LOCUS       sequence-218629-        5986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of GST-tagged p37 lacking the UBX domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I,
            Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, 
            Meyer H
  TITLE     Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives
            PP1 Holoenzyme Formation.
  JOURNAL   Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 
            10.1016/j.molcel.2018.09.020.
  PUBMED    30344098
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 
            10.1016/j.molcel.2018.09.020."
COMMENT     Sequence Label: pGEX-6P-1 p37 deltaUBX
FEATURES             Location/Qualifiers
     source          1..5986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(100..105,106..121,122..128)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             175..828
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     786..808
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             835..858
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1953..1975)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2120..2138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2206..2310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2311..2379,2380..3171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2529..2548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3342..3930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3831..3850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4084..4101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4174..4251
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4252..5334
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4271..4290)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5383..5388,5389..5406,5407..5413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5421..5437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5445..5461
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5445..5461
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5457..5984
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(5477..5494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5477..5493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5486..5508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
       61 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc
      121 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc
      181 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      241 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      301 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      361 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
      421 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
      481 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
      541 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
      601 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
      661 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
      721 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
      781 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggaa
      841 gttctgttcc aggggcccct gggatccatg gcggagggtg gccgtgcgga gccggaggag
      901 caggagcgtg ggtcctccag gccacggccc ccaagtgcgc gggacttaca gttggccttg
      961 gcagaattat atgaagatga aatgaaatgc aagtcttcca agcctgacag atccacacct
     1021 gcaacctgta ggagcccacg aacaccgcca catcggcttt attcaggtga tcacaaatat
     1081 gatggattac atatagttca acctccaact gggaaaattg tgaatgaact tttcaaagag
     1141 gcaagggaac atggagctgt ccctctaaac gaagccacaa gatcttcaag ggaagacaaa
     1201 actaagtcat ttacaggtgg aggatacaga ttggggaatt ccttctacaa gcgatcagag
     1261 tatatctatg gggaaaatca gctccaagat gttcaggttt tactgaagct gtggcgcaat
     1321 ggtttcagtt tagacgatgg agaattgagg ccttacagtg acccaacaaa tgctcagttt
     1381 ttggaatctg ttaagagagg agagactccc ctggagcttc agcgactggt ccatggcgcc
     1441 caagtgaacc tggatatgga ggatcatcaa gaccaagagt acataaaacc caggttgcga
     1501 ttcaaggctt ttagtggaga agggcaaaaa cttggaagcc ttacacctga aatcgtcagt
     1561 actccttcct ccccagaaga agaggataaa tcaatactga atgcagctgt tcttatttag
     1621 gattcaatgc ccaccacaaa aattcagatc aggttggcag atggaagtcg tctcgtacag
     1681 agattcaata gcacacaccg gattctggat gtacgagact ttatagtacg gtcacgtcct
     1741 gaatttgcga ctactgactt tattcttgtg acttcatttc caagtaaaga actaacagat
     1801 gaaaccgtga cactacaaga agcggacatt cttaacactg tgatactcca acagctaaaa
     1861 tagggatccc cggaattccc gggtcgactc gagcggccgc atcgtgactg actgacgatc
     1921 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1981 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     2041 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     2101 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2161 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2281 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2341 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2401 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2461 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2521 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     2581 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2641 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2701 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2761 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2821 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     2881 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2941 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3001 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3061 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3121 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3481 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4081 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     4141 catctgtgcg gtatttcaca ccgcataaat tccgacacca tcgaatggcg caaaaccttt
     4201 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     4261 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     4321 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     4381 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     4441 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     4501 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     4561 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     4621 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     4681 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     4741 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     4801 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     4861 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     4921 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     4981 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     5041 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     5101 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     5161 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     5221 gtgaaaagaa aaaccacgct agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5281 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5341 cgcaattaat gtaagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     5401 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     5461 ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5521 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5581 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg
     5641 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg
     5701 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca
     5761 ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg
     5821 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
     5881 ttatttttga tggcgttgga attagcttat cgactgcacg gtgcaccaat gcttctggcg
     5941 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaat
//
