LOCUS       sequence-218653-        7809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in of P2A-eGFP at the mouse Sox1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Deluz C, Friman ET, Strebinger D, Benke A, Raccaud M, Callegari A, 
            Leleu M, Manley S, Suter DM
  TITLE     A role for mitotic bookmarking of SOX2 in pluripotency and 
            differentiation.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 
            10.1101/gad.289256.116. Epub 2016 Dec 5.
  PUBMED    27920086
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.289256.116"; journalName: "Genes Dev"; date: 
            "2016-11-15- 15"; volume: "30"; issue: "22"; pages: "2538-2550"
COMMENT     Sequence Label: pKISox1-P2A-eGFP-Hygro
FEATURES             Location/Qualifiers
     source          1..7809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1085..1141
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1148..1150,1151..1864)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1148..1150,1151..1861)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1190..1211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1451..1470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1798..1819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1871..1904)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1926..2436
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2355..2373
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2451..3476
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2451..3476
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3618..3699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3655..3674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3709..3728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3744..3777)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4821..5276)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4958..4979)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5170..5189
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5303..5407
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5409..5766
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5409..5600
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5432..5452)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5617..5752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5801..6595
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5801..6595
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5855..5874)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6465..6484
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6783..6802)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6827..6874
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7203..7791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7692..7711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtgaattcg agacggtcga
       61 cgctagcttc atggtgtggt cccgcggaca gcggcgcaag atggcccagg aaaaccccaa
      121 gatgcacaac tcggagatca gcaagcgcct cggggccgag tggaaggtca tgtccgaggc
      181 cgagaagcgg ccgttcatcg acgaggccaa gagactgcgc gcgctgcaca tgaaggaaca
      241 cccggattac aagtaccggc cgcgccgcaa gaccaagacg ctgctcaaga aggacaagta
      301 ctcgctggcc ggcgggctgc tagcggccgg cgcgggtggc ggcggcgcgg ccgtggccat
      361 gggtgtgggc gtgggcgtcg gggcggcggc ggtgggccag cgcttggaga gcccaggcgg
      421 cgcggcggga ggaggctacg cgcatgtcaa cggctgggct aacggcgcct accccggctc
      481 ggtggccgcg gcggctgcgg ccgcggccat gatgcaggag gcacagctgg cctacgggca
      541 gcacccaggc gcgggcggcg cgcacccgca cgcacacccg gcgcacccgc acccgcacca
      601 tccgcacgcg catcctcaca acccgcagcc catgcaccgc tacgacatgg gcgcgctgca
      661 gtacagcccc atctccaact ctcagggcta catgagcgcg tcgccttcgg gctacggcgg
      721 catcccttac ggcgccgcgg ccgccgccgc cgccgctgcg ggcggcgcgc accagaactc
      781 ggcggtggcg gcagcggcag ccgcggcagc cgcgtcgtcg ggggccctgg gcgccctcgg
      841 atctctggtc aagtcggagc cgagcggcag tccgccggcc ccggctcact cacgggcacc
      901 gtgtcccggg gacctgcgcg agatgatcag catgtacctg cccgccggcg agggtggcga
      961 cccggcggcg gcagcggctg cggcggccca aagccggctg cactcgctgc cacagcacta
     1021 ccagggcgcg ggcgcgggcg tcaacggcac ggtgcccctg acgcacatcg atatcggaag
     1081 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc
     1141 taacgttgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1201 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1261 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1321 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1381 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1441 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1501 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1561 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1621 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1681 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1741 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1801 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1861 gtaagagctc ataacttcgt atagcataca ttatacgaag ttatatcgat atatcgaatt
     1921 cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     1981 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2041 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     2101 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     2161 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     2221 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     2281 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     2341 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     2401 tccctcgttg accgaatcac cgacctctct ccccaggggg atccgccacc atgaaaaagc
     2461 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     2521 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     2581 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     2641 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     2701 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     2761 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     2821 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     2881 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     2941 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     3001 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     3061 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3121 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3181 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3241 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3301 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3361 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3421 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagctcg
     3481 agttccggtg aaacaggcgg ccgcgactct agatcataat cagccatacc acatttgtag
     3541 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3601 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3661 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3721 aactcatcaa tgtatctttt aaaataactt cgtatagcat acattatacg aagttattaa
     3781 gctctagaga cactgcggct taaggccggc gccccggcga cgaagagcga ggcctgcgcc
     3841 ccagcctcca gagcccgact ttgtaccgag gtccccgcgc tctcgataaa aggccgctct
     3901 ggagagccga gcgccaggtg acatctgccc ccatcacctt ccccaggact ccgaggcgct
     3961 gacaccagac tggcctctta gactgaactt tggtgttttc atgagacctt ttgtacagta
     4021 tttatcgtcc gcagaggagg cacacagcgt tttctcggct tcggaggaca aaagacaaaa
     4081 acccagcgag gcgatgccaa cttttgtatg actgccggct ctgtaacttt ttccggggtt
     4141 tacttcccgc cagctcttct gcctgaggcc gagtgacgga cctcgagccc ttctcacttg
     4201 ttataaatct aagtaaggca gatccaaaca tttacaagtt ttttgtagtt gttaccgctc
     4261 ttttgggttg gttggttaat ttataccgca atcccctctc agacggtgga gttatattct
     4321 gggttttgta aatctctgta tccgagcatt tccaattttt tgttttgttt tgtattattt
     4381 cttgtaaatg cgttgtgaca tttttatttt aggcgttgcg atacgggggg aagaggagtc
     4441 ggatgttgta catagcctgc aagtctttca tctaaaagca aaaaacaaag agagataccc
     4501 ccaaaatgca tcaaatttga atcaatacat ttaagagaga aaagcccgtt tccctgtgaa
     4561 atcgaaacgt gctaatttta tatgcatgtt ttatgagttc aaaatacaat tagcaatctg
     4621 ttggaggaga aattatctgc agggcccaag agtaaggaaa ccccaccaag cggagtgcag
     4681 cgtttccgtg actttattcg gtggggaaag agaaatctta aactccatag attattttgt
     4741 agagttcaag ttgacacaga agggaagtaa ttacaaaatg cttcctctag actcaggtac
     4801 cgcatatgct taaaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4861 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4921 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4981 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5041 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5101 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5161 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5221 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     5281 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5341 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5401 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     5461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5521 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5581 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5641 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5701 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5761 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5821 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5881 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5941 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     6001 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6061 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     6121 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     6181 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     6241 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     6301 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     6361 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6421 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6481 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6541 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     6601 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6661 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6721 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     6781 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     6841 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     6901 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     6961 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     7021 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     7081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7321 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7801 aacgcggcc
//
