LOCUS       sequence-21850-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Ristow lab plasmids
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28(a)-hFX(I154F)-HA
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(114..140)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(519..551)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(555..572)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(582..599)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             618..640
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    655..679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(679..698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(680..698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gntnnctcct tcgggcttnt ngcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgcaa gcttgtcgac ggagctcgaa ttctagatgt atcggtacgt gtctgcgtag
      121 tctggtacgt cgtaagggta agcatctttt ccggaatagg ccaaggaaga caagtccagt
      181 ttggttttta aggctttagt gagctctgcg gccagcagct catggaggga cacgccgtcg
      241 tgggagtaca cccagttttt cccagtccag tcataacgct taggtccact ggatggagaa
      301 gatagccaga attgcttgtt tggcgtctgc ttgttgatca cataggttcc tagatctcca
      361 cccagtttga cagttaagac accactccca aaggagacat catagtcctc aaacgtgtat
      421 ggcttgtctg caaggtcttc aaaaaactct gctaaagagt ccagcgtttc ctctgctagt
      481 ctttcatagg tggtctcatc tagagagccg gatccgcgac ccatttgctg tccaccagtc
      541 atgctagcca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg atgatgatgg
      601 ctgctgccca tggtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
      661 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      721 atcctctacg ccggacgcat cgtgncngca tcacggcgca caggtgcggt gctggcgcta
      781 tatcgcaaac nccgannggn aaacggccnc ctncgctcag accctnttcg ctggtaggng
      841 cagccctgcc gggattggcc atccttgngc catctggggg g
//
