LOCUS       sequence-218693-        6551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-C-Src-7
FEATURES             Location/Qualifiers
     source          1..6551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             689..730
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             2309..3268
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    3393..3514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3430..3449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3484..3503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3521..3976)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3658..3679)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3870..3889
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4003..4107
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4109..4466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4109..4300
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4132..4152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4317..4452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4501..5295
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4501..5295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4555..4574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5165..5184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5483..5502)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5527..5574
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5903..6491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6392..6411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattcat ggggagcagc aagagcaagc ccaaggaccc
      721 cagccagcgc cggcgcagcc tggagccacc cgacagcacc caccacgggg gattcccagc
      781 ctcgcagacc cccaacaaga cagcagcccc cgacacgcac cgcaccccca gccgctcctt
      841 tgggaccgtg gccaccgagc ccaagctctt cgggggcttc aacacttctg acaccgtcac
      901 gtcgccgcag cgtgccgggg cactggctgg cggcgtcacc actttcgtgg ctctctatga
      961 ctacgagtcc cggactgaaa cggacttgtc cttcaagaaa ggagaacgcc tgcagattgt
     1021 caacaacacg gaaggtgact ggtggctggc tcattccctc actacaggac agacgggcta
     1081 catccccagt aactatgtcg cgccctcaga ctccatccag gctgaagagt ggtactttgg
     1141 gaagatcact cgtcgggagt ccgagcggct gctgctcaac cccgaaaacc cccggggaac
     1201 cttcttggtc cgggagagcg agacgacaaa aggtgcctat tgcctctccg tttctgactt
     1261 tgacaacgcc aaggggctca atgtgaagca ctacaagatc cgcaagctgg acagcggcgg
     1321 cttctacatc acctcacgca cacagttcag cagcctgcag cagctggtgg cctactactc
     1381 caaacatgct gatggcttgt gccaccgcct gaccaacgtc tgccccacgt ccaagcccca
     1441 gacccaggga ctcgccaagg acgcgtggga aatcccccgg gagtcgctgc ggctggaggt
     1501 gaagctgggg cagggctgct ttggagaggt ctggatgggg acctggaacg gcaccaccag
     1561 agtggccata aagactctga agcccggcac catgtccccg gaggccttcc tgcaggaagc
     1621 ccaagtgatg aagaagctcc ggcatgagaa gctggttcag ctgtacgcag tggtgtcgga
     1681 agagcccatc tacatcgtca ctgagtacat gagcaagggg agcctcctgg atttcctgaa
     1741 gggagagatg ggcaagtacc tgcggctgcc acagctcgtc gatatggctg ctcagattgc
     1801 atccggcatg gcctatgtgg agaggatgaa ctacgtgcac cgagacctgc gggcggccaa
     1861 catcctggtg ggggagaacc tggtgtgcaa ggtggctgac tttgggctgg cacgcctcat
     1921 cgaggacaac gagtacacag cacggcaagg tgccaagttc cccatcaagt ggacagcccc
     1981 cgaggcagcc ctctatggcc ggttcaccat caagtcggat gtctggtcct tcggcatcct
     2041 gctgactgag ctgaccacca agggccgggt gccataccca gggatggtca acagggaggt
     2101 gctggaccag gtggagaggg gctaccgcat gccctgcccg cccgagtgcc ccgagtcgct
     2161 gcatgacctc atgtgccagt gctggcggaa ggaccctgag gagcggccca cttttgagta
     2221 cctgcaggcc ttcctggagg actacttcac ctcgacagag ccccagtacc agcctggaga
     2281 gaacctacgg gatccaccgg tcgccaccat gtcggtaccg ctgactacct cagcattcgg
     2341 ccacgcgttt ctggctaact gtgaacgcga gcagatccac ctggcgggct ccattcagcc
     2401 gcacggtatc ctgctggctg tgaaagagcc ggacaacgtg gtgatccagg cttctattaa
     2461 cgctgcggag ttcctgaaca ccaactctgt tgttggccgt ccgctgcgtg acctgggcgg
     2521 cgatctgcct ttgcagatcc tgccgcacct gaacggcccg ctgcacctgg ctccgatgac
     2581 cctgcgttgt accgtgggtt ctccgccgcg tcgtgtggac tgtaccattc atcgtccgtc
     2641 taacggcggc ctgatcgtag aactggaacc agcaaccaag accactaaca ttgcgccggc
     2701 tctggacggt gcgtttcatc gtatcacttc ttcatcctcc ctgatgggcc tgtgtgacga
     2761 aaccgcgact attatccgtg agattactgg ctacgaccgt gtgatggtag tacgtttcga
     2821 tgaagagggt aatggcgaaa ttctgtccga acgtcgtcgt gcggacctgg aagcgttcct
     2881 gggtaaccgc tacccggcgt ctactattcc gcagatcgct cgtcgcctgt acgaacataa
     2941 ccgtgttcgc ctgctggtag atgtgaacta tactccggtt ccgctacagc cgcgcatcag
     3001 cccgctgaac ggtcgtgatc tggatatgtc cctgtcttgc ctgcgctcta tgtccccgat
     3061 ccaccagaaa tacatgcagg acatgggcgt tggcgcgacc ctggtttgct ctctgatggt
     3121 gtctggtcgt ctgtggggtc tgatcgcttg ccaccactac gaaccgcgct tcgttccgtt
     3181 ccacattcgc gctgctggcg aagcgctggc ggaaacttgt gcgatccgca tcgcgacgct
     3241 ggagagcttt gcacagtctc agtccaaatg agcggccgcg actctagatc ataatcagcc
     3301 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3361 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3481 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     3541 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3601 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3661 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3721 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3781 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3841 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3901 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3961 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4021 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4081 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     4141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4381 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4441 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     4501 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4561 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4621 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4681 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4741 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4801 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4861 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4921 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4981 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     5041 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5101 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5161 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5221 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5281 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5341 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5401 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5461 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     5521 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     5581 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5641 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5701 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     5761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6541 ttgctcacat g
//
