LOCUS       sequence-218673-        6290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 684, Emission: 708.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6290)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IFP2.0-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2039..2048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2045..3010
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     polyA_signal    3132..3253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3169..3188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3223..3242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3260..3715)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3397..3418)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3609..3628
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3742..3846
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3848..4205
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3848..4039
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3871..3891)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4056..4191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4240..5034
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4240..5034
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4294..4313)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4904..4923
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5222..5241)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5266..5313
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5642..6230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6131..6150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattcgg cagcactttt ctttcattgg ggggaaggcg
      721 tgaggaaagt accaaacagc agcagacttt taaactttaa acagacaggt ctgagagcct
      781 gaactctcat ttttcctttg acttcagcct ccaaggagtt ccaccaactt tggcgcgccg
      841 gcttcacttt cattaagtga aagagaggtg cccagacatg ggtgactgga gtgccttggg
      901 gaagcttctg gacaaggtcc aagcctactc caccgctgga gggaaggtgt ggctgtcagt
      961 gctcttcata ttcagaatcc tgctcctggg gacagctgtt gagtcagctt ggggtgatga
     1021 acagtctgcc tttcgctgta acactcaaca acctggctgc gaaaacgtct gctatgacaa
     1081 gtccttcccc atctctcacg tgcgcttctg ggtccttcag atcatattcg tgtctgtgcc
     1141 cacactcctg tacttggccc atgtgttcta tgtgatgagg aaggaagaga agctaaacaa
     1201 gaaagaagag gagctcaaag tggcccagac tgacggggtc aacgtggaga tgcacctgaa
     1261 gcagattgaa atcaagaagt tcaagtacgg gattgaagag cacggcaagg tgaaaatgag
     1321 gggcggcttg ctgagaacct acatcatcag catcctcttc aagtctgtct tcgaggtggc
     1381 cttcctgctc atccagtggt acatctatgg gttcagcttg agcgcggtct acacctgcaa
     1441 gagagatccc tgcccccacc aggtagactg cttcctctca cgtcccacgg agaaaaccat
     1501 cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc gctttgaaca tcattgagct
     1561 cttctacgtc ttcttcaagg gcgttaagga tcgcgtgaag ggaagaagcg atccttacca
     1621 cgccaccact ggcccactga gcccatcaaa agactgcgga tctccaaaat acgcctactt
     1681 caatggctgc tcctcaccaa cggctccact ctcgcctatg tctcctcctg ggtacaagct
     1741 ggttactggt gacagaaaca attcctcgtg ccgcaattac aacaagcaag ctagcgagca
     1801 aaactgggcg aactacagcg cagagcaaaa tcgcatgggg caggccggaa gcaccatctc
     1861 caactcgcac gcccagccgt tcgatttccc cgacgacaac cagaatgcca aaaaagttgc
     1921 tgctggacat gaactccagc cattagccat cgtggaccaa cgaccttcca gcagagccag
     1981 cagccgcgcc agcagcaggc ctcggcctga tgacctggag attgcggatc caccggtcgc
     2041 caccatggct cgggaccctc aacctttctt ccctcctctg tatctcggag gaccagagat
     2101 cactactgag aactgcgagc gggaacctat acatatacca ggttctattc aacctcatgg
     2161 agcactgctc acagccgatg gacattctgg cgaggtgctg caagtttcac tcaacgcagc
     2221 cactttcctg ggccaggaac caactgttct gcggggacag accctggcag ccctgctgcc
     2281 agaccagtgg cctgctctgc agactgccct gcctccaggc tgccaggacg ccctgcagta
     2341 tcgcgccacc ctggattggc ccgccgccgg acacctgagc ctgaccgtgc atagggtggc
     2401 cgagctgctg attctggaat tcgagcctac tgaggcatgg gactctattg gaccccacgc
     2461 tctgagaaat gcaatgttcg ctctggagag tgctcctaat ctccgggcac tggctgaagt
     2521 cgcaacccaa acagtccggg aactgtcagg tttcgaccgg gtgatgctgt acaagtttgc
     2581 accagacgca acaggagagg ttattgccga agcaaggcgc gagggcatgc aggcttacct
     2641 cgggcatagg tttcccgcat ccaccacccc tgcacaagct agggccctct acacaagaca
     2701 cctgctccgg ctgaccgcag acaccagggc tgcagcagtg cccctcgacc ccgtgctgaa
     2761 tccccagaca aatgctccta cacctctggg cggagctgtc ctcagagcta catccccaat
     2821 gcacatgcag tacctgagga atatgggagt gggctcctcc ctgagcgtca gcgtcgtggt
     2881 cggcggccag ctgtggggac tgattgtctg ccaccatcag acaccctacg tgctgccacc
     2941 cgatctgcgg accaccctgg agtatctggg gaggctcctg tccctgcagg tgcagaggaa
     3001 agaagcctga gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     3061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3241 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3301 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3361 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3421 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3481 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     3541 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     3601 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3661 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     3721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3841 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3901 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3961 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4021 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4081 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4141 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4201 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4261 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4321 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4381 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     4441 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4501 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4561 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4621 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4681 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4741 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     4801 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4861 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4921 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4981 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5041 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5101 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5161 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     5221 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     5281 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     5341 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     5401 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     5461 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     5521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
//
