LOCUS       sequence-218702-        6057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-VASP-5
FEATURES             Location/Qualifiers
     source          1..6057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1815..2774
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    2899..3020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2936..2955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2990..3009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3027..3482)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3164..3185)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3376..3395
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3509..3613
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3615..3972
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3615..3806
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3638..3658)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3823..3958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4007..4801
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4007..4801
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4061..4080)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4671..4690
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4989..5008)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5033..5080
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5409..5997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5898..5917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gatgccacca
      661 tgagcgagac ggtcatctgt tccagccggg ctactgtgat gctttatgat gacagcaaca
      721 agcgatggct ccctgctggc actggtcccc aggccttcag ccgcgtccag atataccaca
      781 accccactgc taactccttc cgagttgttg gccgcaagat gcagccggat cagcaggtgg
      841 ttatcaactg tgccatcatt cggggtgtca agtacaatca ggccactccc atcttccatc
      901 agtggcgaga tgcccgccag gtctggggcc tcaacttcgg cagcaaggag gacgccatac
      961 agtttgcaac aggcatggcc aacgccctag aggccttgga aggaggtggg cctcccccag
     1021 ccccagcacc ccctgcctgg tctgcccaga atggtccctc cccagaggag ctggaacaac
     1081 agaaaaggca gccggagcat atggagcgcc gggtctccaa tgcaggaggc ccacctgctc
     1141 ccccagctgg gggccctcct ccacctccag gacctccccc tcctccaggt ccccccccac
     1201 ccccaggtct gccctcctca ggggtatctg gggcaggtca tggagcaggg gcagccccac
     1261 cccctgcacc cccactccct acaacacagg gccccaatag tgggggttcc ggggccccag
     1321 gcctggctgc tgccattgct ggagccaaac tcaggaaagt gagcaagcag gaggaggcct
     1381 ctggggggcc cctggccccc aaagctgaga acagtcgaag cactggtggg gggcttatgg
     1441 aagagatgaa cgccatgctg gcccggagaa gaaaagccac acaggttggg gagaagcccc
     1501 ccaaagacga gtcagccagt gaggagtcag aggcccgact ccctgcccag agtgaacctg
     1561 tgagaagacc ctgggagaag aacagcacaa ccttgccaag gatgaagtcg tcctcttctg
     1621 tgactacctc cgaggcccac ccctcgacgc cctgctccag tgatgactcc gacttggaga
     1681 gggtgaagca ggagcttctg gaagaggtgc ggaaggagct acagaaaatg aaagaggaaa
     1741 tcatcgaagt ctttgtccag gagctgagga agcggggttc tcctagctcc ggaggggatc
     1801 caccggtcgc caccatgtcg gtaccgctga ctacctcagc attcggccac gcgtttctgg
     1861 ctaactgtga acgcgagcag atccacctgg cgggctccat tcagccgcac ggtatcctgc
     1921 tggctgtgaa agagccggac aacgtggtga tccaggcttc tattaacgct gcggagttcc
     1981 tgaacaccaa ctctgttgtt ggccgtccgc tgcgtgacct gggcggcgat ctgcctttgc
     2041 agatcctgcc gcacctgaac ggcccgctgc acctggctcc gatgaccctg cgttgtaccg
     2101 tgggttctcc gccgcgtcgt gtggactgta ccattcatcg tccgtctaac ggcggcctga
     2161 tcgtagaact ggaaccagca accaagacca ctaacattgc gccggctctg gacggtgcgt
     2221 ttcatcgtat cacttcttca tcctccctga tgggcctgtg tgacgaaacc gcgactatta
     2281 tccgtgagat tactggctac gaccgtgtga tggtagtacg tttcgatgaa gagggtaatg
     2341 gcgaaattct gtccgaacgt cgtcgtgcgg acctggaagc gttcctgggt aaccgctacc
     2401 cggcgtctac tattccgcag atcgctcgtc gcctgtacga acataaccgt gttcgcctgc
     2461 tggtagatgt gaactatact ccggttccgc tacagccgcg catcagcccg ctgaacggtc
     2521 gtgatctgga tatgtccctg tcttgcctgc gctctatgtc cccgatccac cagaaataca
     2581 tgcaggacat gggcgttggc gcgaccctgg tttgctctct gatggtgtct ggtcgtctgt
     2641 ggggtctgat cgcttgccac cactacgaac cgcgcttcgt tccgttccac attcgcgctg
     2701 ctggcgaagc gctggcggaa acttgtgcga tccgcatcgc gacgctggag agctttgcac
     2761 agtctcagtc caaatgagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     2821 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2881 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3001 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3061 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3241 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3301 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3361 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3421 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3481 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3541 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3601 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3661 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3721 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3781 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3841 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3901 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3961 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4021 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4081 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4141 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4201 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4261 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4321 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4381 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4441 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4501 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4561 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4621 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4681 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4741 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4801 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4981 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5041 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5101 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5161 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5221 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5521 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg
//
