LOCUS       sequence-218690-        5988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5988)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T2-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..5988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             667..1398
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTETLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    2902..3023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2939..2958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2993..3012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3030..3485)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3167..3188)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3379..3398
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3512..3616
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3641..3661)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3685..3895
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3707..3903
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3754..3889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3938..4732
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3938..4732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3992..4011)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4602..4621
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4920..4939)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4964..5011
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5340..5928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5829..5848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
      721 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
      781 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      841 ttcgacatcc tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag
      901 ggcatccccg atttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc
      961 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     1021 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag
     1081 aagaaaacac tcggctggga ggccaacacc gagaccctgt accccgctga cggcggcctg
     1141 gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc
     1201 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat
     1261 gtggaccaca gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac
     1321 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaatggc
     1381 atggacgagc tgtacaagtc cggactcaga tctcgagtgc gtgagtgcat ctccatccac
     1441 gttggccagg ctggtgtcca gattggcaat gcctgctggg agctctactg cctggaacac
     1501 ggcatccagc ccgatggcca gatgccaagt gacaagacca ttgggggagg agatgactcc
     1561 ttcaacacct tcttcagtga gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta
     1621 gacttggaac ccacagtcat tgatgaagtt cgcactggca cctaccgcca gctcttccac
     1681 cctgagcagc tcatcacagg caaggaagat gctgccaata actatgcccg agggcactac
     1741 accattggca aggagatcat tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag
     1801 tgcaccggtc ttcagggctt cttggttttc cacagctttg gtgggggaac tggttctggg
     1861 ttcacctccc tgctcatgga acgtctctca gttgattatg gcaagaagtc caagctggag
     1921 ttctccattt acccagcacc ccaggtttcc acagctgtag ttgagcccta caactccatc
     1981 ctcaccaccc acaccaccct ggagcactct gattgtgcct tcatggtaga caatgaggcc
     2041 atctatgaca tctgtcgtag aaacctcgat atcgagcgcc caacctacac taaccttaac
     2101 cgccttatta gccagattgt gtcctccatc actgcttccc tgagatttga tggagccctg
     2161 aatgttgacc tgacagaatt ccagaccaac ctggtgccct acccccgcat ccacttccct
     2221 ctggccacat atgcccctgt catctctgct gagaaagcct accatgaaca gctttctgta
     2281 gcagagatca ccaatgcttg ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc
     2341 catggtaaat acatggcttg ctgcctgttg taccgtggtg acgtggttcc caaagatgtc
     2401 aatgctgcca ttgccaccat caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc
     2461 actggcttca aggttggcat caactaccag cctcccactg tggtgcctgg tggagacctg
     2521 gccaaggtac agagagctgt gtgcatgctg agcaacacca cagccattgc tgaggcctgg
     2581 gctcgcctgg accacaagtt tgacctgatg tatgccaagc gtgcctttgt tcactggtac
     2641 gtgggtgagg ggatggagga aggcgagttt tcagaggccc gtgaagatat ggctgccctt
     2701 gagaaggatt atgaggaggt tggtgtggat tctgttgaag gagagggtga ggaagaagga
     2761 gaggaatact aaggatccac cggatctaga taactgatca taatcagcca taccacattt
     2821 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2881 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     2941 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3001 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3061 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3121 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3181 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3241 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3301 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3361 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3421 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3481 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3541 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3601 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     3661 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3721 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3781 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3841 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3901 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     3961 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4021 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4081 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4141 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4201 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4261 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4321 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4381 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4441 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4501 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4561 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4621 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4681 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4861 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     4921 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     4981 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5041 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5101 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5161 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5461 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatg
//
