LOCUS       sequence-21862-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: His-FLAG-Med18 pBacPAK8
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aataaaaaaa cctataaata ccatggggtc atcatcatca ccatcacggt ggtcgacaag
       61 cttggatccc tgcaggcctc gagttcgaat ctagcatgcc agactacaag gacgatgatg
      121 acaagctcga gatggaggca cctccagtca ccatgatgcc tgtcactggg ggcaccatta
      181 acatgatgga gtacctgttg cagggaagtg ttttagatca cagtttggaa agcctcatcc
      241 accgccttcg tggtttgtgt gacaacatgg aacctgagac tttccttgac catgagatgg
      301 tattcctcct taagggccag caagccagcc catttgttct cagggcccga cgctctatgg
      361 acagggcagg ggcaccctgg catctgcgct acctgggaca gccagaaatg ggagacaaga
      421 accgccatgc cctggtgcga aactgcgtgg acattgccac atctgagaac ctcaccgact
      481 tcttgatgga aatgggcttc cgcatggacc atgagtttgt tgctaaggga catttgttcc
      541 gtaagggcat catgaagatt atggtgtaca agattttccg catcctggtg ccagggaaca
      601 cagacagcac tgaggccttg tcactctcct atctcgtgga attaagtgtg gtagcacccg
      661 ctgggcagga catggtctct gatgacatga agaacttcgc agaacagcta aaacctctgg
      721 ttcacct
//
