaaa ggcattggtt atacactgct
       61 catctctttt gtaagcatgt ttgcgggaac tgtgatcggc ctgttcatat cactcgccag
      121 aatgtctaaa cttgctttgt taagatggcc ggcgaagctc tatatctcat ttatgcgagg
      181 cgttccgatt ttggtgattt tgtttatcct ttatttcggg tttccttata tcggcattga
      241 attttccgct gtcacggctg ctttaatcgg ctttagccta aacagcgccg cctacattgc
      301 ggaaattaac cgttcagcca tttcatccgt cgaaaaagga cagtgggaag ccgcatcctc
      361 gctgggtctt tcctattggc agacgatgag gggcatcatt ttgccgcaat cgatacgtat
      421 tgcgctgcct ccgcttgcca atgtattgct cgacttgatc aaggcgtcct ctctcgctgc
      481 gatgattaca gtgccggaat tgctccagca tgcgaaaatc atcgggggca gagagtttga
      541 ttatatgacc atgtatattc tgaccgctct catttattgt tagagagacg ctgcaggctt
      601 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      661 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      721 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
      781 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      841 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      901 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      961 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1021 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1081 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1141 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1201 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1261 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1321 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1381 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1441 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1501 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1561 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1621 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac
     1681 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1741 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1801 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1861 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1921 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1981 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2041 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2101 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2161 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2221 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2281 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2341 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2401 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2461 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2521 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2581 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     2641 ggccgcttct agaa
//
