LOCUS       sequence-218639-       13471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  galactose-inducible Ty3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Hansen LJ, Chalker DL, Sandmeyer SB
  TITLE     Ty3, a yeast retrotransposon associated with tRNA genes, has 
            homology to animal retroviruses.
  JOURNAL   Mol Cell Biol. 1988 Dec;8(12):5245-56.
  PUBMED    2854194
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1988-12"; volume: "8(12)"; pages: "5245-56"
COMMENT     Sequence Label: pDLC201
FEATURES             Location/Qualifiers
     source          1..13471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6523..7865
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7486..7533)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(8214..8233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8215..8233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8254..8270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8254..8270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8267..8289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8278..8294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8302..8308,8309..8326,8327..8332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8347..8368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8485..8502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8656..9244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8736..8755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9415..10206,10207..10275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10038..10057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10276..10380)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10448..10466
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10504..10526)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10626..10645
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      11012..11525
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11099..11118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11309..11330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12063..12085
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12077..12094
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12078..12094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12104..12319
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             12320..13123
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    join(13380..13471,1..26)
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 tcacccgctc ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt
       61 actgaaagtt ccaaagagaa ggttttttta ggctaatcga cctcgactct agaggatccc
      121 cctgaactac ccaaagtata aatgcctgaa caattagttt agatccgaga ttccgcgctt
      181 ccaccactta gtatgattca tattttatat aatatataag ataagtaaca ttccgtgaat
      241 taatctgata aactgttttg acaactggtt acttccctaa gactgtttat attaggattg
      301 tcaagacact ccggtattac tcgagcccgt aatacaacac ctggtagcgt taaaggttac
      361 taattgttca aacgaaccat cgaaaagccg aacctagcta caccacaccc cagtatgagc
      421 tttatggatc aaatcccagg aggaggaaat tatccaaaac tcccagtaga atgccttcct
      481 aacttcccga tccaaccatc tttgaccttc agaggtagaa atgactcgca taaactgaaa
      541 aactttatct ccgaaataat gttaaacatg tctatgatat cttggccgaa tgatgccagt
      601 cgtattgtgt actgcagaag acatttatta aaccccgctg ctcagtgggc taatgacttt
      661 gtacaagaac aaggtatact tgaaataaca ttcgacacat tcatacaagg attatatcag
      721 catttctata agccaccaga tatcaataaa atctttaatg caatcacgca actttccgaa
      781 gctaaacttg gtattgagcg tctcaaccaa cgattcagaa agatttggga cagaatgcca
      841 ccagacttca tgaccgaaaa agctgccata atgacatata ctaggctatt gacaaaggaa
      901 acctataata ttgtcagaat gcacaaacca gagacattaa aagacgccat ggaagaggct
      961 taccagacaa ctgcactaac tgaaagattc ttcccaggat tcgaacttga tgctgatgga
     1021 gacactatca tcggtgccac aacccactta caagaagaat acgactctga ctatgattca
     1081 gaagataatc tgacccagaa tggatacgtc cataccgtaa ggacaagaag atcttacaat
     1141 aaaccaatgt caaatcatcg aaacaggaga aataacaacc catctagaga agaatgtata
     1201 aaaaatcggc tatgcttcta ttgtaagaaa gagggacatc gcctgaacga atgtagagca
     1261 cgtaaggcga gttctaaccg atcttgaact cgaatcaaaa gaccaacaaa ctccttttat
     1321 caaaacctta ccaattgtac actatatcgc catccccgag atggacaata ccgccgaaaa
     1381 aaccataaaa atacaaaaca cgaaagtaaa aaccctgttt gacagtggat cacccacgtc
     1441 atttatccga agagatattg tagaacttct caaatacgaa atctacgaga cccctccact
     1501 ccgttttaga ggattcgtag ccaccaaatc cgccgttaca tccgaagcag tcaccattga
     1561 cctcaaaatc aatgacctgc atataacttt agccgcgtac atactggata acatggacta
     1621 ccaattgtta attggaaatc caatcttacg ccgctacccg aaaatcctgc acacagtact
     1681 gaataccaga gagagccccg actccttaaa gcccaagact tatcgctccg aaaccgttaa
     1741 taacgttaga acctactccg ctggtaatcg tggtaacccc agaaacataa aactgtcttt
     1801 tgcccccacc attctcgaag caactgaccc gaaatccgct ggtaatcgtg gtgactccag
     1861 aaccaaaacc ctgtctcttg caaccactac tcctgcagca attgacccgc ttacgaccct
     1921 tgataaccca ggtagtactc aaagtacatt tgcgcaattc ccgatacctg aagaagcgag
     1981 catcctagaa gaggatggaa aatactccaa cgttgtctca accattcaga gtgtagaacc
     2041 taatgctact gatcacagca ataaggacac cttttgcact ttgccagttt ggttacaaca
     2101 gaagtataga gagatcatac gtaatgatct cccaccaaga cctgccgaca ttaataacat
     2161 ccccgtaaaa catgatattg aaattaaacc tggcgcaaga ctacctcgac tacagccata
     2221 ccatgttaca gaaaagaacg aacaagaaat caacaaaata gttcaaaaac tgctcgataa
     2281 caagttcatt gttccctcaa agtcgccttg cagctcccct gtagtcctcg tcccgaagaa
     2341 agacggtacc ttccgactct gcgtcgatta ccgcaccctg aacaaagcta ccatctccga
     2401 cccattccca ttacccagaa tcgacaacct attgagccgt attggaaatg cccagatatt
     2461 taccacgcta gatttgcata gtggttacca ccagatcccg atggaaccca aagaccgcta
     2521 caaaaccgcc tttgtcacac catccggtaa gtatgaatat accgtcatgc catttggctt
     2581 agtcaatgca cctagtacat tcgcaagata catggctgat acatttagag acctgagatt
     2641 cgtcaatgtt taccttgatg atatattaat attctccgaa tctccagaag aacattggaa
     2701 acatttagac acggtactag aaagattaaa gaacgagaac ctcattgtta agaagaaaaa
     2761 atgtaaattt gcatctgaag aaactgagtt tttaggctat agtattggaa tccagaaaat
     2821 agctccacta cagcacaaat gtgcagcaat ccgagacttt ccgacgccta aaacagtaaa
     2881 acaagcacag agatttttag gaatgattaa ttactacaga cgattcattc caaattgctc
     2941 caagattgca cagccaatcc aactgtttat ttgtgacaaa agtcaatgga cagaaaaaca
     3001 agacaaggca attgataaac taaaagacgc cttgtgtaac tcccccgtcc tagtaccatt
     3061 caacaacaaa gcaaactacc gacttacaac agacgcctca aaagacggca ttggtgctgt
     3121 tctagaagaa gtcgacaaca agaacaaact tgttggtgtc gtcggttact tctctaaatc
     3181 cttagagagt gcccagaaaa actatcctgc tggcgaatta gaactacttg gaattatcaa
     3241 agcactccac cacttccgat atatgcttca cggaaagcat ttcacgttaa gaacagacca
     3301 cattagtttg ttatcattac aaaacaagaa cgaacccgca cgacgcgtgc aacgctggtt
     3361 agatgaccta gccacatatg acttcacctt agaataccta gctggaccca agaacgttgt
     3421 cgcagatgcc atatcccgtg ccgtatatac tataaccccc gaaacatccc gacctatcga
     3481 cacagaaagc tggaaatctt actacaaatc agacccatta tgtagtgctg tcttaattca
     3541 tatgaaagaa ttgacacaac acaacgtcac acctgaagat atgtcagcct tccgtagtta
     3601 ccagaagaaa ctcgaactat cagagacctt ccgaaagaat tattccctag aagacgaaat
     3661 gatctattac caagaccgac tagtagtacc aataaaacaa cagaacgcag ttatgagact
     3721 atatcatgac cataccttat ttggaggaca ttttggtgta acagtgaccc ttgcgaaaat
     3781 cagcccaatt tactattggc caaaattaca acattcgatc atacaataca tcaggacctg
     3841 cgtacaatgt caactaataa aatcacaccg accacgctta catggactat tacaaccact
     3901 ccctatagca gaaggaagat ggcttgatat atcaatggat tttgtgacag gattaccccc
     3961 gacatcaaat aacttgaata tgatcctcgt cgtagttgat cgtttttcga aacgcgctca
     4021 cttcatagct acaaggaaaa ccttagacgc aacacaacta atagatctac tctttcgata
     4081 cattttttca tatcatggtt ttcccaggac aataaccagt gatagagatg tccgtatgac
     4141 cgccgacaaa tatcaagaac tcacgaaaag actaggaata aaatcgacaa tgtcttccgc
     4201 gaaccacccc caaacagatg gacaatccga acgaacgata cagacattaa acaggttact
     4261 aagagcctat gcttcaacca atattcagaa ttggcatgta tatttaccac aaatcgaatt
     4321 tgtttacaat tctacaccta ctagaacact tggaaaatca ccatttgaaa ttgatttagg
     4381 atatttaccg aatacccctg ctattaagtc agatgacgaa gtcaacgcaa gaagttttac
     4441 tgccgtagaa cttgccaaac acctcaaagc ccttaccatc caaacgaagg aacagctaga
     4501 acacgctcaa atcgaaatgg aaactaataa caatcaaaga cgtaaaccct tattgttaaa
     4561 cataggagat cacgtattag tgcatagaga tgcatacttc aagaaaggtg cttatatgaa
     4621 agtacaacaa atatacgtcg gaccatttcg agttgtcaag aaaataaacg ataacgccta
     4681 cgaactagat ttaaactctc acaagaaaaa gcacagagtt attaatgtac aattcctgaa
     4741 aaagtttgta taccgtccag acgcgtaccc aaagaataaa ccaatcagct ccactgaaag
     4801 aattaagaga gcacacgaag ttactgcact cataggaata gatactacac acaaaactta
     4861 cttatgtcac atgcaagatg tagacccaac actttcagta gaatactcag aagctgaatt
     4921 ttgccaaatt cccgaaagaa cacgaagatc aatattagcc aactttagac aactctacga
     4981 aacacaagac aaccctgaga gagaggaaga tgttgtatct caaaatgaga tatgtcagta
     5041 tgacaatacg tcaccctgaa cgttcataaa acacatatga aacaacctta taacaaaacg
     5101 aacaacatga gacaaaaccc gaccttccct agctgaacta cccaaagtat aaatgcctga
     5161 acaattagtt tagatccgag attccgcgct tccaccactt agtatgattc atattttata
     5221 taatatataa gataagtaac attccgtgaa ttaatctgat aaactgtttt gacaactggt
     5281 tacttcccta agactgttta tattaggatt gtcaagacac tccggtatta ctcgagcccg
     5341 taatacaaca gaaagttcca ttttggatgc tctatttatg ggaatatgac tatacgaatg
     5401 tcacccacga tggccggagt agctgtgatg agcatctttt tgtcctgggc ggcttaaaac
     5461 gtgagcaaaa ttgtactgta aattacttac gttatcaatg gaagtgtgtg aatttcgtga
     5521 taaaattatt gtcagaatag tgacttcatt attctttata actaaccatt aggatcatat
     5581 aagtaatcag atgcagttcg tcattaatat gtcaggatgg ataaccatag gtaagcacgt
     5641 gattatggaa ggacagtaaa gttaccgatc tacttttttg aatgatcaac ttggtgtttt
     5701 catctataag gatatgggtc gttacattga aactatagta aaagatctcg ttattggtat
     5761 atcggtcata atgaagatga aatagtcggt atgtattttt ataccccccc ctatatggta
     5821 aaaaggttaa gattcatatt taaatcttca ttaataccac ctctaatttc taattaccaa
     5881 catttttaac tccactttta ttatatgcta tatacaacat tatgtaaaag gagtatacca
     5941 aaaattctcc ttcataatat aggaattcca cggactatag actatactag tatactccgt
     6001 ctactgtacg atacacttcc gctcaggtcc ttgtccttta acgaggcctt accactcttt
     6061 tgttactcta ttgatccagc tcagcaaagg cagtgtgatc taagattcta tcttcgcgat
     6121 gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact tctacaatgg
     6181 ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg aatacgcttt
     6241 gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg tacttgttac
     6301 ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga tagtatattt
     6361 gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg tatttcggtt
     6421 cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc cccggttcat
     6481 tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc atctgtgctt
     6541 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     6601 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
     6661 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     6721 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
     6781 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
     6841 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
     6901 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
     6961 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
     7021 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
     7081 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
     7141 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
     7201 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
     7261 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
     7321 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
     7381 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
     7441 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     7501 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     7561 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     7621 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     7681 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     7741 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     7801 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     7861 ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat tgctgttatc
     7921 tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca atttcccaca
     7981 acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca
     8041 atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt tttcttcaaa
     8101 gctttattgt acgatctgac taagttatct tttaataatt ggtattcctg tttattgctt
     8161 gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcgg tctccctata
     8221 gtgagtcgta ttaattcgat atccgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10381 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10441 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10501 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10561 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10621 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10681 cagatgcgta aggagaaaat accgcatcag gcgattccgt tgcaatggct ggcggtaata
    10741 ttgttctgga tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg
    10801 ttattactaa tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt
    10861 tactcggtgg cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt
    10921 ctaaaatccc tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca
    10981 cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
    11041 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    11101 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    11161 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    11221 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    11281 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    11341 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    11401 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    11461 acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc
    11521 ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc
    11581 tccagactct caggcaatga cctgatagcc tttgtagacc tctcaaaaat agctaccctc
    11641 tccggcatga atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc
    11701 tccggccttt ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa
    11761 atatatgagg gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa
    11821 gtattacagg gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta
    11881 ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt tattggatgt tggaacgcca
    11941 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    12001 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    12061 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttttcaatt catcattttt
    12121 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
    12181 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
    12241 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
    12301 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
    12361 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
    12421 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
    12481 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
    12541 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
    12601 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
    12661 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
    12721 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
    12781 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
    12841 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
    12901 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
    12961 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
    13021 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
    13081 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
    13141 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
    13201 cgatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc
    13261 gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt ggggccaggt
    13321 tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc
    13381 ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agacgaggac
    13441 gcacggagga gagtcttcct tcggagggct g
//
