LOCUS       sequence-218699-        7182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7182)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..7182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2428..3120
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             3190..3882
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    4028..4149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4065..4084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4119..4138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4156..4611)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4293..4314)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4505..4524
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4638..4742
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4744..5101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4744..4935
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4767..4787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4952..5087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5136..5930
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5136..5930
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5190..5209)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5800..5819
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6118..6137)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6162..6209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7027..7046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tccatggcgg ccccccgccc gtctcccgcg
      721 atccccgttt cggtctcggc tccggctttt tacgccccgc agaagaagtt cggccctgtg
      781 gtggccccaa agcccaaagt gaatcccttc cggcccaggg acagcgagcc tcccccggca
      841 cccggggccc agcgcgcaca gatgggccgg gtgggcgata ttcccccgcc gcccccggaa
      901 gactttcccc tgcctccacc tccccttgct ggggatggcg acgatgcaga gggtgctctg
      961 ggaggtgcct tcccgccgcc ccctcccccg atcgaggaat catttccccc tgcgcctctg
     1021 gaggaggaga tcttcccttc cccgccgcct cctccggagg aggagggagg gcctgaggcc
     1081 cccataccgc ccccaccaca gcccagggag aaggtgagca gtattgattt ggagatcgac
     1141 tctctgtcct cactgctgga tgacatgacc aagaatgatc ctttcaaagc ccgggtgtca
     1201 tctggatatg tgcccccacc agtggccact ccattcagtt ccaagtccag taccaagcct
     1261 gcagccgggg gcacagcacc cctgcctcct tggaagtccc cttccagctc ccagcctctg
     1321 ccccaggttc cggctccggc tcagagccag acacagttcc atgttcagcc ccagccccag
     1381 cccaagcctc aggtccaact ccatgtccag tcccagaccc agcctgtgtc tttggctaac
     1441 acccagcccc gagggccccc agcctcatct ccggctccag cccctaagtt ttctccagtg
     1501 actcctaagt ttactcctgt ggcttccaag ttcagtcctg gagccccagg tggatctggg
     1561 tcacaaccaa atcaaaaatt ggggcacccc gaagctcttt ctgctggcac aggctcccct
     1621 caacctccca gcttcaccta tgcccagcag agggagaagc cccgagtgca ggagaagcag
     1681 caccccgtgc ccccaccggc tcagaaccaa aaccaggtgc gctcccctgg ggccccaggg
     1741 cccctgactc tgaaggaggt ggaggagctg gagcagctga cccagcagct aatgcaggac
     1801 atggagcatc ctcagaggca gaatgtggct gtcaacgaac tctgcggccg atgccatcaa
     1861 cccctggccc gggcgcagcc agccgtccgc gctctagggc agctgttcca catcgcctgc
     1921 ttcacctgcc accagtgtgc gcagcagctc cagggccagc agttctacag tctggagggg
     1981 gcgccgtact gcgagggctg ttacactgac accctggaga agtgtaacac ctgcggggag
     2041 cccatcactg accgcatgct gagggccacg ggcaaggcct atcacccgca ctgcttcacc
     2101 tgtgtggtct gcgcccgccc cctggagggc acctccttca tcgtggacca ggccaaccgg
     2161 ccccactgtg tccccgacta ccacaagcag tacgccccga ggtgctccgt ctgctctgag
     2221 cccatcatgc ctgagcctgg ccgagatgag actgtgcgag tggtcgccct ggacaagaac
     2281 ttccacatga agtgttacaa gtgtgaggac tgcgggaagc ccctgtcgat tgaggcagat
     2341 gacaatggct gcttccccct ggacggtcac gtgctctgtc ggaagtgcca cactgctaga
     2401 gcccagacgg atccaccggt cgccaccatg agcgagctga tcaaggagaa catgcacatg
     2461 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
     2521 ggcaagccct acgagggcac ccagaccatg aagatcaagg tggtcgaggg cggccctctc
     2581 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaagc cttcatcaac
     2641 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
     2701 agaatcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcttccag
     2761 aacggctgca tcatctacaa cgtcaagatc aacggggtga acttcccatc caacggccct
     2821 gtgatgcaga agaaaacacg cggctgggag gccaacaccg agatgctgta ccccgctgac
     2881 ggcggcctga gaggccacag ccagatggcc ctgaagctcg tgggcggggg ctacctgcac
     2941 tgctccttca agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     3001 ttccacttcg tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc
     3061 gagcagcacg agatggctgt ggccaagtac tgcgacctcc ctagcaaact ggggcacaga
     3121 gggcatggca ccggcagcac cggcagcggc agctccggta ccgcctcctc cgaggacaac
     3181 aacatggcca tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc
     3241 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     3301 acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     3361 ctggctacca gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc
     3421 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa
     3481 gacgggggcg tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac
     3541 aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     3601 cgcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac
     3661 agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca
     3721 tacagatcca agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac
     3781 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct
     3841 gtggccaagt actgcgacct ccctagcaaa ctggggcaca gagatgaatc cggactcaga
     3901 tcttgagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     3961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4141 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4201 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4261 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4321 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4381 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4441 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4501 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4561 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     4621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4741 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4921 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4981 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5041 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5101 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5281 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     5341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5641 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     5701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     6121 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6181 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6241 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6301 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6361 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     6421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6661 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
