LOCUS       sequence-218635-       11784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Oct4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11784)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RIX-F2Oct4F3-GFP
FEATURES             Location/Qualifiers
     source          1..11784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..216
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    277..865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(330..350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(748..759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1246..1279)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             1299..1479
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1531..1652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1568..1587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1622..1641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1692..1827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1848..1866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1876..1893)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1876..1892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1885..1907)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2034..2489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2121..2140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2331..2352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2515..2619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2620..2688,2689..3480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2838..2857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3651..4239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4140..4159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4393..4410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4527..4548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4563..4568,4569..4586,4587..4593)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4601..4617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4606..4628
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4625..4641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4625..4641
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4660..4680
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4662..4680
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4708..4934
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4935..5115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5162..5287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5780..6013
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6198..6242
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9835..9952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     9972..9988
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9973..9989
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      11320..11329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11326..11328,11329..11331,11332..11784,1..261)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11326..11328,11329..11331,11332..11784,1..258)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11371..11392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11632..11651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
       61 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      121 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      181 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      241 atggacgagc tgtacaagta aagcggccgc gtcgacaatc aacctctgga ttacaaaatt
      301 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
      361 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
      421 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
      481 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
      541 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
      601 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
      661 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
      721 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
      781 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
      841 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
      901 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
      961 taattcactc ccaacgaaga caagatgtga tcctcctaga gggacagccc ccccccaaag
     1021 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     1081 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     1141 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     1201 ctgcagacac ctggggggat acggggaaaa agcttttcta gtggaataac ttcgtatagc
     1261 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     1321 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1381 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1441 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagagc ggccaaactt
     1501 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     1561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     1681 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     1741 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     1801 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     1861 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1921 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1981 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     2041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2101 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2221 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2461 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     2521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4441 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4501 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4561 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4621 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     4681 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     4741 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     4801 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     4861 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     4921 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5101 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     5161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     5221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     5281 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     5341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     5401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     5461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     5521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     5581 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     5641 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     5701 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     5761 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     5821 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     5881 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     5941 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     6001 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     6061 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     6121 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     6181 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     6241 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     6301 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     6361 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     6421 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     6481 tgaacggatc tcgacggtat cggttgatcc cattactggc ctggtgctta gttatctttg
     6541 caagagaata gccctcagag tgcctgtctg caagggatgg tgtttgtctg tctgctccta
     6601 caccatgctc tgaacgaagg ccccagggtg aatcacacag cctcaaaaag cccagagcac
     6661 cccacagtgt acatagccag gacagagttc tgagcctggg cccagcccgc ctgagctgtt
     6721 ggctagggtc agtggctcta ggtgccctgg ggggcggggg tgtctcttga tggtagtctt
     6781 catcaggtag agatggaacc tttctctata aaacaagctg cccttgaact cctgataatt
     6841 ctcctgcctc agcttcatcg acttcaccaa attcaccctg agactttgaa aaaaaaattg
     6901 cgttttattt taagggtgtg tatgtaccta cacacagcta cacatagcac atgtgtggaa
     6961 gtcccctcct tctaccacat gggtccaagt catcccccag ggtggctgag gggccatctc
     7021 tctggccctc tccatgaatc ttccgtttcc tccactctgt catgctcacc tcccaatttc
     7081 tatacattca ttgtggaaca gtgccatagg ttagttggaa gacctaaata aaggcagcga
     7141 cttggaagcc gccaagttca caaagcttcc tcaatagcag attaaggaag ggctaggacg
     7201 agagggaccc ctccccaacc atcttctctg cccccagggc agagctatca tgcacctttg
     7261 ttatgcatct gccgtctgcc cagaactctc aacctccctg gggggagggg cagggtcggg
     7321 ctacaacctc cccacaccca gttcctccca gggccagcct ctaaggccta gacagcactc
     7381 tccaccacag ttcaagtatg cctgcagccc agcagtcctg tctgtattca ataccaacct
     7441 tgtcttatgg attgtgattt ctcttttggt gactcactgg ccaggacaag agacatattc
     7501 tgagtccttt aactgcctag tgactggctt tgcaaccagg ttagccctaa gcgtgcctag
     7561 agtataatac agtccttaac agcaactttg tctgaagtcc caagtcttct tagaactagc
     7621 ttgaaacaga tgtgccctag cctggtcccg agctgtgagc ctggtggccc tggagatggg
     7681 acagcagacc tgttggctca tctgatccag tttcttgcct cctgggtctt agaaaatcat
     7741 cagactaact tttgggtttg attcaagggt tccctacata gccttagatg gcttggaact
     7801 cactctgtag accaggctgg ccttgaactc acagagatcc accagccttt gcttcctgag
     7861 tgctaggatt gaaaatatgt gccactctgc cccctttaag agtcactttg ggccgggtgc
     7921 ggtcccaact catggcttta atcccagcac ttgggaggca gaggcaggtg gatttctgag
     7981 ttccaggcca gcctggtcta cagagtgagt tccaggacag ccagggcttc acagagaaat
     8041 cctgtctcga aaaaaaaaaa aaatctgtca ccctcttctg gtgtctgaag tacttacgta
     8101 caataataaa taaatctttt taaaaaaaaa aaagaaagaa agaaaaacaa acaaaaaaga
     8161 gtcaccatgg tatcttaaat accccaaatt tgagctaaaa ttcgttaact tgccacaaac
     8221 cacctgtatt ttagaaccaa gtcaagatct gtctcggagt tctgtctgga gaaccaaaga
     8281 cagcagagaa gcccacacgt ccccagccag agatggtcca ccagcatgaa aaggtatcct
     8341 gggaagccag atgcggaatg ccacacctat aatgcctgcc cttgggcgtt ggaagtgata
     8401 agaacaaagg tccaaggcta ggggctggga aagaactcag catttgagtg tacattgctc
     8461 tttcagagga cccttttaaa attctgttcc tagcacccat attgggtggt ttacagctag
     8521 gagaacagaa atctcttggc tgctgtggtc atctgcagtt acatggacac acatatatac
     8581 acatgatttt cttttaaaga gaatttaagg ccagccttaa acacaagagt cctccaggcc
     8641 agcttgagcc agcctcagca ggagaccttg tctaaaacaa aacaaacaaa caataggcgt
     8701 tgacccccac agctttctta ctgtgagttc agccacctcc tcacagactg ggtgatcagt
     8761 taaatggact cacggaggac actagactag agcagggaaa tcactcgtgt tagcatccag
     8821 gctgggtctc cctatgaagc catagggaga aatgaggagt ttcagcgaca tgagctcagt
     8881 caggcctgcc ccagccagga acagcttcct tgctggttag catccccctc ccccaacccc
     8941 aacccccagc cgccctggga ccagcaacgt ctcttcccaa atccagatgt tctggaaggg
     9001 aggaggtagt ttctctggct ggcatagatt ccttagctaa ctgcatctcc ctttccttgt
     9061 cttggttttc ttgcctaatc aaaaagactt gcagggggca ggaggggggg ggggggttgg
     9121 agggggagac ttgaggggag gcctgagagg aacctgagga cagctcagca agtaaccaca
     9181 cttgctgagc ctaacgacca tggatttgat ccctggggtc catttcaaag aagcatttga
     9241 tctccagagg ttcgatattg atattgcact cagatacaca cacacacaca atacaataaa
     9301 aattaaaaga aaaaacaaag atctagaagc tgaaggtagg aataggtcac cagcagaaca
     9361 gaaggcttgc ggaatgtgcc tgctcccttt gaaataaatg agttctattt ctcccttttt
     9421 ttttttttct ttctgagaca gggtttctct gtgtagccct ggctgtcctg gaactcactc
     9481 tgtagaccag gctggccttg agctcagaaa tcagcctgcc ctctgcttcc caagtgctgg
     9541 gattataggc attcgccact actgcccgtt tccttttttc ttctgagaca aatttacatg
     9601 tagcagaggc tgcccctaaa ctctacgtag ccaagaaggc ccttgaactt ctgatcctct
     9661 tgccttccca gttctgatga gcagtgtcca aatgcctttt agacttgcag aaatggcttc
     9721 accactacac ccaaggctgc ccctgataac cagaacccca cgacatcact cattcaagat
     9781 aaccagccac cttgatctgc cttgtaggtg gagagtctcc ccgctaggat caacttttaa
     9841 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9901 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca
     9961 cgagactagc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg
    10021 gggatcctca gactgggccc agaaaaccac tctagggaag ttcagggtag gctctctgca
    10081 ccccctcctc ctaatcccgt ctccttagtg tctttccgcc agcacaggaa tgggggaggg
    10141 gtgggtgacg aggatgaaca ccggagtccc tggaggaagg gaagcagggt atctccatct
    10201 gaggctctgt ctttgaggag aggtggagag ctggggaagt cttgtgtgag gggattgggg
    10261 ctcaggaggg ggttggggag caggaagttg tccccagggg agccatcctg gcccattcaa
    10321 gggttgagta cttgtttagg gttagagctg ccccctctgg ggaccaggat tgtccagcca
    10381 aggccattgt cctgccccct tcccccagtc cctcccaggc ccctttgaac ctgaagtcag
    10441 atatttcttc tctctaccca cctcccaccc gttgggtttc tccacccagg aactaggctg
    10501 gaagcctggg atgaggaggt ggggggaggg agaactgaga atcttgagga aagaggcccc
    10561 ggccttaact gtgaggggat ggagcctggg tgcaggtctt atgggggttg gggggtggtt
    10621 agtgtctaat ctaccaacct ggacaacaca agatggaata ctgtgctctg aaaacgcaga
    10681 gccagcactt ctctggggtc tctggggaca tatctggttg gggctcgggg tcccatggtg
    10741 tagagcctct aaactctgga ggactggagg tgcaatggct gtcttgtcct ggccttggac
    10801 atgggctgaa atactgggtt cacccatatc taggactcta gacgggtggg taagcaagaa
    10861 ctgaggagtg gccccagaaa taattggcac acgaacattc aatggatgtt ttaggctctc
    10921 cagaggatgg ctgagtgggc tgtaaggaca ggccgagagg gtgcagtgcc aacaggcttt
    10981 gtggtgcgat ggggcatccg agcaactggt ttgtgaggtg tccggtgacc caaggcaggg
    11041 gtgagaggac cttgaaggtt gaaaatgaag gcctcctggg gtcccgtcct aagggttgtc
    11101 ctgtccagac gtccccaacc tccgtctgga agacacaggc agatagcgct cgcctcagtt
    11161 tctcccaccc ccacagctct gctcctccac ccacccaggg ggcggggcca gaggtcaagg
    11221 ctagagggtg ggattgggga gggagaggtg aaaccgtccc taggtgagcc gtctttccac
    11281 caggcccccg gctcggggtg cccaccttcc ccaccggtcg ccaccatggt gagcaagggc
    11341 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
    11401 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
    11461 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
    11521 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
    11581 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
    11641 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
    11701 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
    11761 tacaacagcc acaacgtcta tatc
//
