LOCUS       sequence-218678-       12554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mifepristone-inducible expression of hM3Dq protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLX-MIFE-hM3Dq-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..12554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1824..1848)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3933..4304
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     promoter        4335..4845
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4764..4782
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4863..5570
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5005..5023)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5273..5292
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5594..5610)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5595..5611)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5634..6222
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5687..5707)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6105..6116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6364..6397)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6417..6597
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6635..6654)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6700..6755
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6754..6773)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7116..7132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7116..7132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7129..7151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7140..7156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7164..7170,7171..7188,7189..7194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7209..7230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7289..7484
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7335..7470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7490..7624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7527..7546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7581..7600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7692..7709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7863..8451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7943..7962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8622..9413,9414..9482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9245..9264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9483..9587)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9618..9997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9999..10256
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10303..10428
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10920..11037
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11397..11630
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11815..11859
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     12216..12240
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    12493..12517
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tacccatacg atgttccaga ttacgctatg accttgcaca ataacagtac aacctcgcct
       61 ttgtttccaa acatcagctc ctcctggata cacagcccct ccgatgcagg gctgcccccg
      121 ggaaccgtca ctcatttcgg cagctacaat gtttctcgag cagctggcaa tttctcctct
      181 ccagacggta ccaccgatga ccctctggga ggtcataccg tctggcaagt ggtcttcatc
      241 gctttcttaa cgggcatcct ggccttggtg accatcatcg gcaacatcct ggtaattgtg
      301 tcatttaagg tcaacaagca gctgaagacg gtcaacaact acttcctctt aagcctggcc
      361 tgtgccgatc tgattatcgg ggtcatttca atgaatctgt ttacgaccta catcatcatg
      421 aatcgatggg ccttagggaa cttggcctgt gacctctggc ttgccattga ctgcgtagcc
      481 agcaatgcct ctgttatgaa tcttctggtc atcagctttg acagatactt ttccatcacg
      541 aggccgctca cgtaccgagc caaacgaaca acaaagagag ccggtgtgat gatcggtctg
      601 gcttgggtca tctcctttgt cctttgggct cctgccatct tgttctggca atactttgtt
      661 ggaaagagaa ctgtgcctcc gggagagtgc ttcattcagt tcctcagtga gcccaccatt
      721 acttttggca cagccatcgc tggtttttat atgcctgtca ccattatgac tattttatac
      781 tggaggatct ataaggaaac tgaaaagcgt accaaagagc ttgctggcct gcaagcctct
      841 gggacagagg cagagacaga aaactttgtc caccccacgg gcagttctcg aagctgcagc
      901 agttacgaac ttcaacagca aagcatgaaa cgctccaaca ggaggaagta tggccgctgc
      961 cacttctggt tcacaaccaa gagctggaaa cccagctccg agcagatgga ccaagaccac
     1021 agcagcagtg acagttggaa caacaatgat gctgctgcct ccctggagaa ctccgcctcc
     1081 tccgacgagg aggacattgg ctccgagacg agagccatct actccatcgt gctcaagctt
     1141 ccgggtcaca gcaccatcct caactccacc aagttaccct catcggacaa cctgcaggtg
     1201 cctgaggagg agctggggat ggtggacttg gagaggaaag ccgacaagct gcaggcccag
     1261 aagagcgtgg acgatggagg cagttttcca aaaagcttct ccaagcttcc catccagcta
     1321 gagtcagccg tggacacagc taagacttct gacgtcaact cctcagtggg taagagcacg
     1381 gccactctac ctctgtcctt caaggaagcc actctggcca agaggtttgc tctgaagacc
     1441 agaagtcaga tcactaagcg gaaaaggatg tccctggtca aggagaagaa agcggcccag
     1501 accctcagtg cgatcttgct tgccttcatc atcacttgga ccccatacaa catcatggtt
     1561 ctggtgaaca ccttttgtga cagctgcata cccaaaacct tttggaatct gggctactgg
     1621 ctgtgctaca tcaacagcac cgtgaacccc gtgtgctatg ctctgtgcaa caaaacattc
     1681 agaaccactt tcaagatgct gctgctgtgc cagtgtgaca aaaaaaagag gcgcaagcag
     1741 cagtaccagc agagacagtc ggtcattttt cacaagcgcg cacccgagca ggccttgtag
     1801 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggtga ttcgacctgc
     1861 aggtcgaagc ggagtactgt cctccgagtt taaaagcgga gtactgtcct ccgaggatat
     1921 cagcggagta ctgtcctccg agtcgcgaag cggagtactg tcctccgaga tcgatgtcga
     1981 ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     2041 gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaatcgc ctggagacgc
     2101 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     2161 gaacggtgca ttggaacgcg gattccccgt gttaattaac aggtaagtgt cttcctcctg
     2221 tttccttccc ctgctattct gctcaacctt cctatcagaa actgcagtat ctgtattttt
     2281 gctagcagta atactaacgg ttcttttttt ctcttcacag gccaccaagc taccggtcca
     2341 ccatggactc ccagcagcca gatctgaagc tactgtcttc tatcgaacaa gcatgcgata
     2401 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
     2461 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
     2521 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
     2581 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
     2641 tagaattccc gggtgtcgac cagaaaaagt tcaataaagt cagagttgtg agagcactgg
     2701 atgctgttgc tctcccacag ccagtgggcg ttccaaatga aagccaagcc ctaagccaga
     2761 gattcacttt ttcaccaggt caagacatac agttgattcc accactgatc aacctgttaa
     2821 tgagcattga accagatgtg atctatgcag gacatgacaa cacaaaacct gacacctcca
     2881 gttctttgct gacaagtctt aatcaactag gcgagaggca acttctttca gtagtcaagt
     2941 ggtctaaatc attgccaggt tttcgaaact tacatattga tgaccagata actctcattc
     3001 agtattcttg gatgagctta atggtgtttg gtctaggatg gagatcctac aaacacgtca
     3061 gtgggcagat gctgtatttt gcacctgatc taatactaaa tgaacagcgg atgaaagaat
     3121 catcattcta ttcattatgc cttaccatgt ggcagatccc acaggagttt gtcaagcttc
     3181 aagttagcca agaagagttc ctctgtatga aagtattgtt acttcttaat acaattcctt
     3241 tggaagggct acgaagtcaa acccagtttg aggagatgag gtcaagctac attagagagc
     3301 tcatcaaggc aattggtttg aggcaaaaag gagttgtgtc gagctcacag cgtttctatc
     3361 aacttacaaa acttcttgat aacttgcatg atcttgtcaa acaacttcat ctgtactgct
     3421 tgaatacatt tatccagtcc cgggcactga gtgttgaatt tccagaaatg atgtctgaag
     3481 ttattgctgg gtcgacgccc atggaattcc agtacctgcc agatacagac gatcgtcacc
     3541 ggattgagga gaaacgtaaa aggacatatg agaccttcaa gagcatcatg aagaagagtc
     3601 ctttcagcgg acccaccgac ccccggcctc cacctcgacg cattgctgtg ccttcccgca
     3661 gctcagcttc tgtccccaag ccagcacccc agccctatcc ctttacgtca tccctgagca
     3721 ccatcaacta tgatgagttt cccaccatgg tgtttccttc tgggcagatc agccaggcct
     3781 cggccttggc cccggcccct ccccaagtcc tgccccaggc tccagcccct gcccctgctc
     3841 cagccatggt atcagctctg gcccaggccc cagcccctgt cccagtccta gccccaggcc
     3901 ctcctcaggc tgtggcccca cctgccccca agcccaccca ggctggggaa ggaacgctgt
     3961 cagaggccct gctgcagctg cagtttgatg atgaagacct gggggccttg cttggcaaca
     4021 gcacagaccc agctgtgttc acagacctgg catccgtcga caactccgag tttcagcagc
     4081 tgctgaacca gggcatacct gtggcccccc acacaactga gcccatgctg atggagtacc
     4141 ctgaggctat aactcgccta gtgacagggg cccagaggcc ccccgaccca gctcctgctc
     4201 cactgggggc cccggggctc cccaatggcc tcctttcagg agatgaagac ttctcctcca
     4261 ttgcggacat ggacttctca gccctgctga gtcagatcag ctcctaagga tcccccgggc
     4321 tgcaggaatt ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     4381 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     4441 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     4501 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     4561 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     4621 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     4681 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     4741 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     4801 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tggaccggtg
     4861 ccgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     4921 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     4981 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     5041 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     5101 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     5161 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     5221 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     5281 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     5341 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     5401 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     5461 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     5521 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa taggaattcg
     5581 atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacggtc gacaatcaac
     5641 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5701 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5761 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5821 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5881 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5941 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6001 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6061 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6121 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6181 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt
     6241 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6301 ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg
     6361 ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt
     6421 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6481 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6541 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     6601 tctagttgaa tcgccagtgt gtcgagctag cagatctttt tccctctgcc aaaaattatg
     6661 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     6721 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     6781 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     6841 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     6901 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     6961 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     7021 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     7081 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7141 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7201 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7261 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     7321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     7501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7621 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8281 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8521 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8581 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8641 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8701 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8761 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8821 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8881 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8941 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9001 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9061 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9121 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9181 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9241 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9301 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9361 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9421 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9481 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9541 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9601 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     9661 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     9721 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     9781 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     9841 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     9901 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     9961 gcagtacatc tacgtattag tcatcgctat taccatggga ggcgtggcct gggcgggact
    10021 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10261 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    10321 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    10381 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    10441 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    10501 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatactggc
    10561 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    10621 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    10681 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    10741 aaaagtaaga aaaaagcaca gcaagcagca ggatcttcag acctggaaat tccctacaat
    10801 ccccaaagtc aaggagtagt agaatctatg aataaagaat taaagaaaat tataggacag
    10861 gtaagagatc aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat
    10921 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10981 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    11041 gtttattaca gggacagcag aaatccactt tggaaaggac cagcaaagct cctctggaaa
    11101 ggtgaagggg cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa
    11161 gcaaagatca ttagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga
    11221 caggatgagg attagaacat ggaaaagttt agtaaaacac cataaggagg agatatgagg
    11281 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11341 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11401 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11461 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11521 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11581 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11641 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11701 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11761 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11821 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11881 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11941 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12001 tttcagaccc acctcccaac cccgagggga ccccttacgc gggtcgaagc ggagtactgt
    12061 cctccgagtg gagtactgtc ctccgagcgg agtactgtcc tccgagtcga gggtcgaagc
    12121 ggagtactgt cctccgagtg gagtactgtc ctccgagcgg agtactgtcc tccgagtcga
    12181 ctctagaggg tatataatgg atctcgagat atcggagctc gtttagtgaa ccgtcagatc
    12241 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
    12301 ctccgcggcc gggaacggtg cattggaacg cgcattcccc gtgttaatta acaggtaagt
    12361 gtcttcctcc tgtttccttc ccctgctatt ctgctcaacc ttcctatcag aaactgcagt
    12421 atctgtattt ttgctagcag taatactaac ggttcttttt ttctcttcac aggccaccac
    12481 tagagtcgaa tcacaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
    12541 ctggatccac catg
//
