LOCUS       sequence-218628-        4428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encoding the RFP gene under blue light-inducible promoter 
            BindF1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL
  TITLE     Cell-Free Optogenetic Gene Expression System.
  JOURNAL   ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 
            10.1021/acssynbio.7b00422. Epub 2018 Apr 4.
  PUBMED    29596741
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00422"; journalName: "ACS Synth Biol"; date: 
            "2018-04-20- 20"; volume: "7"; issue: "4"; pages: "986-994"
COMMENT     Sequence Label: pBLind
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             124..801
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      834..905
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      921..948
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1106..1694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1186..1205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2023..2070
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2095..2114
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2302..3096)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2302..3096)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2413..2432)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3023..3042
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3239..4117)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4144..4428
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4351..4370
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 acccgttttt ttgggaattc aaaagatctt ttaagttgac aggtagcctt tagtccatgt
       61 tagcgaagaa aatggtttgt tatagtcgaa taaatatgtc tagagaaaga ggagaaatac
      121 tagatggcga gtagcgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      181 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      241 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      301 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      361 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      421 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      481 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      541 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      601 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      661 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      721 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      781 cgtcactcca ccggtgctta aggatccaaa ctcgagtaag gatctccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctagg
      961 gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta
     1861 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
     1921 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
     1981 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
     2041 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     2101 ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc
     2161 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     2221 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     2281 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     2341 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     2401 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     2461 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     2521 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     2581 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     2641 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     2701 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     2761 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     2821 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     2881 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     2941 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     3001 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     3061 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     3121 ttgatcagat catgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     3181 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtctt
     3241 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     3301 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     3361 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     3421 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     3481 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     3541 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     3601 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     3661 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     3721 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     3781 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     3841 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     3901 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     3961 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     4021 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     4081 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     4141 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc
     4201 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
     4261 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca
     4321 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag
     4381 cggattctac ctgacgcttt ttatcgcaac tctctactgt ttctccat
//
