LOCUS       sequence-218710-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Ngn2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Ngn2
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    48..66
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    89..107
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    253..271
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    333..357
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1155..1179)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1196..1706
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1625..1643
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1722..1731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1728..1730,1731..1733,1734..2444)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1728..1730,1731..1733,1734..2444)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1773..1794)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2034..2053)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2381..2402
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2460..2519
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3588..3959
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3985..4573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4038..4058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4456..4467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4576..4592)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4577..4593)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4721..4754)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4774..4954
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4992..5011)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5057..5112
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5111..5130)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5473..5489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5473..5489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5486..5508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5497..5513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5521..5527,5528..5545,5546..5551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5566..5587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5646..5841
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5692..5827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5847..5981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5884..5903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5938..5957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6049..6066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6220..6807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6300..6319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6978..7769,7770..7838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7601..7620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7839..7943)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7974..8353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8355..8612
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8659..8784
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9276..9393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9753..9986
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10171..10215
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caactttgta tagaaaagtt gggtacaact cccactagac atatcctact ctatcattga
       61 tagagtgacc cttccataga catatcctac tctatcattg atagagtgac ccttccacta
      121 ggttcacttt tctctatcat tgatagggag tggtaaacaa ttgatggaca aaatctatca
      181 atgatagaga gtgcatgtta ctagtattgt tcttctctat cattgatagt gactttgcct
      241 aatcacttag caactctatc attgatagag ttctagtatt agcaaggcct ttcctctata
      301 taaggaagtt catttcattt ggagaagatc ccccaagttt gtacaaaaaa gcaggcttca
      361 ccatgttcgt caaatctgag actctggagt tgaaggagga agaggaggta ctgatgctgc
      421 tgggctcggc ttccccggcc tcggcgaccc tgaccccgat gtcctccagc gcggacgagg
      481 aggaggacga ggagctgcgc cggccgggct ccgcgcgtgg gcagcgtgga gcggaagccg
      541 ggcagggggt gcagggcagt ccggcgtcgg gtgccggggg ttgccggcca gggcggctgc
      601 tgggcctgat gcacgagtgc aagcgtcgcc cgtcgcgctc acgggccgtc tcccgaggtg
      661 ccaagacggc ggagacggtg cagcgcatca agaagacccg caggctcaag gccaacaacc
      721 gcgagcgcaa ccgcatgcac aacctaaacg ccgcgctgga cgcgctgcgc gaggtgctgc
      781 ccaccttccc cgaggatgcc aagctcacga agatcgagac gctgcgcttc gcccacaatt
      841 acatctgggc gctcaccgag actctgcgcc tggcggacca ctgcgccggc gccggtggcc
      901 tccagggggc gctcttcacg gaggcggtgc tcctgagccc gggagctgcg ctcggcgcca
      961 gcggggacag cccttctcca ccttcctcct ggagctgcac caacagcccg gcgtcatcct
     1021 ccaactccac gtccccatac agctgcactt tatcgcccgc tagccccggg tcagacgtgg
     1081 actactggca gcccccacct ccggagaagc atcgttatgc gcctcacctg cccctcgcca
     1141 gggactgtat ctagacccag ctttcttgta caaagtggtg ataatcgaat tccacggggt
     1201 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1261 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1321 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1381 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1441 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1501 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     1561 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     1621 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     1681 accgaatcac cgacctctct ccccaggggg atccaccggt cgccaccatg gtgagcaagg
     1741 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1801 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1861 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1921 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1981 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2041 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2101 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2161 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2221 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2281 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2341 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2401 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggctagc ggatcaggtc
     2461 aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt tgaaagtaac cccggtcctg
     2521 gatccatgtc cagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg
     2581 gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     2641 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     2701 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     2761 tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag
     2821 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     2881 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     2941 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     3001 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     3061 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     3121 aacagctaaa gtgcgaaagc ggcgggccgg cctcgagcga attccagtac ctgccagata
     3181 cagacgatcg tcaccggatt gaggagaaac gtaaaaggac atatgagacc ttcaagagca
     3241 tcatgaagaa gagtcctttc agcggaccca ccgacccccg gcctccacct cgacgcattg
     3301 ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc accccagccc tatcccttta
     3361 cgtcatccct gagcaccatc aactatgatg agtttcccac catggtgttt ccttctgggc
     3421 agatcagcca ggcctcggcc ttggccccgg cccctcccca agtcctgccc caggctccag
     3481 cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagcc cctgtcccag
     3541 tcctagcccc aggccctcct caggctgtgg ccccacctgc ccccaagccc acccaggctg
     3601 gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa gacctggggg
     3661 ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc gtcgacaact
     3721 ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca actgagccca
     3781 tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag aggccccccg
     3841 acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt tcaggagatg
     3901 aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag atcagctcct
     3961 aaagatctga tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4381 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4561 gccgcctccc cgcatcgata ccgtcgacct cgaggttcgc tttaagacca atgacttaca
     4621 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4681 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     4741 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4921 agaccctttt agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc
     4981 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     5041 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     5101 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     5161 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     5221 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5281 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5341 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5401 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     5461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     5641 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5821 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     5881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5941 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     6001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6361 gcttacggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6421 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6481 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6541 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6601 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6661 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6721 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6781 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6841 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6901 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6961 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7021 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7081 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7141 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7201 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7261 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7321 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7381 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7441 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7501 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7561 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7621 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7681 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7741 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7801 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7861 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7921 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     7981 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     8041 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     8101 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     8161 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     8221 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     8281 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     8341 cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     8401 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     8461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8581 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8641 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8701 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8761 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8821 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8881 agcagggagc tagaacgatt cgcagttaat actggcctgt tagaaacatc agaaggctgt
     8941 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9001 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9061 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa
     9121 gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg agtagtagaa
     9181 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     9241 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9301 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9361 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat
     9421 ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     9481 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag ggattatgga
     9541 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta gaacatggaa
     9601 aagtttagta aaacaccata aggaggagat atgagggaca attggagaag tgaattatat
     9661 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9721 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9781 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9841 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9901 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9961 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    10021 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    10081 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    10141 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    10201 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    10261 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    10321 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    10381 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    10441 tccattcgat tagtgaacgg atctcgacgg tatctagtga ttatcggat
//
