LOCUS       sequence-218579-        6183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6183)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos-M-ALK5-N-13
FEATURES             Location/Qualifiers
     source          1..6183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2223..2903
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3025..3146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3062..3081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3116..3135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3153..3608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3290..3311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3502..3521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3635..3739
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3741..4098
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3741..3932
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3764..3784)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3949..4084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4133..4927
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4133..4927
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4187..4206)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4797..4816
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5115..5134)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5159..5206
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5535..6123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6024..6043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctgc caccatggag gcggcggtcg ctgctccgcg tccccggctg ctcctcctcg
      721 tgctggcggc ggcggcggcg gcggcggcgg cgctgctccc gggggcgacg gcgttacagt
      781 gtttctgcca cctctgtaca aaagacaatt ttacttgtgt gacagatggg ctctgctttg
      841 tctctgtcac agagaccaca gacaaagtta tacacaacag catgtgtata gctgaaattg
      901 acttaattcc tcgagatagg ccgtttgtat gtgcaccctc ttcaaaaact gggtctgtga
      961 ctacaacata ttgctgcaat caggaccatt gcaataaaat agaacttcca actactgtaa
     1021 agtcatcacc tggccttggt cctgtggaac tggcagctgt cattgctgga ccagtgtgct
     1081 tcgtctgcat ctcactcatg ttgatggtct atatctgcca caaccgcact gtcattcacc
     1141 atcgagtgcc aaatgaagag gacccttcat tagatcgccc ttttatttca gagggtacta
     1201 cgttgaaaga cttaatttat gatatgacaa cgtcaggttc tggctcaggt ttaccattgc
     1261 ttgttcagag aacaattgcg agaactattg tgttacaaga aagcattggc aaaggtcgat
     1321 ttggagaagt ttggagagga aagtggcggg gagaagaagt tgctgttaag atattctcct
     1381 ctagagaaga acgttcgtgg ttccgtgagg cagagattta tcaaactgta atgttacgtc
     1441 atgaaaacat cctgggattt atagcagcag acaataaaga caatggtact tggactcagc
     1501 tctggttggt gtcagattat catgagcatg gatccctttt tgattactta aacagataca
     1561 cagttactgt ggaaggaatg ataaaacttg ctctgtccac ggcgagcggt cttgcccatc
     1621 ttcacatgga gattgttggt acccaaggaa agccagccat tgctcataga gatttgaaat
     1681 caaagaatat cttggtaaag aagaatggaa cttgctgtat tgcagactta ggactggcag
     1741 taagacatga ttcagccaca gataccattg atattgctcc aaaccacaga gtgggaacaa
     1801 aaaggtacat ggcccctgaa gttctcgatg attccataaa tatgaaacat tttgaatcct
     1861 tcaaacgtgc tgacatctat gcaatgggct tagtattctg ggaaattgct cgacgatgtt
     1921 ccattggtgg aattcatgaa gattaccaac tgccttatta tgatcttgta ccttctgacc
     1981 catcagttga agaaatgaga aaagttgttt gtgaacagaa gttaaggcca aatatcccaa
     2041 acagatggca gagctgtgaa gccttgagag taatggctaa aattatgaga gaatgttggt
     2101 atgccaatgg agcagctagg cttacagcat tgcggattaa gaaaacatta tcgcaactca
     2161 gtcaacagga aggcatcaaa atgggttccg ggggtgcggg cggcggagga ccggtcgcca
     2221 ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc aacgtaaacg
     2281 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta
     2341 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
     2401 cattcatgta cggcaacagg gtattcgcca aatatccaga caacatacaa gactatttta
     2461 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
     2521 tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
     2581 tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
     2641 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat attcatatgg
     2701 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
     2761 aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt gagattttaa
     2821 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
     2881 tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag ccataccaca
     2941 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3001 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3061 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3121 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3181 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3241 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3301 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3361 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3421 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3481 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3541 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3601 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3661 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3721 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3781 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3841 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3901 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3961 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4021 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4081 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4141 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4201 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4261 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4321 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4381 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4441 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4501 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4561 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4621 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4681 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4741 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4801 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4861 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4921 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4981 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5041 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5101 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5161 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5221 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5281 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5341 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6181 atg
//
