LOCUS       sequence-21863-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: His-FLAG-Med20 pBacPAK8
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acagtttttg tataaaaaac ctataaatac catgggtcat catcatcacc atcacggtgg
       61 tcgacaagct tggatccctg caggcctcga gttcgaatct agcatgccag actacaagga
      121 cgatgatgac aagctcgaga tgggagtgac gtgtgtgtcc cagatgcctg tggctgaggg
      181 caagagtctc cagcagacag tggagctcct caccaagaaa ctggagatgc ttggagcaga
      241 gaaacaaggg acattttgtg tggactgtga gacttaccac acagctgcct ctaccctggg
      301 cagccaaggc caggctggga agctgatgta cgtgatgcat aactctgagt acccactgag
      361 ctgctttgcc ctctttgaga atggcccttg cctcattgct gacaccaact tcgatgtgct
      421 tatggtgaag ctcaagggct tcttccagag tgccaaggcc agcaagattg agacccgggg
      481 cactcgctac cagtattgtg acttcctggt gaaggtgggc acggtcacaa tggggcccag
      541 tgcccgtggc atctctgtgg aggtggagta tggcccttgt gtggtggcta gtgactgctg
      601 gagcctcctg cttgagttcc tacagagttt tctagggagc cacgcaccag gggcacccac
      661 agtatttggg aacagacacg atgcggttta tgggccagca gataccatga tacagtacat
      721 ggaactct
//
