LOCUS       sequence-218683-       10726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression of a constitutively active form of the Glis3 
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPG-UBI-Glis3M412
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1219..1243
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2883..2907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2924..3434
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3353..3371
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3450..3459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3456..3458,3459..3461,3462..4175)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3456..3458,3459..3461,3462..4172)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3501..3522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3762..3781)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4109..4130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4191..4779
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4244..4264)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4662..4673)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4920..4953)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4973..5153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5191..5210)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5256..5311
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5310..5329)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5672..5688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5672..5688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5685..5707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5696..5712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5720..5726,5727..5744,5745..5750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5765..5786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5845..6040
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5891..6026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6046..6180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6137..6156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6248..6265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6419..7006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6499..6518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7177..7968,7969..8037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7800..7819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8038..8142)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8173..8552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8554..8811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8858..8983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9475..9592
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9952..10185
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10370..10414
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10689..10709
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 agccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acaggatccc aactttgtac aaaaaagcag gctactagtg aggaaccaat
     1261 tcagtcgact ggatccggta ccgaattgat ccaccatggt gttgcagcct ggcccggtag
     1321 gccaggatgg ccagacagcc aacatgctca aaacagagcg cctggaggag ttccccgcca
     1381 gtgccttgga cctgccctct gctctgcctc tccctcttcc tccgcctcag ggtcccccac
     1441 ccccatacca tgcccatcca caccttcatc acccagagct cctgcctcac acccaatcgc
     1501 tgtccctggc ccagactggc ctggaagagg atggggagat ggaagactca ggggggaagc
     1561 actgctgccg ttggatagac tgcagtgccc tttatgacca gcaggaggaa ctggtgaggc
     1621 acatcgagaa ggtccacata gaccaacgca agggggaaga cttcacgtgc ttctggactg
     1681 gctgccctag aagatacaag cctttcaacg cacggtatag actgctgatc cacatgaggg
     1741 tccactctgg ggagaagccc aacaagtgta cgttcgaagg ctgcaagaag gccttttcca
     1801 ggctcgagaa cctcaagatt cacttgcgga gccacacggg tgagaagcca tacttgtgcc
     1861 agcatccggg ctgccaaaag gccttcagca attccagtga ccgtgccaag caccagagga
     1921 cacatcttga cactaaacct tatgcttgtc aaattccagg atgtacaaag cgctacacag
     1981 acccaagctc cctcagaaag cacgtgaagg cacattcttc ccgagagcag caagcaagga
     2041 aaaagctacg gtctagcact gagctccatc cagatctcct cacagactgc ctcgctgtgc
     2101 agcccctgca gccagccacg tcccctggag atgctgctga tcataccgtg gggcactccc
     2161 cagggccggg gcctgggcca gggcctgggg ctgaactcta ttcagctccc attttcgcca
     2221 gcaatcattc aactcgaagt ggagcagctg ctggggctgg gccaccccca catcctgtca
     2281 gtcgcccttc tccaggacat aatgtacagg ggagccccca caacccctcc tcccagttac
     2341 ctccactcac agctgtggac gcaggcgctg agaggtttgc acctccgact ccatctcctc
     2401 accacatcag cccgggaaga gttccagctc ctccgtcact gctccagaga gcacaggctc
     2461 cccactccca gcagccacca ggctcactcc tgaagcccta ccagcctgaa accaactctt
     2521 cttttcagcc aaatggtatc catgtccacg gattttatgg ccagctgcag acattctgtc
     2581 ccccgcatta tcctgattcc caaagaactg tgccacccag cggctcctgc agtatggtgc
     2641 cttcctttga ggactgcctg gtccccacgt ccatgggtca agccggtttt gatgttttcc
     2701 acagagcctt ctcaacacac tcaggcatca cagtgtacga tttgccttca gcttcctcaa
     2761 gcctctttgg ggaatctctt cgcagtggcc ctgaagaccc cacgttcttg cagctcagtg
     2821 ctgtggaccg ctgtcctagc cagctctcct ctgtgtatac cgaaggctga gcggccatct
     2881 agacccagct ttcttgtaca aagtggtgat aatcgaattc cacggggttg gggttgcgcc
     2941 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     3001 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     3061 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     3121 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     3181 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     3241 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     3301 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     3361 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     3421 acctctctcc ccagggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     3481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3541 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3661 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3721 tgcccgaagg ctatgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3781 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3841 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3901 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3961 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4021 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4081 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4141 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc
     4201 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4261 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4321 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4381 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4441 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4501 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4561 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4621 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4681 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4741 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattccc gcggttcgct
     4801 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4861 ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc accatgggaa
     4921 taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct
     4981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5161 gttgaatcgc cagtgtgtcg agctagcaga tctttttccc tctgccaaaa attatgggga
     5221 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     5281 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     5341 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     5401 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     5461 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     5521 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     5581 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     5641 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5701 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5761 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5821 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     5881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6001 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     6061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     6181 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6541 ctcctgttcc gaccctgccg cttacggata cctgtccgcc tttctccctt cgggaagcgt
     6601 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6661 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6721 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6781 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6841 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6901 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6961 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7021 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7081 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7141 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7201 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7261 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7321 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7381 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7441 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7501 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7561 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7621 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7681 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7741 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7801 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7861 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7921 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7981 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8161 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     8221 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     8281 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     8341 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     8401 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     8461 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8521 acatctacgt attagtcatc gctattacca tgggaggcgt ggcctgggcg ggactgggga
     8581 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     8641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8821 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8881 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8941 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     9001 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     9061 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaata ctggcctgtt
     9121 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     9181 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     9241 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9301 taagaaaaaa gcacagcaag cagcaggatc ttcagacctg gaaattccct acaatcccca
     9361 aagtcaagga gtagtagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
     9421 agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc acaattttaa
     9481 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9541 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     9601 ttacagggac agcagaaatc cactttggaa aggaccagca aagctcctct ggaaaggtga
     9661 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
     9721 gatcattagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
     9781 tgaggattag aacatggaaa agtttagtaa aacaccataa ggaggagata tgagggacaa
     9841 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9901 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9961 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10021 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    10081 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10141 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10201 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10261 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    10321 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10381 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10441 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10501 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10561 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    10621 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atctagtgat
    10681 tatcggatca actttgtata gaaaagttgt taattaaggg tgcagc
//
