LOCUS       sequence-218633-       10944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Galactose-inducible Ty3 with extra PPT- used for random bar code 
            library.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKN3097
FEATURES             Location/Qualifiers
     source          1..10944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    190..307
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    6411..7573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      6736..7573
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(7908..8711)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8712..8927)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8952..8970)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9038..9142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9143..9211,9212..10003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9361..9380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10174..10762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10663..10682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10916..10933
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacccgc ggatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
       61 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
      121 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
      181 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
      241 agacgaggac gcacggagga gagtctttct tcggagggct gtcacccgct cggcggcttc
      301 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
      361 aggttttttt aggctaatcg acctcgactc tagaggatcc ccctgaacta cccaaagtat
      421 aaatgcctga acaattagtt tagatccgag attccgcgct tccaccactt agtatgattc
      481 atattttata taatatataa gataagtaac attccgtgaa ttaatctgat aaactgtttt
      541 gacaactggt tacttcccta agactgttta tattaggatt gtcaagacac tccggtatta
      601 ctcgagcccg taatacaaca cctggtagcg ttaaaggtta ctaattgttc aaacgaacca
      661 tcgaaaagcc gaacctagct acaccacacc ccagtatgag ctttatggat caaatcccag
      721 gaggaggaaa ttatccaaaa ctcccagtag aatgccttcc taacttcccg atccaaccat
      781 ctttgacctt cagaggtaga aatgactcgc ataaactgaa aaactttatc tccgaaataa
      841 tgttaaacat gtctatgata tcttggccga atgatgccag tcgtattgtg tactgcagaa
      901 gacatttatt aaaccccgct gctcagtggg ctaatgactt tgtacaagaa caaggtatac
      961 ttgaaataac attcgacaca ttcatacaag gattatatca gcatttctat aagccaccag
     1021 atatcaataa aatctttaat gcaatcacgc aactttccga agctaaactt ggtattgagc
     1081 gtctcaacca acgattcaga aagatttggg acagaatgcc accagacttc atgaccgaaa
     1141 aagctgccat aatgacatat actaggctat tgacaaagga aacctataat attgtcagaa
     1201 tgcacaaacc agagacatta aaagacgcca tggaagaggc ttaccagaca actgcactaa
     1261 ctgaaagatt cttcccagga ttcgaacttg atgctgatgg agacactatc atcggtgcca
     1321 caacccactt acaagaagaa tacgactctg actatgattc agaagataat ctgacccaga
     1381 atggatacgt ccataccgta aggacaagaa gatcttacaa taaaccaatg tcaaatcatc
     1441 gaaacaggag aaataacaac ccatctagag aagaatgtat aaaaaatcgg ctatgcttct
     1501 attgtaagaa agagggacat cgcctgaacg aatgtagagc acgtaaggcg agttctaacc
     1561 gatcttgaac tcgaatcaaa agaccaacaa actcctttta tcaaaacctt accaattgta
     1621 cactatatcg ccatccccga gatggacaat accgccgaaa aaaccataaa aatacaaaac
     1681 acgaaagtaa aaaccctgtt tgacagtgga tcacccacgt catttatccg aagagatatt
     1741 gtagaacttc tcaaatacga aatctacgag acccctccac tccgttttag aggattcgta
     1801 gccaccaaat ccgccgttac atccgaagca gtcaccattg acctcaaaat caatgacctg
     1861 catataactt tagccgcgta catactggat aacatggact accaattgtt aattggaaat
     1921 ccaatcttac gccgctaccc gaaaatcctg cacacagtac tgaataccag agagagcccc
     1981 gactccttaa agcccaagac ttatcgctcc gaaaccgtta ataacgttag aacctactcc
     2041 gctggtaatc gtggtaaccc cagaaacata aaactgtctt ttgcccccac cattctcgaa
     2101 gcaactgacc cgaaatccgc tggtaatcgt ggtgactcca gaaccaaaac cctgtctctt
     2161 gcaaccacta ctcctgcagc aattgacccg cttacgaccc ttgataaccc aggtagtact
     2221 caaagtacat ttgcgcaatt cccgatacct gaagaagcga gcatcctaga agaggatgga
     2281 aaatactcca acgttgtctc aaccattcag agtgtagaac ctaatgctac tgatcacagc
     2341 aataaggaca ccttttgcac tttgccagtt tggttacaac agaagtatag agagatcata
     2401 cgtaatgatc tcccaccaag acctgccgac attaataaca tccccgtaaa acatgatatt
     2461 gaaattaaac ctggcgcaag actacctcga ctacagccat accatgttac agaaaagaac
     2521 gaacaagaaa tcaacaaaat agttcaaaaa ctgctcgata acaagttcat tgttccctca
     2581 aagtcgcctt gcagctcccc tgtagtcctc gtcccgaaga aagacggtac cttccgactc
     2641 tgcgtcgatt accgcaccct gaacaaagct accatctccg acccattccc attacccaga
     2701 atcgacaacc tattgagccg tattggaaat gcccagatat ttaccacgct agatttgcat
     2761 agtggttacc accagatccc gatggaaccc aaagaccgct acaaaaccgc ctttgtcaca
     2821 ccatccggta agtatgaata taccgtcatg ccatttggct tagtcaatgc acctagtaca
     2881 ttcgcaagat acatggctga tacatttaga gacctgagat tcgtcaatgt ttaccttgat
     2941 gatatattaa tattctccga atctccagaa gaacattgga aacatttaga cacggtacta
     3001 gaaagattaa agaacgagaa cctcattgtt aagaagaaaa aatgtaaatt tgcatctgaa
     3061 gaaactgagt ttttaggcta tagtattgga atccagaaaa tagctccact acagcacaaa
     3121 tgtgcagcaa tccgagactt tccgacgcct aaaacagtaa aacaagcaca gagattttta
     3181 ggaatgatta attactacag acgattcatt ccaaattgct ccaagattgc acagccaatc
     3241 caactgttta tttgtgacaa aagtcaatgg acagaaaaac aagacaaggc aattgataaa
     3301 ctaaaagacg ccttgtgtaa ctcccccgtc ctagtaccat tcaacaacaa agcaaactac
     3361 cgacttacaa cagacgcctc aaaagacggc attggtgctg ttctagaaga agtcgacaac
     3421 aagaacaaac ttgttggtgt cgtcggttac ttctctaaat ccttagagag tgcccagaaa
     3481 aactatcctg ctggcgaatt agaactactt ggaattatca aagcactcca ccacttccga
     3541 tatatgcttc acggaaagca tttcacgtta agaacagacc acattagttt gttatcatta
     3601 caaaacaaga acgaacccgc acgacgcgtg caacgctggt tagatgacct agccacatat
     3661 gacttcacct tagaatacct agctggaccc aagaacgttg tcgcagatgc catatcccgt
     3721 gccgtatata ctataacccc cgaaacatcc cgacctatcg acacagaaag ctggaaatct
     3781 tactacaaat cagacccatt atgtagtgct gtcttaattc atatgaaaga attgacacaa
     3841 cacaacgtca cacctgaaga tatgtcagcc ttccgtagtt accagaagaa actcgaacta
     3901 tcagagacct tccgaaagaa ttattcccta gaagacgaaa tgatctatta ccaagaccga
     3961 ctagtagtac caataaaaca acagaacgca gttatgagac tatatcatga ccatacctta
     4021 tttggaggac attttggtgt aacagtgacc cttgcgaaaa tcagcccaat ttactattgg
     4081 ccaaaattac aacattcgat catacaatac atcaggacct gcgtacaatg tcaactaata
     4141 aaatcacacc gaccacgctt acatggacta ttacaaccac tccctatagc agaaggaaga
     4201 tggcttgata tatcaatgga ttttgtgaca ggattacccc cgacatcaaa taacttgaat
     4261 atgatcctcg tcgtagttga tcgtttttcg aaacgcgctc acttcatagc tacaaggaaa
     4321 accttagacg caacacaact aatagatcta ctctttcgat acattttttc atatcatggt
     4381 tttcccagga caataaccag tgatagagat gtccgtatga ccgccgacaa atatcaagaa
     4441 ctcacgaaaa gactaggaat aaaatcgaca atgtcttccg cgaaccaccc ccaaacagat
     4501 ggacaatccg aacgaacgat acagacatta aacaggttac taagagccta tgcttcaacc
     4561 aatattcaga attggcatgt atatttacca caaatcgaat ttgtttacaa ttctacacct
     4621 actagaacac ttggaaaatc accatttgaa attgatttag gatatttacc gaatacccct
     4681 gctattaagt cagatgacga agtcaacgca agaagtttta ctgccgtaga acttgccaaa
     4741 cacctcaaag cccttaccat ccaaacgaag gaacagctag aacacgctca aatcgaaatg
     4801 gaaactaata acaatcaaag acgtaaaccc ttattgttaa acataggaga tcacgtatta
     4861 gtgcatagag atgcatactt caagaaaggt gcttatatga aagtacaaca aatatacgtc
     4921 ggaccatttc gagttgtcaa gaaaataaac gataacgcct acgaactaga tttaaactct
     4981 cacaagaaaa agcacagagt tattaatgta caattcctga aaaagtttgt ataccgtcca
     5041 gacgcgtacc caaagaataa accaatcagc tccactgaaa gaattaagag agcacacgaa
     5101 gttactgcac tcataggaat agatactaca cacaaaactt acttatgtca catgcaagat
     5161 gtagacccaa cactttcagt agaatactca gaagctgaat tttgccaaat tcccgaaaga
     5221 acacgaagat caatattagc caactttaga caactctacg aaacacaaga caaccctgag
     5281 agagaggaag atgttgtatc tcaaaatgag atatgtcagt atgacaatac gtcaccctaa
     5341 taagagctct cctagtcgca cgcttgtagt ggatcgtctg cataatatcg tgtgacccgg
     5401 gtgagagaga ggaagatgtt gtatctcaaa atgagatatg tcagtatgac aatacgtcac
     5461 cctgaacgtt cataaaacac atatgaaaca accttataac aaaacgaaca acatgagaca
     5521 aaacccgacc ttccctagct gaactaccca aagtataaat gcctgaacaa ttagtttaga
     5581 tccgagattc cgcgcttcca ccacttagta tgattcatat tttatataat atataagata
     5641 agtaacattc cgtgaattaa tctgataaac tgttttgaca actggttact tccctaagac
     5701 tgtttatatt aggattgtca agacactccg gtattactcg agcccgtaat acaacaggcg
     5761 gccgcgaatt cctttctgca tgccacgttc gcggcgcctg atgcggtatt ttctccttac
     5821 gcatctgtgc ggtatttcac accgcataat cgctgggcca ttctcatgaa gaatatcttg
     5881 aatttattgt catattacta gttggtgtgg aagtcctaat atcggtgatc aatatagtgg
     5941 ttgacatgct ggctagtcaa cattgagcct tttgatcatg caaatatatt acggtatttt
     6001 acaatcaaat atcaaactta actattgact ttataactta tttaggtggt aacattctta
     6061 taaaaaagaa aaaaattact gcaaaacagt actagctttt aacttgtatc ctaggttatc
     6121 tatgctgtct caccatagag aatattacct atttcagaat gtatgtccat gattcgccgg
     6181 gtaaatacat ataatacaca aatctggctt aataaagtct ataatatatc tcataaagaa
     6241 gtgctaaatt ggctagtgct atatattttt aagaaaattt cttttgacta agtccatatc
     6301 gactttgtaa aagttcacat tagcatacat atattacacg agccagaaat agtaactttt
     6361 gcctaaatca caaattgcaa aatttaattg cttgcaaaag gtcacatgct tataatcaac
     6421 ttttttaaaa atttaaaata cttttttatt ttttattttt aaacataaat gaaataattt
     6481 atttattgtt tatgattacc gaaacataaa acctgctcaa gaaaaagaaa ctgttttgtc
     6541 cttggaaaaa aagcactacc taggagcggc caaaatgccg aggctttcat agcttaaact
     6601 ctttacagaa aataggcatt atagatcagt tcgagttttc ttattcttcc ttccggtttt
     6661 atcgtcacag ttttacagta aataagtatc acctcttaga gttcgatgat aagctgtcaa
     6721 acatgagaat taattccaca tgttaaaata gtgaaggagc atgttcggca cacagtggac
     6781 cgaacgtggg gtaagtgcac tagggtccgg ttaaacggat ctcgcattga tgaggcaacg
     6841 ctaattatca acatatagat tgttatctat ctgcatgaac acgaaatctt tacttgacga
     6901 cttgaggctg atggtgttta tgcaaagaaa ccactgtgtt taatatgtgt cactgtttga
     6961 tattactgtc agcgtagaag ataatagtaa aagcggttaa taagtgtatt tgagataagt
     7021 gtgataaagt ttttacagcg aaaagacgat aaatacaaga aaatgattac gaggatacgg
     7081 agagaggtat gtacatgtgt atttatatac taagctgccg gcggttgttt gcaagaccga
     7141 gaaaaggcta gcaagaatcg ggtcattgta gcgtatgcgc ctgtgaacat tctcttcaac
     7201 aagtttgatt ccattgcggt gaaatggtaa aagtcaaccc cctgcgatgt atattttcct
     7261 gtacaatcaa tcaaaaagcc aaatgattta gcattatctt tacatcttgt tattttacag
     7321 attttatgtt tagatctttt atgcttgctt ttcaaaaggc ttgcaggcaa gtgcacaaac
     7381 aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg acgaaatttg
     7441 ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca tcaacaccaa
     7501 taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca ccagctaaca
     7561 taaaatgtaa gctctcgggg ctctcttgcc ttccaaccca gtcagaaatc gagttccaat
     7621 ccaaaagttc acctgtccca cctgcttctg aatcaaacaa gggaataaac gaatgaggtt
     7681 tctgtgaagc tgcactgagt agtatgttgc agtcttttgg aaatacgagt cttttaataa
     7741 ctggcaaacc gaggaactct tggtattctt gccacgactc atctccatgc agttggacga
     7801 tcgatgataa gctgtcaaac atgagaattg ggtaataact gatataatta aattgaagct
     7861 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     7921 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     7981 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
     8041 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     8101 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     8161 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
     8221 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
     8281 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
     8341 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
     8401 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     8461 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     8521 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     8581 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     8641 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     8701 gctttcgaca tgatttatct tcgtttcctg catgtttttg ttctgtgcag ttgggttaag
     8761 aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     8821 ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     8881 ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag ctaattcttg
     8941 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     9001 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     9061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    10021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10921 gtcagtgagc gaggaagcgg aaga
//
