LOCUS       sequence-21848-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Shoichet SA, Baumer AT, Stamenkovic D, Sauer H, Pfeiffer AF, Kahn 
            CR, Muller-Wieland D, Richter C, Ristow M
  TITLE     Frataxin promotes antioxidant defense in a thiol-dependent manner 
            resulting in diminished malignant transformation in vitro.
  JOURNAL   Hum Mol Genet. 2002 Apr 1. 11(7):815-21.
  PUBMED    11929854
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2002 Apr 1. 11(7):815-21."
COMMENT     Sequence Label: pBlue-hFX-HA
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4..20
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(61..87)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(731..747)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(731..747)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(779..798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(808..825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(808..824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctcgaggtc gacggtatcg ataagcttga tatcgaattc tagatgtatc ggtacgtgtc
       61 tgcgtagtct ggtacgtcgt aagggtaagc atcttttccg gaataggcca aggaagacaa
      121 gtccagtttg gtttttaagg ctttagtgag ctctgcggcc agcagctcat ggagggacac
      181 gccgtcgtgg gagtacaccc agtttttccc agtccagtca taacgcttag gtccactgga
      241 tggagaagat agccagattt gcttgtttgg cgtctgcttg ttgatcacat aggttcctag
      301 atctccaccc agtttgacag ttaagacacc actcccaaag gagacatcat agtcctcaaa
      361 cgtgtatggc ttgtctgcaa ggtcttcaaa aaactctgct aaagagtcca gcgtttcctc
      421 tgctagtctt tcataggtgg tctcatctag agagcctggg tggcccaaag ttccagattt
      481 cctcaaattc atcaaataga cactctgctt tttgacattc caaatctggt tgaggccacg
      541 ttggttcgaa cttgcgcggc ggggcgtgca ggtcgcatcg atgtcggtgc gcaggccacg
      601 gcggccgcag agtggggcca actctgccgg ccgcgggacc cgggtgaggg tctgggcctg
      661 ggccgggctg ggtgacgcca ggaggccggc tactgcgcgg cgcccgagag tccacatgct
      721 gctccggcgg gatccactag ttctagagcg gccgccaccg cggtggagct ccaattcgcc
      781 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
      841 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagct
//
