LOCUS       sequence-218667-       11172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of mCherry-STIM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, 
            Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, 
            Martinvalet D, Manoury B, Demaurex N
  TITLE     STIM1 promotes migration, phagosomal maturation and antigen 
            cross-presentation in dendritic cells.
  JOURNAL   Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.
  PUBMED    29176619
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-24"; volume: "8(1):1852. doi"; pages: " 
            10.1038/s41467-017-01600-6"
COMMENT     Sequence Label: p2K7bsdUBI-mCherry-STIM1
FEATURES             Location/Qualifiers
     source          1..11172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1976..3187
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3024..3043
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3194..3218
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3344..4051
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3489..3507)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3757..3776
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6028..6052)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6070..6658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6123..6143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6541..6552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6682..6723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6760..6780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6765..7094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6945..7080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7142..7189
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7208..7606
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7693..7926
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7998..8119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8035..8054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8089..8108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8159..8294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8314..8333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8315..8333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8343..8360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8343..8359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8352..8374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8501..8956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8588..8607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8798..8819
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8982..9086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9087..9155,9156..9947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9305..9324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10118..10706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10607..10626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10860..10877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10994..11015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11030..11035,11036..11053,11054..11060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11068..11084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11073..11095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11092..11108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11092..11108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11127..11147
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11129..11147
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt aattaagggt gcagcggcct
     1981 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     2041 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     2101 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     2161 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     2221 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     2281 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     2341 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctagccg
     2401 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     2461 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     2521 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     2581 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     2641 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     2701 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     2761 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     2821 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     2881 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     2941 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     3001 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     3061 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     3121 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     3181 gttagacagg atcccaactt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
     3241 catggatgta tgcgtccgtc ttgccctgtg gctcctctgg ggactcctcc tgcaccaggg
     3301 ccagagcctc agccatagtc acagtgagaa ggcgacaggt accatggtga gcaagggcga
     3361 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3421 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3481 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3541 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3601 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3661 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3721 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3781 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3841 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3901 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3961 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     4021 ctccaccggc ggcatggacg agctgtacaa gggtaccagc tcgggggcca actctgagga
     4081 gtccactgca gcagagtttt gccgaattga caagcccctg tgtcacagtg aggatgagaa
     4141 actcagcttc gaggcagtcc gtaacatcca caaactgatg gacgatgatg ccaatggtga
     4201 tgtggatgtg gaagaaagtg atgagttcct gagggaagac ctcaattacc atgacccaac
     4261 agtgaaacac agcaccttcc atggtgagga taagctcatc agcgtggagg acctgtggaa
     4321 ggcatggaag tcatcagaag tatacaattg gaccgtggat gaggtggtac agtggctgat
     4381 cacatatgtg gagctgcctc agtatgagga gaccttccgg aagctgcagc tcagtggcca
     4441 tgccatgcca aggctggctg tcaccaacac caccatgaca gggactgtgc tgaagatgac
     4501 agaccggagt catcggcaga agctgcagct gaaggctctg gatacagtgc tctttgggcc
     4561 tcctctcttg actcgccata atcacctcaa ggacttcatg ctggtggtgt ctatcgttat
     4621 tggtgtgggc ggctgctggt ttgcctatat ccagaaccgt tactccaagg agcacatgaa
     4681 gaagatgatg aaggacttgg aggggttaca ccgagctgag cagagtctgc atgaccttca
     4741 ggaaaggctg cacaaggccc aggaggagca ccgcacagtg gaggtggaga aggtccatct
     4801 ggaaaagaag ctgcgcgatg agatcaacct tgctaagcag gaagcccagc ggctgaagga
     4861 gctgcgggag ggtactgaga atgagcggag ccgccaaaaa tatgctgagg aggagttgga
     4921 gcaggttcgg gaggccttga ggaaagcaga gaaggagcta gaatctcaca gctcatggta
     4981 tgctccagag gcccttcaga agtggctgca gctgacacat gaggtggagg tgcaatatta
     5041 caacatcaag aagcaaaatg ctgagaagca gctgctggtg gccaaggagg gggctgagaa
     5101 gataaaaaag aagagaaaca cactctttgg caccttccac gtggcccaca gctcttccct
     5161 ggatgatgta gatcataaaa ttctaacagc taagcaagca ctgagcgagg tgacagcagc
     5221 attgcgggag cgcctgcacc gctggcaaca gatcgagatc ctctgtggct tccagattgt
     5281 caacaaccct ggcatccact cactggtggc tgccctcaac atagacccca gctggatggg
     5341 cagtacacgc cccaaccctg ctcacttcat catgactgac gacgtggatg acatggatga
     5401 ggagattgtg tctcccttgt ccatgcagtc ccctagcctg cagagcagtg ttcggcagcg
     5461 cctgacggag ccacagcatg gcctgggatc tcagagggat ttgacccatt ccgattcgga
     5521 gtcctccctc cacatgagtg accgccagcg tgtggccccc aaacctcctc agatgagccg
     5581 tgctgcagac gaggctctca atgccatgac ttccaatggc agccaccggc tgatcgaggg
     5641 ggtccaccca gggtctctgg tggagaaact gcctgacagc cctgccctgg ccaagaaggc
     5701 attactggcg ctgaaccatg ggctggacaa ggcccacagc ctgatggagc tgagcccctc
     5761 agccccacct ggtggctctc cacatttgga ttcttcccgt tctcacagcc ccagctcccc
     5821 agacccagac acaccatctc cagttgggga cagccgagcc ctgcaagcca gccgaaacac
     5881 acgcattccc cacctggctg gcaagaaggc tgtggctgag gaggataatg gctctattgg
     5941 cgaggaaaca gactccagcc caggccggaa gaagtttccc ctcaaaatct ttaagaagcc
     6001 tcttaagaag tagctcgaga tatctagacc cagctttctt gtacaaagtg gtgataatcg
     6061 aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6661 cgggaattcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     6721 acgcgtaccg gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt
     6781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7021 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7081 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7141 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7201 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     7261 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     7321 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     7381 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     7441 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     7501 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     7561 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct
     7621 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7681 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7741 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7801 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7861 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7921 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7981 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8101 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8161 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8221 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8281 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8341 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8401 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8461 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8941 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9001 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9061 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9121 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9181 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9241 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9301 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9361 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9421 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9481 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9541 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9601 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9661 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9721 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9781 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9841 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9901 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9961 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10021 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10081 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10141 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10201 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10261 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10321 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10381 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10441 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10501 agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg
    10561 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10621 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10681 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10741 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10801 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10861 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10921 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10981 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11041 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11101 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    11161 agctgcaagc tt
//
