LOCUS       sequence-218680-       19894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate stable Flp-In cells with 
            tetracycline-inducible human PC1 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19894)
  AUTHORS   Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, 
            Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, 
            Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
  TITLE     A cleavage product of Polycystin-1 is a mitochondrial matrix protein
            that affects mitochondria morphology and function when 
            heterologously expressed.
  JOURNAL   Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6.
  PUBMED    29426897
REFERENCE   2  (bases 1 to 19894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-02-9"; volume: "8(1):2743. doi"; pages: " 
            10.1038/s41598-018-20856-6"
COMMENT     Sequence Label: pcDNA5-eGFP-PC1-3HA
FEATURES             Location/Qualifiers
     source          1..19894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2576..2594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2625..2649
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2740..2872
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2855..2874
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2917..2935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2966..3538
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3546..3565
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(3884..3886,3887..3889,3890..4600)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3884..3886,3887..3889,3890..4600)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3929..3950)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4190..4209)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4537..4558
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             17444..17524
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(17602..17619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17608..17832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(17965..17984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    18116..18163
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             18171..19193
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             18171..19193
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    19323..19444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(19360..19379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     19414..19433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(19493..19509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19493..19509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(19506..19528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19517..19533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19541..19547,19548..19565,19566..19571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19586..19607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(19724..19741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct
     2581 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
     2641 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     2701 cgatccagcc tccggactct agcgtttaaa cttaagcttg taagtatcaa ggttacaaga
     2761 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     2821 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
     2881 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagt
     2941 ctggatcgat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
     3001 cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat
     3061 gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct
     3121 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
     3181 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
     3241 taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc
     3301 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
     3361 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
     3421 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
     3481 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     3541 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc
     3601 gtcgacagcg tccgagcggg cggccgagct cccggagcgg cctggccccg agccccgagc
     3661 gggcgtcgct cagcagcagg tcgcggccgc agccccatcc agccccgcgc ccgccatgcc
     3721 gtccgcgggc cccgcctgag ctgcggcctc cgcgcgcggg cgggcctggg gacggcgggg
     3781 ccatgcgcgc gctgccctaa cgatgccgcc cgccgcgccc gcccgcctgg cgctggccct
     3841 gggcctgggc ctgtggctcg gggcgctggc ggggggcccc ggcatggtga gcaagggcga
     3901 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3961 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4021 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4081 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4141 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4201 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4261 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4321 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4381 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4441 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4501 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4561 cgccgccggg atcactctcg gcatggacga gctgtacaag tactcagatc ccgggcgcgg
     4621 ctgcgggccc tgcgagcccc cctgcctctg cggcccagcg cccggcgccg cctgccgcgt
     4681 caactgctcg ggccgcgggc tgcggacgct cggtcccgcg ctgcgcatcc ccgcggacgc
     4741 cacagcgcta gacgtctccc acaacctgct ccgggcgctg gacgttgggc tcctggcgaa
     4801 cctctcggcg ctggcagagc tggatataag caacaacaag atttctacgt tagaagaagg
     4861 aatatttgct aatttattta atttaagtga aataaacctg agtgggaacc cgtttgagtg
     4921 tgactgtggc ctggcgtggc tgccgcgatg ggcggaggag cagcaggtgc gggtggtgca
     4981 gcccgaggca gccacgtgtg ctgggcctgg ctccctggct ggccagcctc tgcttggcat
     5041 ccccttgctg gacagtggct gtggtgagga gtatgtcgcc tgcctccctg acaacagctc
     5101 aggcaccgtg gcagcagtgt ccttttcagc tgcccacgaa ggcctgcttc agccagaggc
     5161 ctgcagcgcc ttctgcttct ccaccggcca gggcctcgca gccctctcgg agcagggctg
     5221 gtgcctgtgt ggggcggccc agccctccag tgcctccttt gcctgcctgt ccctctgctc
     5281 cggccccccg ccacctcctg cccccacctg taggggcccc accctcctcc agcacgtctt
     5341 ccctgcctcc ccaggggcca ccctggtggg gccccacgga cctctggcct ctggccagct
     5401 agcagccttc cacatcgctg ccccgctccc tgtcactgcc acacgctggg acttcggaga
     5461 cggctccgcc gaggtggatg ccgctgggcc ggctgcctcg catcgctatg tgctgcctgg
     5521 gcgctatcac gtgacggccg tgctggccct gggggccggc tcagccctgc tggggacaga
     5581 cgtgcaggtg gaagcggcac ctgccgccct ggagctcgtg tgcccgtcct cggtgcagag
     5641 tgacgagagc cttgacctca gcatccagaa ccgcggtggt tcaggcctgg aggccgccta
     5701 cagcatcgtg gccctgggcg aggagccggc ccgagcggtg cacccgctct gcccctcgga
     5761 cacggagatc ttccctggca acgggcactg ctaccgcctg gtggtggaga aggcggcctg
     5821 gctgcaggcg caggagcagt gtcaggcctg ggccggggcc gccctggcaa tggtggacag
     5881 tcccgccgtg cagcgcttcc tggtctctcg ggtcaccagg agcctagacg tgtggatcgg
     5941 cttctcgact gtgcaggggg tggaggtggg cccagcgccg cagggcgagg ccttcagcct
     6001 ggagagctgc cagaactggc tgcccgggga gccacaccca gccacagccg agcactgcgt
     6061 ccggctcggg cccaccgggt ggtgtaacac cgacctgtgc tcagcgccgc acagctacgt
     6121 ctgcgagctg cagcccggag gcccagtgca ggatgccgag aacctcctcg tgggagcgcc
     6181 cagtggggac ctgcagggac ccctgacgcc tctggcacag caggacggcc tctcagcccc
     6241 gcacgagccc gtggaggtca tggtattccc gggcctgcgt ctgagccgtg aagccttcct
     6301 caccacggcc gaatttggga cccaggagct ccggcggccc gcccagctgc ggctgcaggt
     6361 gtaccggctc ctcagcacag cagggacccc ggagaacggc agcgagcctg agagcaggtc
     6421 cccggacaac aggacccagc tggcccccgc gtgcatgcca gggggacgct ggtgccctgg
     6481 agccaacatc tgcttgccgc tggacgcctc ctgccacccc caggcctgcg ccaatggctg
     6541 cacgtcaggg ccagggctac ccggggcccc ctatgcgcta tggagagagt tcctcttctc
     6601 cgttcccgcg gggccccccg cgcagtactc ggtcaccctc cacggccagg atgtcctcat
     6661 gctccctggt gacctcgttg gcttgcagca cgacgctggc cctggcgccc tcctgcactg
     6721 ctcgccggct cccggccacc ctggtcccca ggccccgtac ctctccgcca acgcctcgtc
     6781 atggctgccc cacttgccag cccagctgga gggcacttgg gcctgccctg cctgtgccct
     6841 gcggctgctt gcagccacgg aacagctcac cgtgctgctg ggcttgaggc ccaaccctgg
     6901 actgcggctg cctgggcgct atgaggtccg ggcagaggtg ggcaatggcg tgtccaggca
     6961 caacctctcc tgcagctttg acgtggtctc cccagtggct gggctgcggg tcatctaccc
     7021 tgccccccgc gacggccgcc tctacgtgcc caccaacggc tcagccttgg tgctccaggt
     7081 ggactctggt gccaacgcca cggccacggc tcgctggcct gggggcagtg tcagcgcccg
     7141 ctttgagaat gtctgccctg ccctggtggc caccttcgtg cccggctgcc cctgggagac
     7201 caacgatacc ctgttctcag tggtagcact gccgtggctc agtgaggggg agcacgtggt
     7261 ggacgtggtg gtggaaaaca gcgccagccg ggccaacctc agcctgcggg tgacggcgga
     7321 ggagcccatc tgtggcctcc gcgccacgcc cagccccgag gcccgtgtac tgcagggagt
     7381 cctagtgagg tacagccccg tggtggaggc cggctcggac atggtcttcc ggtggaccat
     7441 caacgacaag cagtccctga ccttccagaa cgtggtcttc aatgtcattt atcagagcgc
     7501 ggcggtcttc aagctctcac tgacggcccc caaccacgtg agcaacgtca ccgtgaacta
     7561 caacgtaacc gtggagcgga tgaacaggat gcagggtctg caggtctcca cagtgccggc
     7621 cgtgctgtcc cccaatgcca cgctggcact gacggcgggc gtgctggtgg actcggccgt
     7681 ggaggtggcc ttcctgtgga cctttgggga tggggagcag gccctccacc agttccagcc
     7741 tccgtacaac gagtccttcc cggttccaga cccctcggtg gcccaggtgc tggtggagca
     7801 caatgtcatg cacacctacg ctgccccagg tgagtacctc ctgaccgtgc tggcatctaa
     7861 tgccttcgag aacctgacgc agcaggtgcc tgtgagcgtg cgcgcctccc tgccctccgt
     7921 ggctgtgggt gtgagtgacg gcgtcctggt ggccggccgg cccgtcacct tctacccgca
     7981 cccgctgccc tcgcctgggg gtgttcttta cacgtgggac ttcggggacg gctcccctgt
     8041 cctgacccag agccagccgg ctgccaacca cacctatgcc tcgaggggca cctaccacgt
     8101 gcgcctggag gtcaacaaca cggtgagcgg tgcggcggcc caggcggatg tgcgcgtctt
     8161 tgaggagctc cgcggactca gcgtggacat gagcctggcc gtggagcagg gcgcccccgt
     8221 ggtggtcagc gccgcggtgc agacgggcga caacatcacg tggaccttcg acatggggga
     8281 cggcaccgtg ctgtcgggcc cggaggcaac agtggagcat gtgtacctgc gggcacagaa
     8341 ctgcacagtg accgtgggtg cggccagccc cgccggccac ctggcccgga gcctgcacgt
     8401 gctggtcttc gtcctggagg tgctgcgcgt tgaacccgcc gcctgcatcc ccacgcagcc
     8461 tgacgcgcgg ctcacggcct acgtcaccgg gaacccggcc cactacctct tcgactggac
     8521 cttcggggat ggctcctcca acacgaccgt gcgggggtgc ccgacggtga cacacaactt
     8581 cacgcggagc ggcacgttcc ccctggcgct ggtgctgtcc agccgcgtga acagggcgca
     8641 ttacttcacc agcatctgcg tggagccaga ggtgggcaac gtcaccctgc agccagagag
     8701 gcagtttgtg cagctcgggg acgaggcctg gctggtggca tgtgcctggc ccccgttccc
     8761 ctaccgctac acctgggact ttggcaccga ggaagccgcc cccacccgtg ccaggggccc
     8821 tgaggtgacg ttcatctacc gagacccagg ctcctatctt gtgacagtca ccgcgtccaa
     8881 caacatctct gctgccaatg actcagccct ggtggaggtg caggagcccg tgctggtcac
     8941 cagcatcaag gtcaatggct cccttgggct ggagctgcag cagccgtacc tgttctctgc
     9001 tgtgggccgt gggcgccccg ccagctacct gtgggatctg ggggacggtg ggtggctcga
     9061 gggtccggag gtcacccacg cttacaacag cacaggtgac ttcaccgtta gggtggccgg
     9121 ctggaatgag gtgagccgca gcgaggcctg gctcaatgtg acggtgaagc ggcgcgtgcg
     9181 ggggctcgtc gtcaatgcaa gccgcacggt ggtgcccctg aatgggagcg tgagcttcag
     9241 cacgtcgctg gaggccggca gtgatgtgcg ctattcctgg gtgctctgtg accgctgcac
     9301 gcccatccct gggggtccta ccatctctta caccttccgc tccgtgggca ccttcaatat
     9361 catcgtcacg gctgagaacg aggtgggctc cgcccaggac agcatcttcg tctatgtcct
     9421 gcagctcata gaggggctgc aggtggtggg cggtggccgc tacttcccca ccaaccacac
     9481 ggtacagctg caggccgtgg ttagggatgg caccaacgtc tcctacagct ggactgcctg
     9541 gagggacagg ggcccggccc tggccggcag cggcaaaggc ttctcgctca ccgtgctcga
     9601 ggccggcacc taccatgtgc agctgcgggc caccaacatg ctgggcagcg cctgggccga
     9661 ctgcaccatg gacttcgtgg agcctgtggg gtggctgatg gtggccgcct ccccgaaccc
     9721 agctgccgtc aacacaagcg tcaccctcag tgccgagctg gctggtggca gtggtgtcgt
     9781 atacacttgg tccttggagg aggggctgag ctgggagacc tccgagccat ttaccaccca
     9841 tagcttcccc acacccggcc tgcacttggt caccatgacg gcagggaacc cgctgggctc
     9901 agccaacgcc accgtggaag tggatgtgca ggtgcctgtg agtggcctca gcatcagggc
     9961 cagcgagccc ggaggcagct tcgtggcggc cgggtcctct gtgccctttt gggggcagct
    10021 ggccacgggc accaatgtga gctggtgctg ggctgtgccc ggcggcagca gcaagcgtgg
    10081 ccctcatgtc accatggtct tcccggatgc tggcaccttc tccatccggc tcaatgcctc
    10141 caacgcagtc agctgggtct cagccacgta caacctcacg gcggaggagc ccatcgtggg
    10201 cctggtgctg tgggccagca gcaaggtggt ggcgcccggg cagctggtcc attttcagat
    10261 cctgctggct gccggctcag ctgtcacctt ccgcctgcag gtcggcgggg ccaaccccga
    10321 ggtgctcccc gggccccgtt tctcccacag cttcccccgc gtcggagacc acgtggtgag
    10381 cgtgcggggc aaaaaccacg tgagctgggc ccaggcgcag gtgcgcatcg tggtgctgga
    10441 ggccgtgagt gggctgcagg tgcccaactg ctgcgagcct ggcatcgcca cgggcactga
    10501 gaggaacttc acagcccgcg tgcagcgcgg ctctcgggtc gcctacgcct ggtacttctc
    10561 gctgcagaag gtccagggcg actcgctggt catcctgtcg ggccgcgacg tcacctacac
    10621 gcccgtggcc gcggggctgt tggagatcca ggtgcgcgcc ttcaacgccc tgggcagtga
    10681 gaaccgcacg ctggtgctgg aggttcagga cgccgtccag tatgtggccc tgcagagcgg
    10741 cccctgcttc accaaccgct cggcgcagtt tgaggccgcc accagcccca gcccccggcg
    10801 tgtggcctac cactgggact ttggggatgg gtcgccaggg caggacacag atgagcccag
    10861 ggccgagcac tcctacctga ggcctgggga ctaccgcgtg caggtgaacg cctccaacct
    10921 ggtgagcttc ttcgtggcgc aggccacggt gaccgtccag gtgctggcct gccgggagcc
    10981 ggaggtggac gtggtcctgc ccctgcaggt gctgatgcgg cgatcacagc gcaactactt
    11041 ggaggcccac gttgacctgc gcgactgcgt cacctaccag actgagtacc gctgggaggt
    11101 gtatcgcacc gccagctgcc agcggccggg gcgcccagcg cgtgtggccc tgcccggcgt
    11161 ggacgtgagc cggcctcggc tggtgctgcc gcggctggcg ctgcctgtgg ggcactactg
    11221 ctttgtgttt gtcgtgtcat ttggggacac gccactgaca cagagcatcc aggccaatgt
    11281 gacggtggcc cccgagcgcc tggtgcccat cattgagggt ggctcatacc gcgtgtggtc
    11341 agacacacgg gacctggtgc tggatgggag cgagtcctac gaccccaacc tggaggacgg
    11401 cgaccagacg ccgctcagtt tccactgggc ctgtgtggct tcgacacaga gggaggctgg
    11461 cgggtgtgcg ctgaactttg ggccccgcgg gagcagcacg gtcaccattc cacgggagcg
    11521 gctggcggct ggcgtggagt acaccttcag cctgaccgtg tggaaggccg gccgcaagga
    11581 ggaggccacc aaccagacgg tgctgatccg gagtggccgg gtgcccattg tgtccttgga
    11641 gtgtgtgtcc tgcaaggcac aggccgtgta cgaagtgagc cgcagctcct acgtgtactt
    11701 ggagggccgc tgcctcaatt gcagcagcgg ctccaagcga gggcggtggg ctgcacgtac
    11761 gttcagcaac aagacgctgg tgctggatga gaccaccaca tccacgggca gtgcaggcat
    11821 gcgactggtg ctgcggcggg gcgtgctgcg ggacggcgag ggatacacct tcacgctcac
    11881 ggtgctgggc cgctctggcg aggaggaggg ctgcgcctcc atccgcctgt cccccaaccg
    11941 cccgccgctg gggggctctt gccgcctctt cccactgggc gctgtgcacg ccctcaccac
    12001 caaggtgcac ttcgaatgca cgggctggca tgacgcggag gatgctggcg ccccgctggt
    12061 gtacgccctg ctgctgcggc gctgtcgcca gggccactgc gaggagttct gtgtctacaa
    12121 gggcagcctc tccagctacg gagccgtgct gcccccgggt ttcaggccac acttcgaggt
    12181 gggcctggcc gtggtggtgc aggaccagct gggagccgct gtggtcgccc tcaacaggtc
    12241 tttggccatc accctcccag agcccaacgg cagcgcaacg gggctcacag tctggctgca
    12301 cgggctcacc gctagtgtgc tcccagggct gctgcggcag gccgatcccc agcacgtcat
    12361 cgagtactcg ttggccctgg tcaccgtgct gaacgagtac gagcgggccc tggacgtggc
    12421 ggcagagccc aagcacgagc ggcagcaccg agcccagata cgcaagaaca tcacggagac
    12481 tctggtgtcc ctgagggtcc acactgtgga tgacatccag cagatcgctg ctgcgctggc
    12541 ccagtgcatg gggcccagca gggagctcgt atgccgctcg tgcctgaagc agacgctgca
    12601 caagctggag gccatgatgc tcatcctgca ggcagagacc accgcgggca ccgtgacgcc
    12661 caccgccatc ggagacagca tcctcaacat cacaggagac ctcatccacc tggccagctc
    12721 ggacgtgcgg gcaccacagc cctcagagct gggagccgag tcaccatctc ggatggtggc
    12781 gtcccaggcc tacaacctga cctctgccct catgcgcatc ctcatgcgct cccgcgtgct
    12841 caacgaggag cccctgacgc tggcgggcga ggagatcgtg gcccagggca agcgctcgga
    12901 cccgcggagc ctgctgtgct atggcggcgc cccagggcct ggctgccact tctccatccc
    12961 cgaggctttc agcggggccc tggccaacct cagtgacgtg gtgcagctca tctttctggt
    13021 ggactccaat ccctttccct ttggctatat cagcaactac accgtctcca ccaaggtggc
    13081 ctcgatggca ttccagacac aggccggcgc ccagatcccc atcgagcggc tggcctcaga
    13141 gcgcgccatc accgtgaagg tgcccaacaa ctcggactgg gctgcccggg gccaccgcag
    13201 ctccgccaac tccgccaact ccgttgtggt ccagccccag gcctccgtcg gtgctgtggt
    13261 caccctggac agcagcaacc ctgcggccgg gctgcatctg cagctcaact atacgctgct
    13321 ggacggccac tacctgtctg aggaacctga gccctacctg gcagtctacc tacactcgga
    13381 gccccggccc aatgagcaca actgctcggc tagcaggagg atccgcccag agtcactcca
    13441 gggtgctgac caccggccct acaccttctt catttccccg gggagcagag acccagcggg
    13501 gagttaccat ctgaacctct ccagccactt ccgctggtcg gcgctgcagg tgtccgtggg
    13561 cctgtacacg tccctgtgcc agtacttcag cgaggaggac atggtgtggc ggacagaggg
    13621 gctgctgccc ctggaggaga cctcgccccg ccaggccgtc tgcctcaccc gccacctcac
    13681 cgccttcggc gccagcctct tcgtgccccc aagccatgtc cgctttgtgt ttcctgagcc
    13741 gacagcggat gtaaactaca tcgtcatgct gacatgtgct gtgtgcctgg tgacctacat
    13801 ggtcatggcc gccatcctgc acaagctgga ccagttggat gccagccggg gccgcgccat
    13861 ccctttctgt gggcagcggg gccgcttcaa gtacgagatc ctcgtcaaga caggctgggg
    13921 ccggggctca ggtaccacgg cccacgtggg catcatgctg tatggggtgg acagccggag
    13981 cggccaccgg cacctggacg gcgacagagc cttccaccgc aacagcctgg acatcttccg
    14041 gatcgccacc ccgcacagcc tgggtagcgt gtggaagatc cgagtgtggc acgacaacaa
    14101 agggctcagc cctgcctggt tcctgcagca cgtcatcgtc agggacctgc agacggcacg
    14161 cagcgccttc ttcctggtca atgactggct ttcggtggag acggaggcca acgggggcct
    14221 ggtggagaag gaggtgctgg ccgcgagcga cgcagccctt ttgcgcttcc ggcgcctgct
    14281 ggtggctgag ctgcagcgtg gcttctttga caagcacatc tggctctcca tatgggaccg
    14341 gccgcctcgt agccgtttca ctcgcatcca gagggccacc tgctgcgttc tcctcatctg
    14401 cctcttcctg ggcgccaacg ccgtgtggta cggggctgtt ggcgactctg cctacagcac
    14461 ggggcatgtg tccaggctga gcccgctgag cgtcgacaca gtcgctgttg gcctggtgtc
    14521 cagcgtggtt gtctatcccg tctacctggc catccttttt ctcttccgga tgtcccggag
    14581 caaggtggct gggagcccga gccccacacc tgccgggcag caggtgctgg acatcgacag
    14641 ctgcctggac tcgtccgtgc tggacagctc cttcctcacg ttctcaggcc tccacgctga
    14701 ggcctttgtt ggacagatga agagtgactt gtttctggat gattctaaga gtctggtgtg
    14761 ctggccctcc ggcgagggaa cgctcagttg gccggacctg ctcagtgacc cgtccattgt
    14821 gggtagcaat ctgcggcagc tggcacgggg ccaggcgggc catgggctgg gcccagagga
    14881 ggacggcttc tccctggcca gcccctactc gcctgccaaa tccttctcag catcagatga
    14941 agacctgatc cagcaggtcc ttgccgaggg ggtcagcagc ccagccccta cccaagacac
    15001 ccacatggaa acggacctgc tcagcagcct gtccagcact cctggggaga agacagagac
    15061 gctggcgctg cagaggctgg gggagctggg gccacccagc ccaggcctga actgggaaca
    15121 gccccaggca gcgaggctgt ccaggacagg actggtggag ggtctgcgga agcgcctgct
    15181 gccggcctgg tgtgcctccc tggcccacgg gctcagcctg ctcctggtgg ctgtggctgt
    15241 ggctgtctca gggtgggtgg gtgcgagctt ccccccgggc gtgagtgttg cgtggctcct
    15301 gtccagcagc gccagcttcc tggcctcatt cctcggctgg gagccactga aggtcttgct
    15361 ggaagccctg tacttctcac tggtggccaa gcggctgcac ccggatgaag atgacaccct
    15421 ggtagagagc ccggctgtga cgcctgtgag cgcacgtgtg ccccgcgtac ggccacccca
    15481 cggctttgca ctcttcctgg ccaaggaaga agcccgcaag gtcaagaggc tacatggcat
    15541 gctgcggagc ctcctggtgt acatgctttt tctgctggtg accctgctgg ccagctatgg
    15601 ggatgcctca tgccatgggc acgcctaccg tctgcaaagc gccatcaagc aggagctgca
    15661 cagccgggcc ttcctggcca tcacgcggtc tgaggagctc tggccatgga tggcccacgt
    15721 gctgctgccc tacgtccacg ggaaccagtc cagcccagag ctggggcccc cacggctgcg
    15781 gcaggtgcgg ctgcaggaag cactctaccc agaccctccc ggccccaggg tccacacgtg
    15841 ctcggccgca ggaggcttca gcaccagcga ttacgacgtt ggctgggaga gtcctcacaa
    15901 tggctcgggg acgtgggcct attcagcgcc ggatctgctg ggggcatggt cctggggctc
    15961 ctgtgccgtg tatgacagcg ggggctacgt gcaggagctg ggcctgagcc tggaggagag
    16021 ccgcgaccgg ctgcgcttcc tgcagctgca caactggctg gacaacagga gccgcgctgt
    16081 gttcctggag ctcacgcgct acagcccggc cgtggggctg cacgccgccg tcacgctgcg
    16141 cctcgagttc ccggcggccg gccgcgccct ggccgccctc agcgtccgcc cctttgcgct
    16201 gcgccgcctc agcgcgggcc tctcgctgcc tctgctcacc tcggtgtgcc tgctgctgtt
    16261 cgccgtgcac ttcgccgtgg ccgaggcccg tacttggcac agggaagggc gctggcgcgt
    16321 gctgcggctc ggagcctggg cgcggtggct gctggtggcg ctgacggcgg ccacggcact
    16381 ggtacgcctc gcccagctgg gtgccgctga ccgccagtgg acccgtttcg tgcgcggccg
    16441 cccgcgccgc ttcactagct tcgaccaggt ggcgcagctg agctccgcag cccgtggcct
    16501 ggcggcctcg ctgctcttcc tgcttttggt caaggctgcc cagcagctac gcttcgtgcg
    16561 ccagtggtcc gtctttggca agacattatg ccgagctctg ccagagctcc tgggggtcac
    16621 cttgggcctg gtggtgctcg gggtagccta cgcccagctg gccatcctgc tcgtgtcttc
    16681 ctgtgtggac tccctctgga gcgtggccca ggccctgttg gtgctgtgcc ctgggactgg
    16741 gctctctacc ctgtgtcctg ccgagtcctg gcacctgtca cccctgctgt gtgtggggct
    16801 ctgggcactg cggctgtggg gcgccctacg gctgggggct gttattctcc gctggcgcta
    16861 ccacgccttg cgtggagagc tgtaccggcc ggcctgggag ccccaggact acgagatggt
    16921 ggagttgttc ctgcgcaggc tgcgcctctg gatgggcctc agcaaggtca aggagttccg
    16981 ccacaaagtc cgctttgaag ggatggagcc gctgccctct cgctcctcca ggggctccaa
    17041 ggtatccccg gatgtgcccc cacccagcgc tggctccgat gcctcgcacc cctccacctc
    17101 ctccagccag ctggatgggc tgagcgtgag cctgggccgg ctggggacaa ggtgtgagcc
    17161 tgagccctcc cgcctccaag ccgtgttcga ggccctgctc acccagtttg accgactcaa
    17221 ccaggccaca gaggacgtct accagctgga gcagcagctg cacagcctgc aaggccgcag
    17281 gagcagccgg gcgcccgccg gatcttcccg tggcccatcc ccgggcctgc ggccagcact
    17341 gcccagccgc cttgcccggg ccagtcgggg tgtggacctg gccactggcc ccagcaggac
    17401 accccttcgg gccaagaaca aggtccaccc cagcagcacc ggttatccat atgatgttcc
    17461 agattatgct tatccatatg atgttccaga ttatgcttat ccatatgatg ttccagatta
    17521 tgcttgatca ggtagcttat cgatatccag cacagtggcg gccgctcgag tctagagggc
    17581 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    17641 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    17701 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    17761 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    17821 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
    17881 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    17941 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    18001 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    18061 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt
    18121 tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg
    18181 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
    18241 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
    18301 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
    18361 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
    18421 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
    18481 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
    18541 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
    18601 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
    18661 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
    18721 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
    18781 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
    18841 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
    18901 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
    18961 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
    19021 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    19081 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    19141 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac
    19201 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    19261 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    19321 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    19381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    19441 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    19501 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    19561 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    19621 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    19681 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    19741 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    19801 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    19861 ccaggaaccg taaaaaggcc gcgttgctgg cgtt
//
