LOCUS       sequence-218574-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cry1 transcription luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE
  TITLE     Long-term in vivo recording of circadian rhythms in brains of freely
            moving mice.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 
            10.1073/pnas.1717735115.
  PUBMED    29610316
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 
            10.1073/pnas.1717735115."
COMMENT     Sequence Label: AAV-P(Cry1)-DIO-intron336-dLUC
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    complement(1725..1758)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1809..1842)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(1852..1971)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(1975..2022)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             complement(2029..3675)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             complement(3685..3705)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(3730..3774)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(3796..3861)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(4252..4275)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4275..4284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4322..4340)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4323..4340)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(4346..4364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4407..4426)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4409..4541
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    4601..4634
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4685..4718
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    4737..5325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4790..4810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5208..5219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5357..5833
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5556..5575)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5873..6013
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5873..6002
                     /label=AAV2 ITR
     rep_origin      6088..6543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6175..6194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6385..6406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6560..6579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6679..6701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6739..6757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6825..6929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6930..6998,6999..7790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7148..7167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7961..8549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8450..8469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtaaag atgcacatgt ggccctgtgt tctcattaga
      181 cttcaattgc tttgataaaa catcatgagc aaaagcaact tggccaggaa ggaaggggtt
      241 tgttgccgtt tacagttaat aatccatcgt ggggagaagt cagggcagaa attcaaagca
      301 gaaacctgga gatagagggc tgaagcagaa gccatggagg aaagccgttt tggttccctg
      361 tggcttcctc agcctgcttc catatacaac ccgcctaggg ctaccagcct aatggtggcg
      421 ccacacacac tggtcgggtc cttccacatc actcattaat caagaaagtg cccagacttc
      481 cctacaggca acctgatgga agtctttact acaattaagg ttcctcttcc catttaactt
      541 cagttgaaga aaaaaaaaaa aaaaaaaaaa agcaaagcaa aaagcccaaa aacctaatca
      601 aaataccttc taaactacac ttcatgtaat agattagagt tgataaattg acgatgtatc
      661 tacagtatta tgaaatgctg aatatctgag atcggacctg tcttctccag agcttgggca
      721 aaggtgcccg ccccaaacag taaaaaaggc taacggcgtg atttctgctc ctttcagagc
      781 aggggactcc gattaacacc tgattgaagc tgggtgagag gcaggactgt gcctcggttg
      841 cctctcaccc agcccagcaa taactctcag ggcacacgga actggcttca gaccttccac
      901 acaacccata ggaagagccc tgggcgcgct cccgcgtcct agcctcttct cttccaccgc
      961 gagagcgcag cgcacgcgcg ccgtgcccgg cagagcgcgc gcccgcgagc tccccgcagc
     1021 cccgtgctca tccgggtccc cgcgagcctc ggccaatgga ggcggctccc ggccccggga
     1081 gcagaactat gcctcctccc ccttctcgcg gcctgtggcc cccgcgagcg gcctggggct
     1141 caggagaccc gcagaggctg cgagtccgga tcccacgcga gaactcaggt cgtgagcgcc
     1201 cagcgatcgc aaccggtccc gaagcaccgg gcgcggcggc gcacgcgggg gtctgagcca
     1261 gtgtagtaaa cacacttcag aaacgtgagg tgccggtggt cacgtggggt gcatgccgcc
     1321 caatgagaag ccgggggcgg ggcggaggcc gctgacgcgg cggagtcacc cgggcagcct
     1381 cgggaccggt gaccggcagg cgagcgtccc gcggcctgga gctctagccc gcgctctccg
     1441 agctcgctgc ccactccggg acgcacaggc cgcgtcgcgc ggaggccgcg tgagctcgtg
     1501 tccgttcgtg gaggccgcct cgggcgcgcc accgtcttct cgcgggtcgc ctcggttggg
     1561 cgaggtttcc gtgacgagcc tcccggggct gctgcgacca gcccgggccg tcgtggcggc
     1621 tcgggccccg cgcgctcgct tgcgctccgg gggtctcagg ccctggaagg tatgcgtgtc
     1681 ctccggacga gatctgcgat ctaagtgtcg acgatatcga attcataact tcgtatagca
     1741 tacattatac gaagttattc tttgcaccat tctaaagaat aacagtgata atttctgggt
     1801 taaggcaaat aacttcgtat aggatacttt atacgaagtt atgctagctt agacgttgat
     1861 cctggcgctg gcgcaagcag cagggtgtct atccatgccg ctctcctggg cgcagctcat
     1921 gggcagggtg ccggcggcct gctcctccac ctcgggaggg aagccgtggc tgttaaggtg
     1981 gatcacaaag tggcttaagc tactgaacca gttcttgcaa gcagaattca cggcgatctt
     2041 gccgcccttc ttggccttaa tgagaatctc gcggatcttg cgggcgtcca acttgccggt
     2101 cagtccttta ggcacctcgt ccacgaacac aacaccaccg cgcagcttct tggcggttgt
     2161 aacctggctg gccacatagt ccacgatctc cttctcggtc atggttttac cgtgttccag
     2221 cacgacgact gcggcgggca gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc
     2281 gtcgaagatg ttggggtgtt gcagcaggat gctctccagt tcggctgggg ctacctggta
     2341 gcccttgtat ttgatcaggc tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc
     2401 ccagtaggcg atgtcgccgc tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc
     2461 ctcggggttg ttaacgtagc cgctcatgat catggggcca cggacgcaca gctcgccgcg
     2521 ctggttcaca cccagtgtct taccggtgtc caagtccacc accttagcct cgaagaaggg
     2581 caccaccttg cctactgcgc caggcttgtc gtccccttcg ggggtgatca gaatggcgct
     2641 ggttgtttct gtcaggccgt agccctggcg gatgcctggt aggtggaagc gtttggccac
     2701 ggcctcacct acctccttgc tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct
     2761 taggtcgtac ttgtcgatga gagtgctctt agcgaagaag ctaaatagtg tgggcaccag
     2821 cagggcagat tgaatcttat agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg
     2881 gtacatgagc acgacccgaa agccgcagat caagtagccc agcgtggtga acatgccgaa
     2941 gccgtggtga aatggcacca cgctgaggat agcggtgtcg gggatgatct ggttgccgaa
     3001 gatggggtcg cgggcatgac tgaatcggac acaagcggtg cggtgcggta gggctacgcc
     3061 cttgggcaat ccggtactgc cactactgtt catgatcagg gcgatggttt tgtcccggtc
     3121 gaagctctcg ggcacgaagt cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa
     3181 ggtgtacatg ctttggaagc cctggtagtc ggtcttgcta tccatgatga tgatcttttg
     3241 tatgatcggt agcttctttt gcacgttgag gatcttttgc agccctttct tgctcacgaa
     3301 tacgacggtg ggctggctga tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc
     3361 gttagctggg gccacagcca caccgatgaa cagggcaccc aacacgggca tgaagaactg
     3421 caagctattc tcgctgcaca ccacgatccg atggtttgta ttcagcccat agcgcttcat
     3481 agcttctgcc agccgaacgc tcatctcgaa gtactcggcg taggtaatgt ccacctcgat
     3541 atgtgcgtcg gtaaaggcga tggtgccggg caccagggcg tagcgcttca tggctttgtg
     3601 cagctgctcg ccggcggtcc cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat
     3661 gtttttggca tcttcacctg cagggccctg aaaatacagg ttttcggatc ctgaaccgcc
     3721 tgccgatccc tcatgccact ctattttctg agcttcgaaa atatcgttca ggccgctagc
     3781 tgcggccgct ccacccttat cgtcatcgtc cttgtagtcg atgtcatgat ctttataatc
     3841 accgtcatgg tctttgtagt ccatggtggg tacctcttga cagctctacc ttttactact
     3901 ataaaaacgt acatgtagta acttccttat gccacttcca aaagatgatt tcaacatgaa
     3961 tcaacagaaa tagtttctaa agtaggtcat cgctatagca accagtgggt ggtttagcat
     4021 actcccatca caatgaccta ctttctagtc tgtgctagga cagcttatat aactagtatt
     4081 tctagttctg tctataaaat agtctgaaaa aggcaatggc aagatacaac ataatcccac
     4141 tgctactgaa aacaagacat tatgaattca tataacacag tcatctgtcc tatgccatag
     4201 tttctacact gtgtcataac aaccatctcg attagggata acagggtaat tcttgtcatc
     4261 gtcatccttg tagtccatgg tggcctctcc ttcgagtgca aaaagaacaa gtagcttgta
     4321 ttctatagtg tcacctaaat gctagcctat agtgagtcgt attaagtact ctagccttaa
     4381 gagctgtaat tgaactggga gtggacacct gtggagagaa aggcaaagtg gatgtcagta
     4441 agaccaatag gtgcctatca gaaacgcaag agtcttctct gtctcgacaa gcccagtttc
     4501 tattggtctc cttaaacctg tcttgtaacc ttgatactta cctgcccagt gcctcacgac
     4561 caactttcgc taccttagga ccgttatagt tactgcagcc ataacttcgt ataatgtatg
     4621 ctatacgaag ttattaagag gtttcatatt gctaatagca gctacaatcc agctaccatt
     4681 ctgcataact tcgtataaag tatcctatac gaagttattc cggaaagctt atcgataatc
     4741 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4801 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4861 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4921 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4981 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5041 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5101 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5161 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5221 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5281 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     5341 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct
     5401 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt
     5461 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg
     5521 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc
     5581 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc
     5641 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt
     5701 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca
     5761 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
     5821 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     5881 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     5941 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     6001 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     6061 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     6121 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     6181 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6241 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6301 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6361 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     6421 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     6481 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     6541 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6601 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6661 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6721 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8581 tggccttttg ctggcctttt gctcacatgt
//
