LOCUS       sequence-218668-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of tagRFP with a signal peptide and
            KDEL ER retention signal for expression of tagRFP in the ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, 
            Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, 
            Martinvalet D, Manoury B, Demaurex N
  TITLE     STIM1 promotes migration, phagosomal maturation and antigen 
            cross-presentation in dendritic cells.
  JOURNAL   Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.
  PUBMED    29176619
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-24"; volume: "8(1):1852. doi"; pages: " 
            10.1038/s41467-017-01600-6"
COMMENT     Sequence Label: p2K7-bsd-UBI-tagRFP-KDEL
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1976..3187
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3024..3043
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3194..3218
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3335..4066
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    complement(4098..4122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4140..4728
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4193..4213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4611..4622)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4752..4793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4830..4850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4835..5164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5015..5150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5212..5259
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5278..5676
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5763..5996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6068..6189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6105..6124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6159..6178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6229..6364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6384..6403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6385..6403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6413..6430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6413..6429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6422..6444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6571..7026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6658..6677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6868..6889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7052..7156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7157..7225,7226..8017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7375..7394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8188..8776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8677..8696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8930..8947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9064..9085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9100..9105,9106..9123,9124..9130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9138..9154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9143..9165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9162..9178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9162..9178
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9197..9217
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9199..9217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt aattaagggt gcagcggcct
     1981 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     2041 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     2101 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     2161 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     2221 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     2281 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     2341 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctagccg
     2401 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     2461 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     2521 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     2581 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     2641 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     2701 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     2761 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     2821 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     2881 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     2941 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     3001 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     3061 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     3121 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     3181 gttagacagg atcccaactt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
     3241 ctggatccgg taccgaagcc gctagcccgg gatcgccacc atgctgctgc ccgtccccct
     3301 gctgctgggc ctgctgggcg ccgccgccga catcatggtg agcaagggcg aagagctgat
     3361 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     3421 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     3481 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3541 cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3601 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     3661 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     3721 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
     3781 gatgctgtac cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt
     3841 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
     3901 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
     3961 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     4021 tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagaagg acgagctgta
     4081 aatctcgaga tatctagacc cagctttctt gtacaaagtg gtgataatcg aattccgata
     4141 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4201 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4261 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4321 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4381 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4441 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4501 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4561 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4621 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4681 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc
     4741 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     4801 gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4861 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4921 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4981 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5041 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5101 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5161 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     5221 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     5281 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     5341 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     5401 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     5461 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     5521 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5581 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5641 gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt
     5701 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5761 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6001 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6061 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6181 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6361 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6721 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6781 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6901 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6961 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7021 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8581 atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag
     8641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8821 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8881 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8941 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9001 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9061 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9121 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9181 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     9241 tt
//
