LOCUS       sequence-218580-        8586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human HOIP with C-terminal strep-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Hulsmann J, Kravic B, Weith M, Gstaiger M, Aebersold RH, Collins BC,
            Meyer H
  TITLE     AP-SWATH reveals direct involvement of VCP/p97 in integrated stress 
            response signaling through facilitating CReP/PPP1R15B degradation.
  JOURNAL   Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 
            10.1074/mcp.RA117.000471.
  PUBMED    29599191
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 
            10.1074/mcp.RA117.000471."
COMMENT     Sequence Label:  pcDNA5FRT/TO-HOIP-Strep-HA
FEATURES             Location/Qualifiers
     source          1..8586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        480..683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     634..654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    685..703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    706..724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    901..925
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4148..4172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4219..4242
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4279..4302
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4333..4359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4333..4359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4403..4420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4409..4633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4766..4785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4917..4964
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4972..5994
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4972..5994
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6124..6245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6161..6180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6215..6234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6294..6310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6294..6310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6307..6329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6318..6334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6342..6348,6349..6366,6367..6372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6387..6408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6525..6542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6696..7284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6776..6795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7455..8246,8247..8315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8078..8097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8316..8420)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8495..8514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
      721 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      781 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac
      841 ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca
      901 acaagtttgt acaaaaaagc aggctccacc atgccggggg aggaagagga gcgggccttc
      961 ctggtggccc gcgaggagct ggcgagcgcc ctgaggaggg attccgggca ggcgttttcc
     1021 ctggagcagc tccggccgct actagccagc tctctgccgc tagccgcccg ctacctgcag
     1081 ctggacgccg cacgccttgt ccgctgcaac gctcatgggg agccccgaaa ctacctcaac
     1141 accctgtcca cggctctgaa catcctggag aaatacggcc gcaaccttct cagccctcag
     1201 cggcctcggt actggcgtgg tgtcaagttt aataaccctg tctttcgcag cacggtggat
     1261 gctgtgcagg ggggccgaga tgtgctgcga ttatatggct acacagagga gcaaccagat
     1321 gggttgagct tccccgaagg gcaggaggag ccagatgagc accaggttgc tacagtcaca
     1381 ctggaagtac tgctgcttcg gacagagctc agcctgctat tgcagaatac tcatccaaga
     1441 cagcaggcac tggagcagct gttggaagac aaggttgaag atgatatgct gcagctttca
     1501 gaatttgacc ccctattgag agagattgct cctggccccc tcaccacacc ctctgtccca
     1561 ggctccactc ctggtccctg cttcctctgt ggttctgccc caggcacact gcactgccca
     1621 tcctgtaaac aggccctgtg tccagcctgt gaccacctgt tccatggaca cccatcccgt
     1681 gctcatcacc tccgccagac cctgcctggg gtcctgcagg gtacccacct gagccccagt
     1741 ttacctgcct cagcccaacc acggccccag tcgacctccc tgctggccct gggagacagc
     1801 tctctttctt cccctaatcc tgcaagtgct catttgccct ggcactgtgc tgcctgtgcc
     1861 atgctaaatg agccttgggc agtgctctgt gtggcctgtg atcggccccg aggctgtaag
     1921 gggttggggt tgggaactga gggtccccaa ggaactggag gcctagaacc tgatcttgca
     1981 cggggtcggt gggcctgcca gagctgtacc tttgagaatg aggcagctgc tgtgctatgt
     2041 tccatatgtg agcgacctcg gctggcccag cctcccagct tggtggtgga ttcccgagat
     2101 gctggcattt gcctgcaacc ccttcagcag ggggatgctt tgctggcctc tgcccagagt
     2161 caagtctggt actgtattca ctgtaccttc tgcaactcga gccctggctg ggtgtgtgtt
     2221 atgtgcaacc ggactagtag ccccattcca gcacaacatg ccccccggcc ctatgccagc
     2281 tctttggaaa agggaccccc caagcctggg cccccacgac gccttagtgc ccccctgccc
     2341 agttcctgtg gagatcctga gaagcagcgc caagacaaga tgcgggaaga aggcctccag
     2401 ctagtgagca tgatccggga aggggaagcc gcaggtgcct gtccagagga gatcttctcg
     2461 gctctgcagt actcgggcac tgaggtgcct ctgcagtggt tgcgctcaga actgccctac
     2521 gtcctggaga tggtggctga gctggctgga cagcaggacc ctgggctggg tgccttttcc
     2581 tgtcaggagg cccggagagc ctggctggat cgtcatggca accttgatga agctgtggag
     2641 gagtgtgtga ggaccaggcg aaggaaggtg caggagctcc agtctctagg ctttgggcct
     2701 gaggaggggt ctctccaggc attgttccag cacggaggtg atgtgtcacg ggccctgact
     2761 gagctacagc gccaacgcct agagcccttc cgccagcgcc tctgggacag tggccctgag
     2821 cccacccctt cctgggatgg gccagacaag cagagcctgg tcaggcggct tttggcagtc
     2881 tacgcactcc ccagctgggg ccgggcagag ctggcactgt cactgctgca ggagacaccc
     2941 aggaactatg agttggggga tgtggtagaa gctgtgaggc acagccagga ccgggccttc
     3001 ctgcgccgct tgcttgccca ggagtgtgcc gtgtgtggct gggccctgcc ccacaaccgg
     3061 atgcaggccc tgacttcctg tgagtgcacc atctgtcctg actgcttccg ccagcacttc
     3121 accatcgcct tgaaggagaa gcacatcaca gacatggtgt gccctgcctg tggccgcccc
     3181 gacctcaccg atgacacaca gttgctcagc tacttctcta cccttgacat ccagcttcgc
     3241 gagagcctag agccagatgc ctatgcgttg ttccataaga agctgaccga gggtgtgctg
     3301 atgcgggacc ccaagttctt gtggtgtgcc cagtgctcct ttggcttcat atatgagcgt
     3361 gagcagctgg aggcaacttg tccccagtgt caccagacct tctgtgtgcg ctgcaagcgc
     3421 cagtgggagg agcagcaccg aggtcggagc tgtgaggact tccagaactg gaaacgcatg
     3481 aacgacccag aataccaggc ccagggccta gcaatgtatc ttcaggaaaa cggcattgac
     3541 tgccccaaat gcaagttctc gtacgccctg gcccgaggag gctgcatgca ctttcactgt
     3601 acccagtgcc gccaccagtt ctgcagcggc tgctacaatg ccttttacgc caagaataaa
     3661 tgtccagagc ctaactgcag ggtgaaaaag tccctgcacg gccaccaccc tcgagactgc
     3721 ctcttctacc tgcgggactg gactgctctc cggcttcaga agctgctaca ggacaataac
     3781 gtcatgttta atacagagcc tccagctggg gcccgggcag tccctggagg cggctgccga
     3841 gtgatagagc agaaggaggt tcccaatggg ctcagggacg aagcttgtgg caaggaaact
     3901 ccagctggct atgccggcct gtgccaggca cactacaaag agtatcttgt gagcctcatc
     3961 aatgcccact cgctggaccc agccaccttg tatgaggtgg aagagctgga gacggccact
     4021 gagcgctacc tgcacgtacg cccccagcct ttggctggag aggatccccc tgcttaccag
     4081 gcccgcttgt tacagaagct gacagaagag gtacccttgg gacagagtat cccccgcagg
     4141 cggaaggacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     4201 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     4261 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     4321 accatggcca gctaccccta cgacgtgccc gactacgcct gatgactcga gtctagaggg
     4381 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     4921 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     4981 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5041 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5101 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5161 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5221 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5281 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5341 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5401 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5461 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5521 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5581 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5641 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5701 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5761 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5821 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5881 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5941 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     6001 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6061 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6121 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6241 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6481 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8461 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8521 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8581 tttaag
//
