LOCUS       sequence-218733-       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Neurotrophin 3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-NT3
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    48..66
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    89..107
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    253..271
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    333..357
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1140..1164)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1181..1691
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1610..1628
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1707..1716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1713..1715,1716..1718,1719..2429)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1713..1715,1716..1718,1719..2429)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1758..1779)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2019..2038)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2366..2387
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2445..2504
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3573..3944
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3970..4558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4023..4043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4441..4452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4561..4577)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4562..4578)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4706..4739)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4759..4939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4977..4996)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5042..5097
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5096..5115)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5458..5474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5458..5474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5471..5493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5482..5498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5506..5512,5513..5530,5531..5536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5551..5572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5631..5826
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5677..5812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5832..5966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5869..5888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5923..5942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6034..6051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6205..6792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6285..6304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6963..7754,7755..7823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7586..7605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7824..7928)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7959..8338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8340..8597
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8644..8769
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9261..9378
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9738..9971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10156..10200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caactttgta tagaaaagtt gggtacaact cccactagac atatcctact ctatcattga
       61 tagagtgacc cttccataga catatcctac tctatcattg atagagtgac ccttccacta
      121 ggttcacttt tctctatcat tgatagggag tggtaaacaa ttgatggaca aaatctatca
      181 atgatagaga gtgcatgtta ctagtattgt tcttctctat cattgatagt gactttgcct
      241 aatcacttag caactctatc attgatagag ttctagtatt agcaaggcct ttcctctata
      301 taaggaagtt catttcattt ggagaagatc ccccaagttt gtacaaaaaa gcaggcttca
      361 ccatgtccat cttgttttat gtgatatttc ttgcttatct ccgtggcatc caaggcaaca
      421 gcatggatca aaggagtttg ccggaagact ctctcaattc cctcatcatc aagctgatcc
      481 aggcggatat cttgaaaaac aagctttcca aacagatggt ggatgttaag gaaaattacc
      541 agagcaccct gcccaaagca gaggcaccca gggaaccaga gcagggagag gccaccaggt
      601 cagagttcca gccaatgatt gcaacggaca cagagctact acggcaacag agacgctaca
      661 attcgccccg ggtcctgctg agtgacagca cccctttgga gccccctccc ttatacctaa
      721 tggaggatta tgtgggcaac ccggtggtag ccaatagaac ctcaccacgg aggaaacgct
      781 atgcagaaca taagagtcac cgaggagagt actcagtgtg tgacagtgag agcctgtggg
      841 tgaccgacaa gtcctcagcc attgacattc ggggacacca ggtcacagtg ctgggggaga
      901 tcaaaaccgg taactctcct gtgaaacaat atttttatga aacgagatgt aaagaagcca
      961 ggccggtcaa aaacggttgc agggggattg atgacaaaca ctggaactct cagtgcaaaa
     1021 cttcgcaaac ctatgtccga gcactgactt cagaaaacaa caaactcgta ggctggcgct
     1081 ggatacgaat agacacttcc tgtgtgtgtg ccttgtcgag aaaaattgga agaacatgaa
     1141 cccagctttc ttgtacaaag tggtgataat cgaattccac ggggttgggg ttgcgccttt
     1201 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     1261 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     1321 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     1381 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     1441 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     1501 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     1561 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     1621 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     1681 tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     1741 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1801 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1861 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1921 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1981 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2041 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2101 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2161 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2221 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2281 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2341 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2401 tcactctcgg catggacgag ctgtacaagg ctagcggatc aggtcaatgt actaactacg
     2461 ctttgttgaa gcttgccggc gatgttgaaa gtaaccccgg tcctggatcc atgtccagac
     2521 tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc ggtatcgaag
     2581 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     2641 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     2701 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     2761 agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     2821 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     2881 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     2941 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     3001 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     3061 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     3121 aaagcggcgg gccggcctcg agcgaattcc agtacctgcc agatacagac gatcgtcacc
     3181 ggattgagga gaaacgtaaa aggacatatg agaccttcaa gagcatcatg aagaagagtc
     3241 ctttcagcgg acccaccgac ccccggcctc cacctcgacg cattgctgtg ccttcccgca
     3301 gctcagcttc tgtccccaag ccagcacccc agccctatcc ctttacgtca tccctgagca
     3361 ccatcaacta tgatgagttt cccaccatgg tgtttccttc tgggcagatc agccaggcct
     3421 cggccttggc cccggcccct ccccaagtcc tgccccaggc tccagcccct gcccctgctc
     3481 cagccatggt atcagctctg gcccaggccc cagcccctgt cccagtccta gccccaggcc
     3541 ctcctcaggc tgtggcccca cctgccccca agcccaccca ggctggggaa ggaacgctgt
     3601 cagaggccct gctgcagctg cagtttgatg atgaagacct gggggccttg cttggcaaca
     3661 gcacagaccc agctgtgttc acagacctgg catccgtcga caactccgag tttcagcagc
     3721 tgctgaacca gggcatacct gtggcccccc acacaactga gcccatgctg atggagtacc
     3781 ctgaggctat aactcgccta gtgacagggg cccagaggcc ccccgaccca gctcctgctc
     3841 cactgggggc cccggggctc cccaatggcc tcctttcagg agatgaagac ttctcctcca
     3901 ttgcggacat ggacttctca gccctgctga gtcagatcag ctcctaaaga tctgatcaag
     3961 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4141 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4201 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4261 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4321 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4381 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4441 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4501 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4561 cgataccgtc gacctcgagg ttcgctttaa gaccaatgac ttacaaggca gctgtagatc
     4621 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4681 aagatgggat caattcacca tgggaataac ttcgtatagc atacattata cgaagttatg
     4741 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4801 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4861 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4921 gtgtggaaaa tctctagcag catctagttg aatcgccagt gtgtcgagct agcagatctt
     4981 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5041 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     5101 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     5161 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     5221 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     5281 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     5341 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     5401 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     5461 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5521 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5581 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     5641 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5701 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5761 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5821 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5941 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     6001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta cggatacctg
     6361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7921 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     7981 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8041 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8101 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8161 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     8221 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     8281 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
     8341 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8401 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8461 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8521 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8581 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8641 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8701 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8761 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8821 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8881 cgattcgcag ttaatactgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8941 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9001 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9061 aagatagagg aagagcaaaa caaaagtaag aaaaaagcac agcaagcagc aggatcttca
     9121 gacctggaaa ttccctacaa tccccaaagt caaggagtag tagaatctat gaataaagaa
     9181 ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa
     9241 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9301 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9361 acaaaaattc aaaattttcg ggtttattac agggacagca gaaatccact ttggaaagga
     9421 ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata
     9481 aaagtagtgc caagaagaaa agcaaagatc attagggatt atggaaaaca gatggcaggt
     9541 gatgattgtg tggcaagtag acaggatgag gattagaaca tggaaaagtt tagtaaaaca
     9601 ccataaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9661 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9721 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9781 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9841 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9901 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9961 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10021 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10081 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10141 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10201 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    10261 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    10321 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    10381 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    10441 aacggatctc gacggtatct agtgattatc ggat
//
