LOCUS       sequence-218686-        5937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP12-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..5937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             667..1626
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             complement(2458..2469)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     polyA_signal    2779..2900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2816..2835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2870..2889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2907..3362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3044..3065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3256..3275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3389..3493
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3495..3852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3495..3686
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3518..3538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3703..3838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3887..4681
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3887..4681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3941..3960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4551..4570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4869..4888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4913..4960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5289..5877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5778..5797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatgt cggtaccgct gactacctca gcattcggcc acgcgtttct ggctaactgt
      721 gaacgcgagc agatccacct ggcgggctcc attcagccgc acggtatcct gctggctgtg
      781 aaagagccgg acaacgtggt gatccaggct tctattaacg ctgcggagtt cctgaacacc
      841 aactctgttg ttggccgtcc gctgcgtgac ctgggcggcg atctgccttt gcagatcctg
      901 ccgcacctga acggcccgct gcacctggct ccgatgaccc tgcgttgtac cgtgggttct
      961 ccgccgcgtc gtgtggactg taccattcat cgtccgtcta acggcggcct gatcgtagaa
     1021 ctggaaccag caaccaagac cactaacatt gcgccggctc tggacggtgc gtttcatcgt
     1081 atcacttctt catcctccct gatgggcctg tgtgacgaaa ccgcgactat tatccgtgag
     1141 attactggct acgaccgtgt gatggtagta cgtttcgatg aagagggtaa tggcgaaatt
     1201 ctgtccgaac gtcgtcgtgc ggacctggaa gcgttcctgg gtaaccgcta cccggcgtct
     1261 actattccgc agatcgctcg tcgcctgtac gaacataacc gtgttcgcct gctggtagat
     1321 gtgaactata ctccggttcc gctacagccg cgcatcagcc cgctgaacgg tcgtgatctg
     1381 gatatgtccc tgtcttgcct gcgctctatg tccccgatcc accagaaata catgcaggac
     1441 atgggcgttg gcgcgaccct ggtttgctct ctgatggtgt ctggtcgtct gtggggtctg
     1501 atcgcttgcc accactacga accgcgcttc gttccgttcc acattcgcgc tgctggcgaa
     1561 gcgctggcgg aaacttgtgc gatccgcatc gcgacgctgg agagctttgc acagtctcag
     1621 tccaaaggtg gcggaagcgg cggcggaagc ggtggcggaa gttccggact cagatctcga
     1681 gccatgaagc caggattcag tccccgtggg ggtggctttg gcggccgagg gggctttggt
     1741 gaccgtggtg gtcgtggagg ccgagggggc tttggcgggg gccgaggcca aggcggaggc
     1801 tttagaggtc gtggacgagg aggaggtgga ggcggcggcg gcggtggagg aggaggaaga
     1861 ggtggtggag gcttccattt tggtggcaac cggggtcgtg gtcggggagg aaaaagagga
     1921 aaccagtcgg ggaagaatgt gatggtggag ccgcatcggc atgagggtgt cttcatttgt
     1981 cggggaaagg aagatgcact ggtcaccaag aacctggtcc ctggggaatc agtttatgga
     2041 gagaagagag tctcgatttc ggaaggagat gacaaaattg agtaccgagc ctggaacccc
     2101 ttccgctcca agcttgcagc agcaatcctg ggtggtgtgg accagatcca catcaaaccg
     2161 ggggctaagg ttctctacct cggggctgcc tcgggcacca cggtctccca tgtctctgac
     2221 atcgttggtc cggatggtct agtctatgca gtcgagttct cccaccgctc tggccgtgac
     2281 ctcattaact tggccaagaa gaggaccaac atcattcctg tgatcgagga tgctcgacac
     2341 ccacacaaat accgcatgct catcgcaatg gtggatgtga tctttgctga tgtggcccag
     2401 ccagaccaga cccggattgt ggccctgaat gcccacacct tcctgcgtaa tggaggacac
     2461 tttgtgattt ccattaaggc caactgcatt gactccacag cctcagccga ggccgtgttt
     2521 gcctccgaag tgaaaaagat gcaacaggag aacatgaagc cgcaggagca gttgaccctt
     2581 gagccatatg aaagagacca tgccgtggtc gtgggagtgt acaggccacc ccccaaggtg
     2641 aagaactgag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2701 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2761 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2881 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaacttcgc
     2941 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3001 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3061 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3121 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3181 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3241 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3301 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3481 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3841 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4381 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4681 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4741 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4801 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4861 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4921 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4981 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5041 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5101 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5161 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg
//
