LOCUS       sequence-218393-        2937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloramphenicol resistance cassette (expressing chloramphenicol 
            acetyltransferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-C-cat
FEATURES             Location/Qualifiers
     source          1..2937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(885..902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1056..1644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1136..1155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1815..2606,2607..2675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2438..2457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2676..2780)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2848..2866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2870..2913)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2916..2935
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaccg gcacccatta gttcaacaaa cgaaaattgg ataaagtggg atatttttaa
       61 aatatatatt tatgttacaa taatattgac ttttaaaaaa ggattgattc taatgaagaa
      121 agcagacaag taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg
      181 aactttaata aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg
      241 aaccaacaaa cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac
      301 ataaaacaag aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata
      361 aactcaaata cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat
      421 aagttagagc cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg
      481 actcctgtaa agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa
      541 tataatggtt cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt
      601 tctattattc catggacttc atttactggg tttaacttaa atatcaataa taatagtaat
      661 taccttctac ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta
      721 ccgctatctt tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg
      781 aactctattc aggaattgtc agataggcct aatgactggc ttttataata tgagataatg
      841 ccgactgtac tttttacagt cggaagagag acgctgcagg cttcctcgct cactgactcg
      901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1921 ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc ggctccagat
     1981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2821 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2881 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagaa
//
