LOCUS       sequence-218615-        6457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 503 / 569, 
            Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6457)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..6457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2501..3181
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3303..3424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3340..3359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3394..3413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3431..3886)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3568..3589)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3780..3799
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3913..4017
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4019..4376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4019..4210
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4042..4062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4227..4362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4411..5205
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4411..5205
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4465..4484)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5075..5094
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5393..5412)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5437..5484
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5813..6401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6302..6321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagaccg ccatggaagg gaagtggttg ctgtgtatgt tactggtgct
      721 tggaactgct attgttgagg ctcatgatgg acatgatgat gatgtgattg atattgagga
      781 tgaccttgac gatgtcattg aagaggtaga agactcaaaa ccagatacca ctgctcctcc
      841 ttcatctccc aaggttactt acaaagctcc agttccaaca ggggaagtat attttgctga
      901 ttcttttgac agaggaactc tgtcagggtg gattttatcc aaagccaaga aagacgatac
      961 cgatgatgaa attgccaaat atgatggaaa gtgggaggta gaggaaatga aggagtcaaa
     1021 gcttccaggt gataaaggac ttgtgttgat gtctcgggcc aagcatcatg ccatctctgc
     1081 taaactgaac aagcccttcc tgtttgacac caagcctctc attgttcagt atgaggttaa
     1141 tttccaaaat ggaatagaat gtggtggtgc ctatgtgaaa ctgctttcta aaacaccaga
     1201 actcaacctg gatcagttcc atgacaagac cccttatacg attatgtttg gtccagataa
     1261 atgtggagag gactataaac tgcacttcat cttccgacac aaaaacccca aaacgggtat
     1321 ctatgaagaa aaacatgcta agaggccaga tgcagatctg aagacctatt ttactgataa
     1381 gaaaacacat ctttacacac taatcttgaa tccagataat agttttgaaa tactggttga
     1441 ccaatctgtg gtgaatagtg gaaatctgct caatgacatg actcctcctg taaatccttc
     1501 acgtgaaatt gaggacccag aagaccggaa gcccgaggat tgggatgaaa gaccaaaaat
     1561 cccagatcca gaagctgtca agccagatga ctgggatgaa gatgcccctg ctaagattcc
     1621 agatgaagag gccacaaaac ccgaaggctg gttagatgat gagcctgagt acgtacctga
     1681 tccagacgca gagaaacctg aggattggga tgaagacatg gatggagaat gggaggctcc
     1741 tcagattgcc aaccctagat gtgagtcagc tcctggatgt ggtgtctggc agcgacctgt
     1801 gattgacaac cccaattata aaggcaaatg gaagcctcct atgattgaca atcccagtta
     1861 ccagggaatc tggaaaccca ggaaaatacc aaatccagat ttctttgaag atctggaacc
     1921 tttcagaatg actcctttta gtgctattgg tttggagctg tggtccatga cctctgacat
     1981 tttttttgac aactttatca tttgtgctga tcgaagaata gttgatgatt gggccaatga
     2041 tggatggggc ctgaagaaag ctgctgatgg ggctgctgag ccaggcgttg tggggcagat
     2101 gaacgaggca gctgaagagc gcccgtggct gtgggtagtc tatattctaa ctgtagccct
     2161 tcctgtgttc ctggttatcc tcttctgctg ttctggaaag aaacagacca gtggtatgga
     2221 gtataagaaa actgatgcac ctcaaccgga tgtgaaggaa gaggaagaag agaaggaaga
     2281 ggaaaaggac aagggagatg aggaggagga aggagaagag aaacttgaag agaaacagaa
     2341 aagtgatgct gaagaagatg gtggcactgt cagtcaagag gaggaagaca gaaaacctaa
     2401 agcagaggag gatgaaattt tgaacagatc accaagaaac agaaagccac gaagagaggg
     2461 gggtgcgtca ggcggcagcg gatctggacc ggtcgccacc atgagtgcga ttaagccaga
     2521 catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg
     2581 agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg
     2641 cggacctctg ccttttgcct ttgatatcct gaccactgca ttcctgtacg gcaacagggt
     2701 attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta
     2761 ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat
     2821 aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca actttcccgc
     2881 caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta
     2941 tgtgcgtgat ggagtgctga cgggtgatgt agagatggct ttgttgcttg aaggaaatgc
     3001 ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc
     3061 aggcgcccac tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa
     3121 ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata
     3181 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3241 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3301 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3421 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3481 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3541 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3601 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3661 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3721 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3781 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3841 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     4021 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4381 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4441 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4501 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4561 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4621 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4681 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4741 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4801 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4861 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4921 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4981 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5041 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5101 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5161 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5221 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5281 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5341 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     5401 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5461 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5521 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5581 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5641 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6421 ttttacggtt cctggccttt tgctggcctt ttgctca
//
