LOCUS       sequence-218555-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal adhesion kinase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-FAK-5
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        89..392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        393..596
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     547..567
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      635..644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             641..1348
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(786..804)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1054..1073
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4680..4801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4717..4736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4771..4790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4808..5263)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4945..4966)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5157..5176
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5290..5394
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5396..5753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5396..5587
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5419..5439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5604..5739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5788..6582
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5788..6582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5842..5861)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6452..6471
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6770..6789)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6814..6861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7190..7778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7679..7698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
       61 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      121 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      181 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      241 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      301 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      361 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      421 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      481 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      541 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
      601 tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga
      661 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      721 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      781 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      841 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      901 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      961 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     1021 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     1081 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     1141 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     1201 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     1261 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     1321 caccggcggc atggacgagc tgtacaagtc cggactcaga tctatggcag cagcttacct
     1381 tgatccaaac ttgaatcata caccaagttc aagtgcaaag acgcacctcg gtactgggat
     1441 ggagcgttcc ccgggggcca tggagcgagt cctaaaggtt tttcactact ttgaaaacag
     1501 cagcgagcca acgacgtggg ccagcattat ccggcatgga gatgctactg atgttcgagg
     1561 cataatacag aagattgtgg actgtcacaa agtgaaaaat gtggcctgct atgggttgcg
     1621 actcagtcat ctgcagtctg aggaggttca ctggctgcac ctggacatgg gggtatccaa
     1681 tgtgagagag aaatttgaac tagcacatcc tccagaagaa tggaaatatg aactgagaat
     1741 tcggtacctg cccaaaggat ttctaaacca gttcactgag gacaaaccaa ctttaaattt
     1801 tttctatcag caggtgaaaa atgactatat gttagaaata gcagatcaag tggaccagga
     1861 aattgctttg aaactaggtt gccttgaaat caggagatcc tacggagaga tgagaggcaa
     1921 tgcattagag aagaaatcca actatgaagt gctagaaaaa gatgtcggtt taagacgatt
     1981 ttttccgaag agtttgctag attcagtgaa ggccaaaaca ctacgaaaat taatccaaca
     2041 gacatttcga caatttgcca acctcaacag agaagaaagt attttgaaat tctttgagat
     2101 cctctctcca gtgtacagat ttgacaagga atgcttcaag tgtgcccttg gttcaagctg
     2161 gattatttca gtggagctgg caattggccc agaggaagga atcagctacc ttacagacaa
     2221 gggtgcaaat ccaactcacc tggcagattt taatcaagta caaactattc agtattcaaa
     2281 cagtgaagac aaggacagaa aagggatgtt gcaactgaag atagctggtg cacctgagcc
     2341 tctgacagtg acagcaccat ccttaaccat tgcagagaat atggctgact tgatagacgg
     2401 atactgccga ctggtgaatg gagccacgca atcttttatt atcaggccac agaaagaagg
     2461 tgaaagagct ttaccatcaa taccaaagct ggccaacaat gagaagcaag gagtaaggtc
     2521 gcacacagtc tctgtatcag aaacagatga ctatgcagag ataatagatg aagaagatac
     2581 ttatacaatg ccatcaacca gagattatga aattcaaagg gagagaattg aactggggcg
     2641 ctgcattggt gaaggacagt ttggagatgt gcaccaagga atttacatga gtccggaaaa
     2701 tccagctatg gctgtagcaa tcaaaacatg taaaaactgc acctcagaca gcgttagaga
     2761 aaagttccta caagaagcct taacaatgcg tcagtttgat catcctcaca ttgtgaagct
     2821 cattggagtt attacagaaa acccagtgtg gataatcatg gagctctgta cacttggaga
     2881 gttgagatcg tttctgcaag taagaaaatt cagcttggac ctggcctccc tcatcctcta
     2941 cgcttaccag cttagcacag cacttgctta cctagagagc aaaagatttg tacatagaga
     3001 tattgctgct aggaacgtgc tggtatctgc cactgactgt gtgaaattgg gtgactttgg
     3061 cttatcccga tacatggaag acagtactta ctataaagct tccaaaggaa agttacctat
     3121 caaatggatg gctccagagt caatcaactt ccgacggttt acctcagcaa gcgatgtgtg
     3181 gatgtttggt gtgtgtatgt gggagatcct gatgcatggg gtaaagccct tccagggagt
     3241 gaaaaataat gatgttattg gtcggattga gaacggtgag cggctcccca tgcctccgaa
     3301 ctgccctccc accctctaca gccttatgac caagtgctgg gcatacgacc ctagtagacg
     3361 acccaggttt actgaactta aagcacaact cagtacaata ctggaggagg agaagctgca
     3421 gcaagaggaa cgaatgagaa tggaatccag gcgacaagtc acagtatcct gggactcagg
     3481 aggatcagat gaagctcctc ccaagcccag caggcctggt taccccagcc caaggtccag
     3541 tgaagggttt tatccgagtc ctcagcatat ggtacagcca aatcactacc aggtatctgg
     3601 ctactctggt tctcatggga taccagccat ggcaggcagc atttatcctg ggcaagcttc
     3661 tctcttggat caaacagatt cctggaacca tcgacctcag gaagtatcag catggcagcc
     3721 aaacatggag gattcgggca ctttggatgt acgaggaatg gggcaggttc tgcccacaca
     3781 tctcatggag gagaggttaa taagacaaca gcaagagatg gaagaagatc aacgctggct
     3841 tgagaaagag gaacgattcc tggtaatgaa acctgatgtg cggctctcca gaggcagcat
     3901 tgaacgggag gacggaggtc tccagggccc agctggtaac cagcacatat atcagcctgt
     3961 gggtaaacca gatcatgccg ctccaccaaa gaagccccct cgccctggag ccccccactt
     4021 gggcagcctc gcgagcctga acagccccgt ggacagctac aacgaaggcg tgaagatcaa
     4081 gccacaggaa atcagccctc ctcctacggc caacctggac cgctccaatg acaaagtcta
     4141 tgagaatgta accgggctgg tgaaagctgt catagagatg tccagtaaaa tacagccagc
     4201 tccgccagag gagtacgtgc ccatggtaaa ggaggttggc ttggcgctga gaaccttgct
     4261 agcaacagtg gatgagtcgc tgccagtgct tcctgcaagc acccacagag agattgagat
     4321 ggcccagaaa ctgctgaact ctgacctggc tgagctcatt aacaagatga agctggccca
     4381 gcagtacgtc atgaccagcc tgcagcagga gtacaagaag caaatgctga cggctgctca
     4441 cgctctggct gtggatgcca agaacttgct ggatgtcatc gatcaagcca gactgaaaat
     4501 gatcagccag tccaggccca ctaagctgcc gtagccgaat tctgcagatg ggatccaccg
     4561 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4621 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4801 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4861 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4921 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4981 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5041 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5101 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5161 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5221 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     5281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     5401 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     5761 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5821 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5881 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5941 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     6001 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6061 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6121 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6181 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6241 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6301 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6361 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6421 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6481 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6541 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6601 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6661 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6721 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     6781 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     6841 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     6901 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     6961 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     7021 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7861 attc
//
