LOCUS       sequence-218696-       12019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of ERK:MEK fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12019)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-ERK:MEK
FEATURES             Location/Qualifiers
     source          1..12019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1146..1181
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     protein_bind    complement(2433..2457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2474..2984
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2903..2921
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3000..3009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3006..3008,3009..3011,3012..3722)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3006..3008,3009..3011,3012..3722)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3051..3072)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3312..3331)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3659..3680
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3738..3797
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4866..5237
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    5263..5851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5316..5336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5734..5745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5854..5870)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5855..5871)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5999..6032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6052..6232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6270..6289)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6335..6390
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6389..6408)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6751..6767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6751..6767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6764..6786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6775..6791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6799..6805,6806..6823,6824..6829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6844..6865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6924..7119
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6970..7105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7125..7259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7162..7181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7216..7235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7327..7344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7498..8085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7578..7597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8256..9047,9048..9116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8879..8898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9117..9221)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9252..9631
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9633..9890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9937..10062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10554..10671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11031..11264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11449..11493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    11768..11788
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11815..11833
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11856..11874
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtt ctagtattag caaggccttt cctctatata aggaagttca
       61 tttcatttgg agaagatccc ccaagtttgt acaaaaaagc aggcttcacc atggcggcgg
      121 cggcggcggg cccggagatg gtccgcgggc aggtgttcga cgtggggccg cgctacacta
      181 atctctcgta catcggagaa ggcgcctacg gcatggtttg ttctgcttat gataatctca
      241 acaaagttcg agttgctatc aagaaaatca gtccttttga gcaccagacc tactgtcaga
      301 gaaccctgag agagataaaa atcctactgc gcttcagaca tgagaacatc atcggcatca
      361 atgacatcat ccgggcacca accattgagc agatgaaaga tgtatatata gtacaggacc
      421 tcatggagac agatctttac aagctcttga agacacagca cctcagcaat gatcatatct
      481 gctattttct ttatcagatc ctgagaggat taaagtatat acattcagct aatgttctgc
      541 accgtgacct caagccttcc aacctcctgc tgaacaccac ttgtgatctc aagatctgtg
      601 actttggcct tgcccgtgtt gcagatccag accatgatca tacagggttc ttgacagagt
      661 atgtagccac gcgttggtac agagctccag aaattatgtt gaattccaag ggttatacca
      721 agtccattga tatttggtct gtgggctgca tcctggcaga gatgctatcc aacaggccta
      781 tctttccagg aaagcattac cttgaccagc tgaatcacat cctgggtatt cttggatctc
      841 catcacagga agatctgaat tgtataataa atttaaaagc tagaaactat ttgctttctc
      901 tcccgcacaa aaataaggtg ccgtggaaca gattgttccc aaacgctgac tccaaagctc
      961 tggatttact ggataaaatg ttgacattta accctcacaa gaggattgaa gttgaacagg
     1021 ctctggccca cccgtacctg gagcagtatt atgacccaag tgatgagccc attgctgaag
     1081 caccattcaa gtttgacatg gagctggacg acttacctaa ggagaagctc aaagaactca
     1141 tttttgaaga gactgctcga ttccagccag gatacagatc tgaaggcgag ggtgaaggcg
     1201 agggtgaaag cggatccggc ggagttggaa gcgcgttacc cgggtccaaa atgcccaaga
     1261 agaagccgac gcccatccag ctgaacccgg cccccgacgg ctctgcagtt aacgggacca
     1321 gctctgcgga gaccaacttg gaggccgcgc agaagaaggc ggaggaggca gaggctgatg
     1381 agcagcagcg aaagcgcctt gaggcctttc ttacccagaa gcagaaggtg ggagaactga
     1441 aggatgacga ctttgagaag atcagtgagc tgggggctgg caatggcggt gtggtgttca
     1501 aggtctccca caagccttct ggcctggtca tggccagaaa gctaattcat ctggagatca
     1561 aacccgcaat ccggaaccag atcataaggg agctgcaggt tctgcatgag tgcaactctc
     1621 cgtacatcgt gggcttctat ggtgcgttct acagcgatgg cgagatcagt atctgcatgg
     1681 agcacatgga tggaggttct ctggatcaag tcctgaagaa agctggaaga attcctgaac
     1741 aaattttagg aaaagttagc attgctgtaa taaaaggcct gacatatctg agggagaagc
     1801 acaagatcat gcacagagat gtcaagccct ccaacatcct agtcaactcc cgtggggaga
     1861 tcaagctctg tgactttggg gtcagcgggc agctcatcga ctccatggcc aactccttcg
     1921 tgggcacaag gtcctacatg tcgccagaaa gactccaggg gactcattac tctgtgcagt
     1981 cagacatctg gagcatggga ctgtctctgg tagagatggc ggttgggagg tatcccatcc
     2041 ctcctccaga tgccaaggag ctggagctga tgtttgggtg ccaggtggaa ggagatgcgg
     2101 ctgagacccc acccaggcca aggacccccg ggaggcccct tagctcatac ggaatggaca
     2161 gccgacctcc catggcaatt tttgagttgt tggattacat agtcaacgag cctcctccaa
     2221 aactgcccag tggagtgttc agtctggaat ttcaagattt tgtgaataaa tgcttaataa
     2281 aaaaccccgc agagagagca gatttgaagc aactcatggt tcatgctttt atcaagagat
     2341 ctgatgctga ggaagtggat tttgcaggtt ggctctgctc caccatcggc cttaaccagc
     2401 ccagcacacc aacccatgct gctggcgtct aaacccagct ttcttgtaca aagtggtgat
     2461 aatcgaattc cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     2521 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     2581 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     2641 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     2701 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     2761 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     2821 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     2881 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     2941 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggtcg
     3001 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3061 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3121 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3181 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3241 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3301 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3361 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3421 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3481 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3541 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3601 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3661 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3721 aggctagcgg atcaggtcaa tgtactaact acgctttgtt gaagcttgcc ggcgatgttg
     3781 aaagtaaccc cggtcctgga tccatgtcca gactggacaa gagcaaagtc ataaacggcg
     3841 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     3901 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     3961 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     4021 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
     4081 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     4141 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     4201 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     4261 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     4321 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     4381 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc tcgagcgaat
     4441 tccagtacct gccagataca gacgatcgtc accggattga ggagaaacgt aaaaggacat
     4501 atgagacctt caagagcatc atgaagaaga gtcctttcag cggacccacc gacccccggc
     4561 ctccacctcg acgcattgct gtgccttccc gcagctcagc ttctgtcccc aagccagcac
     4621 cccagcccta tccctttacg tcatccctga gcaccatcaa ctatgatgag tttcccacca
     4681 tggtgtttcc ttctgggcag atcagccagg cctcggcctt ggccccggcc cctccccaag
     4741 tcctgcccca ggctccagcc cctgcccctg ctccagccat ggtatcagct ctggcccagg
     4801 ccccagcccc tgtcccagtc ctagccccag gccctcctca ggctgtggcc ccacctgccc
     4861 ccaagcccac ccaggctggg gaaggaacgc tgtcagaggc cctgctgcag ctgcagtttg
     4921 atgatgaaga cctgggggcc ttgcttggca acagcacaga cccagctgtg ttcacagacc
     4981 tggcatccgt cgacaactcc gagtttcagc agctgctgaa ccagggcata cctgtggccc
     5041 cccacacaac tgagcccatg ctgatggagt accctgaggc tataactcgc ctagtgacag
     5101 gggcccagag gccccccgac ccagctcctg ctccactggg ggccccgggg ctccccaatg
     5161 gcctcctttc aggagatgaa gacttctcct ccattgcgga catggacttc tcagccctgc
     5221 tgagtcagat cagctcctaa agatctgatc aagcttatcg ataatcaacc tctggattac
     5281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5641 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5821 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg aggttcgctt
     5881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5941 gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat
     6001 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc
     6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     6241 ttgaatcgcc agtgtgtcga gctagcagat ctttttccct ctgccaaaaa ttatggggac
     6301 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     6361 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     6421 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     6481 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     6541 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     6601 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     6661 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     6721 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6901 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     6961 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7021 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7081 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     7141 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7201 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     7261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7621 tcctgttccg accctgccgc ttacggatac ctgtccgcct ttctcccttc gggaagcgtg
     7681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8221 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8281 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8341 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8401 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8461 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8521 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8581 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8641 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8701 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8761 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8821 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8881 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8941 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9001 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9061 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9241 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     9301 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9361 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9481 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9541 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9601 catctacgta ttagtcatcg ctattaccat gggaggcgtg gcctgggcgg gactggggag
     9661 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatac tggcctgtta
    10201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10381 aagaaaaaag cacagcaagc agcaggatct tcagacctgg aaattcccta caatccccaa
    10441 agtcaaggag tagtagaatc tatgaataaa gaattaaaga aaattatagg acaggtaaga
    10501 gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca caattttaaa
    10561 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    10621 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    10681 tacagggaca gcagaaatcc actttggaaa ggaccagcaa agctcctctg gaaaggtgaa
    10741 ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag aaaagcaaag
    10801 atcattaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag tagacaggat
    10861 gaggattaga acatggaaaa gtttagtaaa acaccataag gaggagatat gagggacaat
    10921 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    10981 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    11041 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    11101 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    11161 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    11221 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    11281 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    11341 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    11401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    11461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    11521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    11581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    11641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    11701 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tctagtgatt
    11761 atcggatcaa ctttgtatag aaaagttggg tacaactccc actagacata tcctactcta
    11821 tcattgatag agtgaccctt ccatagacat atcctactct atcattgata gagtgaccct
    11881 tccactaggt tcacttttct ctatcattga tagggagtgg taaacaattg atggacaaaa
    11941 tctatcaatg atagagagtg catgttacta gtattgttct tctctatcat tgatagtgac
    12001 tttgcctaat cacttagca
//
