LOCUS       sequence-218701-        7882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7882)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..7882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3885..3894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3891..4601
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4036..4054)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4304..4323
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4724..4845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4761..4780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4815..4834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4852..5307)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4989..5010)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5201..5220
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5334..5438
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5440..5797
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5440..5631
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5463..5483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5648..5783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5832..6626
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5832..6626
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5886..5905)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6496..6515
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6814..6833)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6858..6905
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7234..7822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7723..7742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccaccatg gggagccgga
      721 cgccagagtc ccctctccac gccgtgcagc tgcgctgggg cccccggcgc cgacccccgc
      781 tgctgccgct gctgttgctg ctgctgccgc cgccacccag ggtcgggggc ttcaacttag
      841 acgcggaggc cccagcagta ctctcggggc ccccgggctc cttcttcgga ttctcagtgg
      901 agttttaccg gccgggaaca gacggggtca gtgtgctggt gggagcaccc aaggctaata
      961 ccagccagcc aggagtgctg cagggtggtg ctgtctacct ctgtccttgg ggtgccagcc
     1021 ccacacagtg cacccccatt gaatttgaca gcaaaggctc tcggctcctg gagtcctcac
     1081 tgtccagctc agagggagag gagcctgtgg agtacaagtc cttgcagtgg ttcggggcaa
     1141 cagttcgagc ccatggctcc tccatcttgg catgcgctcc actgtacagc tggcgcacag
     1201 agaaggagcc actgagcgac cccgtgggca cctgctacct ctccacagat aacttcaccc
     1261 gaattctgga gtatgcaccc tgccgctcag atttcagctg ggcagcagga cagggttact
     1321 gccaaggagg cttcagtgcc gagttcacca agactggccg tgtggtttta ggtggaccag
     1381 gaagctattt ctggcaaggc cagatcctgt ctgccactca ggagcagatt gcagaatctt
     1441 attaccccga gtacctgatc aacctggttc aggggcagct gcagactcgc caggccagtt
     1501 ccatctatga tgacagctac ctaggatact ctgtggctgt tggtgaattc agtggtgatg
     1561 acacagaaga ctttgttgct ggtgtgccca aagggaacct cacttacggc tatgtcacca
     1621 tccttaatgg ctcagacatt cgatccctct acaacttctc aggggaacag atggcctcct
     1681 actttggcta tgcagtggcc gccacagacg tcaatgggga cgggctggat gacttgctgg
     1741 tgggggcacc cctgctcatg gatcggaccc ctgacgggcg gcctcaggag gtgggcaggg
     1801 tctacgtcta cctgcagcac ccagccggca tagagcccac gcccaccctt accctcactg
     1861 gccatgatga gtttggccga tttggcagct ccttgacccc cctgggggac ctggaccagg
     1921 atggctacaa tgatgtggcc atcggggctc cctttggtgg ggagacccag cagggagtag
     1981 tgtttgtatt tcctgggggc ccaggagggc tgggctctaa gccttcccag gttctgcagc
     2041 ccctgtgggc agccagccac accccagact tctttggctc tgcccttcga ggaggccgag
     2101 acctggatgg caatggatat cctgatctga ttgtggggtc ctttggtgtg gacaaggctg
     2161 tggtatacag gggccgcccc atcgtgtccg ctagtgcctc cctcaccatc ttccccgcca
     2221 tgttcaaccc agaggagcgg agctgcagct tagaggggaa ccctgtggcc tgcatcaacc
     2281 ttagcttctg cctcaatgct tctggaaaac acgttgctga ctccattggt ttcacagtgg
     2341 aacttcagct ggactggcag aagcagaagg gaggggtacg gcgggcactg ttcctggcct
     2401 ccaggcaggc aaccctgacc cagaccctgc tcatccagaa tggggctcga gaggattgca
     2461 gagagatgaa gatctacctc aggaacgagt cagaatttcg agacaaactc tcgccgattc
     2521 acatcgctct caacttctcc ttggaccccc aagccccagt ggacagccac ggcctcaggc
     2581 cagccctaca ttatcagagc aagagccgga tagaggacaa ggctcagatc ttgctggact
     2641 gtggagaaga caacatctgt gtgcctgacc tgcagctgga agtgtttggg gagcagaacc
     2701 atgtgtacct gggtgacaag aatgccctga acctcacttt ccatgcccag aatgtgggtg
     2761 agggtggcgc ctatgaggct gagcttcggg tcaccgcccc tccagaggct gagtactcag
     2821 gactcgtcag acacccaggg aacttctcca gcctgagctg tgactacttt gccgtgaacc
     2881 agagccgcct gctggtgtgt gacctgggca accccatgaa ggcaggagcc agtctgtggg
     2941 gtggccttcg gtttacagtc cctcatctcc gggacactaa gaaaaccatc cagtttgact
     3001 tccagatcct cagcaagaat ctcaacaact cgcaaagcga cgtggtttcc tttcggctct
     3061 ccgtggaggc tcaggcccag gtcaccctga acggtgtctc caagcctgag gcagtgctat
     3121 tcccagtaag cgactggcat ccccgagacc agcctcagaa ggaggaggac ctgggacctg
     3181 ctgtccacca tgtctatgag ctcatcaacc aaggccccag ctccattagc cagggtgtgc
     3241 tggaactcag ctgtccccag gctctggaag gtcagcagct cctatatgtg accagagtta
     3301 cgggactcaa ctgcaccacc aatcacccca ttaacccaaa gggcctggag ttggatcccg
     3361 agggttccct gcaccaccag caaaaacggg aagctccaag ccgcagctct gcttcctcgg
     3421 gacctcagat cctgaaatgc ccggaggctg agtgtttcag gctgcgctgt gagctcgggc
     3481 ccctgcacca acaagagagc caaagtctgc agttgcattt ccgagtctgg gccaagactt
     3541 tcttgcagcg ggagcaccag ccatttagcc tgcagtgtga ggctgtgtac aaagccctga
     3601 agatgcccta ccgaatcctg cctcggcagc tgccccaaaa agagcgtcag gtggccacag
     3661 ctgtgcaatg gaccaaggca gaaggcagct atggcgtccc actgtggatc atcatcctag
     3721 ccatcctgtt tggcctcctg ctcctaggtc tactcatcta catcctctac aagcttggat
     3781 tcttcaaacg ctccctccca tatggcaccg ccatggaaaa agctcagctc aagcctccag
     3841 ccacctctga tgccacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
     3901 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3961 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4021 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     4081 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     4141 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4201 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     4261 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     4321 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     4381 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     4441 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     4501 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4561 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     4621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     4861 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4921 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4981 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5041 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5101 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5161 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5221 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5281 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     5461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5761 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     5821 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5881 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5941 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     6001 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6061 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6121 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6181 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6241 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6301 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     6361 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6421 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6481 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6541 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6601 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6781 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     6841 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     6901 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6961 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     7021 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     7081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7861 ttgctggcct tttgctcaca tg
//
