LOCUS       sequence-218583-        5739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5739)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..5739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             671..1348
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2657..2778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2694..2713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2748..2767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2785..3240)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2922..2943)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3134..3153
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3267..3371
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3396..3416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3440..3650
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3462..3658
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3509..3644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3693..4487
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3693..4487
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3747..3766)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4357..4376
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4675..4694)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4719..4766
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5095..5683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5584..5603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggtcgccacc atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa
      721 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa
      781 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct
      841 gaccactgca ttcctgtacg gcaacagggt attcgccaaa tatccagaca acatacaaga
      901 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga
      961 cgggggcatt tgcaacgcca gaaacgacat aacaatggaa ggggacactt tctataataa
     1021 agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct
     1081 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatgt
     1141 agagatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta
     1201 caaagctaag gagaagggtg tcaagttacc aggcgcccac tttgtggacc actgcattga
     1261 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca
     1321 ttctggattg cctgacaatg ccagacgatc cggactcaga tctggcagcg gtggaggcag
     1381 cgcatccggc ggaagcggaa gcgatgatga tatcgccgcg ctcgtcgtcg acaacggctc
     1441 cggcatgtgc aaggccggct tcgcgggcga cgatgccccc cgggccgtct tcccctccat
     1501 cgtggggcgc cccaggcacc agggcgtgat ggtgggcatg ggtcagaagg attcctatgt
     1561 gggcgacgag gcccagagca agagaggcat cctcaccctg aagtacccca tcgagcacgg
     1621 catcgtcacc aactgggacg acatggagaa aatctggcac cacaccttct acaatgagct
     1681 gcgtgtggct cccgaggagc accccgtgct gctgaccgag gcccccctga accccaaggc
     1741 caaccgcgag aagatgaccc agatcatgtt tgagaccttc aacaccccag ccatgtacgt
     1801 tgctatccag gctgtgctat ccctgtacgc ctctggccgt accactggca tcgtgatgga
     1861 ctccggtgac ggggtcaccc acactgtgcc catctacgag gggtatgccc tcccccatgc
     1921 catcctgcgt ctggacctgg ctggccggga cctgactgac tacctcatga agatcctcac
     1981 cgagcgcggc tacagcttca ccaccacggc cgagcgggaa atcgtgcgtg acattaagga
     2041 gaagctgtgc tacgtcgccc tggacttcga gcaagagatg gccacggctg cttccagctc
     2101 ctccctggag aagagctacg agctgcctga cggccaggtc atcaccattg gcaatgagcg
     2161 gttccgctgc cctgaggcac tcttccagcc ttccttcctg ggcatggagt cctgtggcat
     2221 ccacgaaact accttcaact ccatcatgaa gtgtgacgtg gacatccgca aagacctgta
     2281 cgccaacaca gtgctgtctg gcggcaccac catgtaccct ggcattgccg acaggatgca
     2341 gaaggagatc actgccctgg cacccagcac aatgaagatc aagatcattg ctcctcctga
     2401 gcgcaagtac tccgtgtgga tcggcggctc catcctggcc tcgctgtcca ccttccagca
     2461 gatgtggatc agcaagcagg agtatgacga gtccggcccc tccatcgtcc accgcaaatg
     2521 cttctaggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     2581 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2641 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2761 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     2821 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2881 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2941 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3001 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3061 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3121 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3181 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3241 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3301 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3361 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3481 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3541 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3661 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3721 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3781 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3841 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     3901 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3961 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4021 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4081 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4141 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4201 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4261 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4321 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4381 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4441 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4501 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4561 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4621 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4681 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4741 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4801 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4861 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4921 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     4981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5701 ctttttacgg ttcctggcct tttgctggcc ttttgctca
//
