LOCUS       sequence-218640-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone containing mCherry-STIM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, 
            Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, 
            Martinvalet D, Manoury B, Demaurex N
  TITLE     STIM1 promotes migration, phagosomal maturation and antigen 
            cross-presentation in dendritic cells.
  JOURNAL   Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.
  PUBMED    29176619
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-24"; volume: "8(1):1852. doi"; pages: " 
            10.1038/s41467-017-01600-6"
COMMENT     Sequence Label: pENTR1a-mCherry-STIM1
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             583..1290
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(728..746)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     996..1015
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3267..3366)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3478..3497)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3489..4298
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3556..3575)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4388..4976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4877..4896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atggatgtat gcgtccgtct tgccctgtgg ctcctctggg gactcctcct gcaccagggc
      541 cagagcctca gccatagtca cagtgagaag gcgacaggta ccatggtgag caagggcgag
      601 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      661 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      721 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      781 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      841 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      901 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      961 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1021 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1081 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1141 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1201 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1261 tccaccggcg gcatggacga gctgtacaag ggtaccagct cgggggccaa ctctgaggag
     1321 tccactgcag cagagttttg ccgaattgac aagcccctgt gtcacagtga ggatgagaaa
     1381 ctcagcttcg aggcagtccg taacatccac aaactgatgg acgatgatgc caatggtgat
     1441 gtggatgtgg aagaaagtga tgagttcctg agggaagacc tcaattacca tgacccaaca
     1501 gtgaaacaca gcaccttcca tggtgaggat aagctcatca gcgtggagga cctgtggaag
     1561 gcatggaagt catcagaagt atacaattgg accgtggatg aggtggtaca gtggctgatc
     1621 acatatgtgg agctgcctca gtatgaggag accttccgga agctgcagct cagtggccat
     1681 gccatgccaa ggctggctgt caccaacacc accatgacag ggactgtgct gaagatgaca
     1741 gaccggagtc atcggcagaa gctgcagctg aaggctctgg atacagtgct ctttgggcct
     1801 cctctcttga ctcgccataa tcacctcaag gacttcatgc tggtggtgtc tatcgttatt
     1861 ggtgtgggcg gctgctggtt tgcctatatc cagaaccgtt actccaagga gcacatgaag
     1921 aagatgatga aggacttgga ggggttacac cgagctgagc agagtctgca tgaccttcag
     1981 gaaaggctgc acaaggccca ggaggagcac cgcacagtgg aggtggagaa ggtccatctg
     2041 gaaaagaagc tgcgcgatga gatcaacctt gctaagcagg aagcccagcg gctgaaggag
     2101 ctgcgggagg gtactgagaa tgagcggagc cgccaaaaat atgctgagga ggagttggag
     2161 caggttcggg aggccttgag gaaagcagag aaggagctag aatctcacag ctcatggtat
     2221 gctccagagg cccttcagaa gtggctgcag ctgacacatg aggtggaggt gcaatattac
     2281 aacatcaaga agcaaaatgc tgagaagcag ctgctggtgg ccaaggaggg ggctgagaag
     2341 ataaaaaaga agagaaacac actctttggc accttccacg tggcccacag ctcttccctg
     2401 gatgatgtag atcataaaat tctaacagct aagcaagcac tgagcgaggt gacagcagca
     2461 ttgcgggagc gcctgcaccg ctggcaacag atcgagatcc tctgtggctt ccagattgtc
     2521 aacaaccctg gcatccactc actggtggct gccctcaaca tagaccccag ctggatgggc
     2581 agtacacgcc ccaaccctgc tcacttcatc atgactgacg acgtggatga catggatgag
     2641 gagattgtgt ctcccttgtc catgcagtcc cctagcctgc agagcagtgt tcggcagcgc
     2701 ctgacggagc cacagcatgg cctgggatct cagagggatt tgacccattc cgattcggag
     2761 tcctccctcc acatgagtga ccgccagcgt gtggccccca aacctcctca gatgagccgt
     2821 gctgcagacg aggctctcaa tgccatgact tccaatggca gccaccggct gatcgagggg
     2881 gtccacccag ggtctctggt ggagaaactg cctgacagcc ctgccctggc caagaaggca
     2941 ttactggcgc tgaaccatgg gctggacaag gcccacagcc tgatggagct gagcccctca
     3001 gccccacctg gtggctctcc acatttggat tcttcccgtt ctcacagccc cagctcccca
     3061 gacccagaca caccatctcc agttggggac agccgagccc tgcaagccag ccgaaacaca
     3121 cgcattcccc acctggctgg caagaaggct gtggctgagg aggataatgg ctctattggc
     3181 gaggaaacag actccagccc aggccggaag aagtttcccc tcaaaatctt taagaagcct
     3241 cttaagaagt agctcgagat atctagaccc agctttcttg tacaaagttg gcattataag
     3301 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3361 tatttgccat ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     3421 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     3481 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     3541 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     3601 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     3661 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     3721 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3781 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     3841 ttgttgatgc gctggcagtg tccctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     3901 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     3961 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     4021 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4081 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4141 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     4201 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4261 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     4321 aacattattc agattgggcc ccgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4381 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4441 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4501 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4561 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4621 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4681 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4741 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4801 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4861 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4921 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4981 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
