LOCUS       sequence-218419-        9033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VCPIP1 with C-terminal strep-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Hulsmann J, Kravic B, Weith M, Gstaiger M, Aebersold RH, Collins BC,
            Meyer H
  TITLE     AP-SWATH reveals direct involvement of VCP/p97 in integrated stress 
            response signaling through facilitating CReP/PPP1R15B degradation.
  JOURNAL   Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 
            10.1074/mcp.RA117.000471.
  PUBMED    29599191
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 
            10.1074/mcp.RA117.000471."
COMMENT     Sequence Label:  pcDNA5FRT/TO-VCPIP1-Strep-HA
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        532..735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     686..706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    737..755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    758..776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     786..810
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    953..977
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4647..4671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4718..4741
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4778..4801
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4832..4858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4832..4858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4902..4919)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4908..5132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5265..5284)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5416..5463
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5471..6493
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5471..6493
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6623..6744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6660..6679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6714..6733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6793..6809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6793..6809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6806..6828)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6817..6833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6841..6847,6848..6865,6866..6871))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6886..6907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7024..7041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7195..7783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7275..7294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7954..8745,8746..8814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8577..8596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8815..8919)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8994..9013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
      781 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      841 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt
      901 ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt
      961 gtacaaaaaa gttggcatgt ctcagccgcc gccgccgccg cctccgttgc cgccgccacc
     1021 tcctccccct gaggctccac agactccgtc gtccttggcg tcggcggctg cttcgggggg
     1081 gcttttgaag cggagagacc ggagaatcct ttccgggagc tgcccggatc cgaagtgtca
     1141 ggcgcgtcta tttttcccgg cctccggttc tgtcagcatc gagtgtaccg agtgcggcca
     1201 gcggcacgag cagcaacagc tgctgggggt tgaggaggtg accgacccgg acgtagtgct
     1261 acacaacctg ctgcggaacg cgctgctcgg ggttacgggg gcacccaaga agaacacgga
     1321 actggtaaag gtgatgggcc tttccaacta tcactgcaaa ttgttgtcgc ccatattagc
     1381 tcgctatgga atggacaaac agacaggccg ggccaagctt ctccgggaca tgaaccaggg
     1441 cgaactgttc gattgcgcct tactgggtga ccgcgccttc ctcatagaac cagagcatgt
     1501 taacactgtg ggctatggca aggaccgctc cggaagcctc ctgtatttgc atgacactct
     1561 ggaggacatt aagcgggcca ataaaagcca ggaatgtctc attccagtgc atgtggacgg
     1621 ggatggacac tgcttggtgc atgctgtgtc tcgggctcta gtaggccgag agctcttctg
     1681 gcatgcctta agagagaatc ttaaacagca ctttcagcag cacctggccc gatatcaagc
     1741 tctgttccat gacttcattg atgctgctga gtgggaggac attatcaatg agtgtgaccc
     1801 tctgtttgta ccacctgagg gtgttccctt gggcctgagg aatatccaca tatttggtct
     1861 tgccaatgtg ctacatcgtc ctattattct gttagattcc ctcagtggca tgagaagctc
     1921 tggtgattat tcagccacct ttctacctgg gctcatccct gcagagaagt gcactgggaa
     1981 agatggtcat ttgaacaaac caatctgtat tgcatggagc agctccggta gaaaccatta
     2041 tatccccttg gtaggcataa aaggggctgc tttgcccaaa ctgcctatga atttgcttcc
     2101 taaagcatgg ggtgtgcctc aggaccttat taaaaagtac ataaaacttg aagaggatgg
     2161 tggttgtgtt attggaggtg acagaagttt gcaagataaa tacttactta ggcttgttgc
     2221 tgctatggaa gaagtcttta tggacaaaca tggtatccat cctagtttgg ttgctgatgt
     2281 ccatcagtat ttctacagaa ggactggagt gataggagtt cagcctgagg aagtcacagc
     2341 agctgctaaa aaagcagtaa tggataatcg ccttcacaaa tgtttgctct gtggtgccct
     2401 ttctgaactt catgttcctc cagagtggtt ggctcctgga gggaaattgt ataacctggc
     2461 aaaaagtact catggacagc tgaggactga caaaaattac agctttccct tgaacaattt
     2521 ggtttgctca tatgattcag tgaaagatgt tctggtacca gactatggaa tgagtaacct
     2581 aacagcttgt aattggtgcc atggcacatc tgtgcgaaag gtcagaggag atgggtctat
     2641 tgtgtatttg gatggagaca gaactaattc taggtccact ggtggcaaat gtggttgtgg
     2701 attcaaacac ttttgggatg gtaaggagta tgacaatcta ccagaagctt tccctattac
     2761 tttagaatgg ggtggaagag tggtcagaga aacagtatat tggttccagt atgaaagtga
     2821 ttcatctttg aatagtaatg tttacgatgt tgcaatgaaa cttgttacca agcactttcc
     2881 aggtgaattt gggagtgaaa tcctagttca gaaagttgtc cacactatat tgcatcagac
     2941 tgccaaaaag aatcccgatg attatactcc tgtaaatata gatggtgctc acgcccaaag
     3001 agttggagat gttcaaggac aagaatcaga gtctcagctc ccaactaaaa ttattcttac
     3061 tggacagaaa acaaaaactt tgcacaagga ggagttaaac atgagtaaaa ctgaaagaac
     3121 tattcaacag aatattacgg aacaggcttc tgtaatgcag aaacggaaaa cagagaagtt
     3181 aaaacaagaa caaaaagggc aacccaggac tgtttctccc agtaccattc gtgatggtcc
     3241 atcctctgca cctgctacac ctaccaaggc tccctattca ccgacaactt ctaaggagaa
     3301 gaagatccga atcacaacta atgatggacg acagtccatg gttaccctta agtcttcaac
     3361 aacctttttt gaacttcagg aaagtatagc cagagaattc aacattcctc catatttaca
     3421 gtgtattcga tacgggtttc ctcctaaaga gttaatgcca ccacaggcag gaatggaaaa
     3481 ggaaccagtt cctttacagc atggcgacag aattacaata gaaattctaa aaagtaaagc
     3541 tgaaggtggt cagtctgctg cagcacactc agcccacact gtgaaacaag aagatattgc
     3601 tgttactggt aaactgtcat ctaaggaact tcaggagcaa gctgaaaaag aaatgtactc
     3661 cttgtgtctt ttagcaacat taatgggaga agatgtatgg tcttatgcaa agggacttcc
     3721 tcacatgttt cagcagggtg gtgtattcta cagtattatg aagaaaacca tgggtatggc
     3781 tgatggcaag cattgtactt ttccacatct gcctggcaaa acctttgtct ataatgcttc
     3841 tgaagataga ctggaattgt gtgtggatgc tgcaggacat ttccccattg gtcctgatgt
     3901 tgaagattta gttaaagagg ctgtaagtca ggttcgagca gaggctacta caagaagtag
     3961 ggaatcaagt ccctcacatg ggctattaaa actaggtagt ggtggagtag tgaaaaagaa
     4021 atctgagcaa cttcataacg taactgcctt tcagggaaaa gggcattctt taggaactgc
     4081 atctggtaac ccacaccttg atccaagagc tagggaaact tcagttgtaa gaaagcataa
     4141 tacagggaca gactttagta atagttccac taaaacagag ccttctgtat tcacagcttc
     4201 ttctagtaat agtgagctta ttcgaatagc tcctggagta gtaacaatga gagacggcag
     4261 gcagcttgat cctgatttgg ttgaggccca gcgaaaaaaa ttgcaggaaa tggtttcttc
     4321 tattcaggct tcaatggaca ggcaccttcg ggatcaaagt acagagcagt caccatctga
     4381 tcttcctcaa aggaaaacag aagttgtgag ttcttctgca aagtctggga gtcttcagac
     4441 tggtttgcct gaatcttttc ctttaactgg tggtactgaa aatttgaata cagaaacaac
     4501 tgatggctgt gtagcagatg cactgggagc agcctttgcc acaaggtcaa aagcacaaag
     4561 gggaaattcc gtggaggagc ttgaagagat ggatagtcaa gatgctgaga tgactaacac
     4621 aactgagcca atggatcact cttgctaccc aactttcttg tacaaagtgg ttgatatcaa
     4681 ggccgccgac atcaccagcc tgtacaagaa agtggggtgg agccatcccc agttcgagaa
     4741 gggcggagga tctggcggcg gaagcggcgg aggaagctgg tcccaccctc agtttgagaa
     4801 aggcaccgag ctgggcagca ccatggccag ctacccctac gacgtgcccg actacgcctg
     4861 atgactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4921 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4981 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5041 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5101 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5161 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5221 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5281 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5341 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5401 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     5461 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     5521 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5581 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5641 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5701 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     5761 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     5821 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     5881 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     5941 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     6001 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     6061 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     6121 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6181 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     6241 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6301 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6361 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6421 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6481 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     6541 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6601 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6721 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6781 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8821 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8881 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8941 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9001 tgctctgatg ccgcatagtt aagccagtat ctg
//
