LOCUS       sequence-218619-        5903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5903)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..5903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        163..366
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     317..337
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             411..1088
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2754..2875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2791..2810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2845..2864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2882..3337)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3019..3040)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3231..3250
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3364..3468
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3493..3513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3537..3747
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3559..3755
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3606..3741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3790..4584
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3790..4584
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3844..3863)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4454..4473
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4772..4791)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4816..4863
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5192..5780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5681..5700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      121 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      181 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      241 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      301 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      361 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atgagtgcga
      421 ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg
      481 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
      541 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttcctgtacg
      601 gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
      661 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca
      721 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
      781 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
      841 agaaaatgta tgtgcgtgat ggagtgctga cgggtgatgt agagatggct ttgttgcttg
      901 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
      961 tcaagttacc aggcgcccac tttgtggacc actgcattga gattttaagc catgacaaag
     1021 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     1081 ccagacgatc cggaagtgct gggagcgcaa gcggaagcgg aggctccggt tttaactacg
     1141 aacgtccaaa acacttcatc cagtcccaaa acccatgtgg ctccagattg cagcctcctg
     1201 gaccagaaac ctccagcttc tctagccaga ccaaacagtc ttccattatc atccagcccc
     1261 gccagtgtac agagcaaaga ttttctgcct cctcaacact gagctctcac atcaccatgt
     1321 cctcctctgc tttccctgct tctccccagc agcatgctgg ctccaaccca ggccaaaggg
     1381 ttacaaccac ctataaccag tccccagcca gcttcctcag ctccatatta ccatcacagc
     1441 ctgattacaa tagcagtaaa atcccttccg ctatggattc caactatcaa cagtcctcag
     1501 ctggccaacc tataaatgca aagccatccc aaactgcaaa tgctaagccc ataccaagaa
     1561 ctcctgatca tgaaatacaa ggatcaaaag aagctttgat tcaagatttg gaaagaaagc
     1621 tgaaatgcaa ggacaccctt cttcataatg gaaatcaacg tctaacatat gaagagaaga
     1681 tggctcgcag attgctagga ccacagaatg cagctgctgt gtttcaagct caggatgaca
     1741 gtggtgcaca agactcgcag caacacaact cagaacatgc gcgactgcaa gttcctacat
     1801 cacaagtaag aagtagatca acctcaaggg gagatgtgaa tgatcaggat gcaatccagg
     1861 agaaatttta cccaccacgt ttcattcaag tgccagagaa catgtcgatt gatgaaggaa
     1921 gattctgcag aatggacttc aaagtgagtg gactgccagc tcctgatgtg tcatggtatc
     1981 taaatggaag aacagttcaa tcagatgatt tgcacaaaat gatagtgtct gagaagggtc
     2041 ttcattcact catctttgaa gtagtcagag cttcagatgc aggggcttat gcatgtgttg
     2101 ccaagaatag agcaggagaa gccaccttca ctgtgcagct ggatgtcctt gcaaaagaac
     2161 ataaaagagc accaatgttt atctacaaac cacagagcaa aaaagtttta gagggagatt
     2221 cagtgaaact agaatgccag atctcggcta tacctccacc aaagcttttc tggaaaagaa
     2281 ataatgaaat ggtacaattc aacactgacc gaataagctt atatcaagat aacactggaa
     2341 gagttacttt actgataaaa gatgtaaaca agaaagatgc tgggtggtat actgtgtcag
     2401 cagttaatga agctggagtg actacatgta acacaagatt agacgttacg gcacgtccaa
     2461 accaaactct tccagctcct aagcagttac gggttcgacc aacattcagc aaatatttag
     2521 cacttaatgg gaaaggtttg aatgtaaaac aagcttttaa cccagaagga gaatttcagc
     2581 gtttggcagc tcaatctgga ctctatgaaa gtgaagaact ttaaggatcc accggatcta
     2641 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2701 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     2881 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2941 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3001 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3061 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3121 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3181 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3241 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3301 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3361 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3421 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3481 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3601 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3661 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3721 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     3781 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     3841 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     3901 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     3961 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4021 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4081 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4141 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4201 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4261 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     4321 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4381 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4441 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4501 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4561 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     4621 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     4681 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     4741 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     4801 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     4861 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     4921 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     4981 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     5041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5881 ttaccgccat gcattagtta tta
//
