LOCUS       sequence-21849-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Dhe-Paganon S, Shigeta R, Chi YI, Ristow M, Shoelson SE
  TITLE     Crystal structure of human frataxin.
  JOURNAL   J Biol Chem. 2000 Oct 6. 275(40):30753-6.
  PUBMED    10900192
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Oct 6. 275(40):30753-6."
COMMENT     Sequence Label: pET28(a)-hFX-HA
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(109..135)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(514..546)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(550..567)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(577..594)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             613..635
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    654..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctttcgngct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcta gatgtatcgg tacgtgtctg cgtagtctgg
      121 tacgtcgtaa gggtaagcat cttttccgga ataggccaag gaagacaagt ccagtttggt
      181 ttttaaggct ttagtgagct ctgcggccag cagctcatgg agggacacgc cgtcgtggga
      241 gtacacccag tttttcccag tccagtcata acgcttaggt ccactggatg gagaagatag
      301 ccagatttgc ttgtttggcg tctgcttgtt gatcacatag gttcctagat ctccacccag
      361 tttgacagtt aagacaccac tcccaaagga gacatcatag tcctcaaacg tgtatggctt
      421 gtctgcaagg tcttcaaaaa actctgctaa agagtccagc gtttcctctg ctagtctttc
      481 ataggtggtc tcatctagag agccggatcc gcgacccatt tgctgtccac cagtcatgct
      541 agccatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat gatggctgct
      601 gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      661 ccgctcacaa ttnnnnatag tgagtcgtat taatttcgcg ggatcgagat ctcga
//
