LOCUS       sequence-21853-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Ristow lab plasmids
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBlue-hFX(I154F)(AA88)-HA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(63..89)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(460..476)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..476)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(508..527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(509..527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(537..554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(537..553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(546..568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(782..801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tctagatgta tcggtacgtg
       61 tctgcgtagt ctggtacgtc gtaagggtaa gcatcttttc cggaataggc caaggaagac
      121 aagtccagtt tggtttttaa ggctttagtg agctctgcgg ccagcagctc atggagggac
      181 acgccgtcgt gggagtacac ccagtttttc ccagtccagt cataacgctt aggtccactg
      241 gatggagaag atagccagaa ttgcttgttt ggcgtctgct tgttgatcac ataggttcct
      301 agatctccac ccagtttgac agttaagaca ccactcccaa aggagacatc atagtcctca
      361 aacgtgtatg gcttgtctgc aaggtcttca aaaaactctg ctaaagagtc cagcgtttcc
      421 tctgctagtc tttcataggt ggtctcatct agagagccgg atccactagt tctagagcgg
      481 ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      661 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcag
//
