LOCUS       sequence-218734-       10771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the NeuroD1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10771)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-NeuroD1
FEATURES             Location/Qualifiers
     source          1..10771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1166..1190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1207..1717
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1636..1654
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1733..1742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1784..1805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2045..2064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2392..2413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2471..2530
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3599..3970
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3996..4584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4049..4069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4467..4478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4587..4603)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4588..4604)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4732..4765)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4785..4965
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5003..5022)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5068..5123
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5122..5141)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5484..5500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5484..5500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5497..5519)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5508..5524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5532..5538,5539..5556,5557..5562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5577..5598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5657..5852
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5703..5838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5858..5992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5895..5914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5949..5968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6060..6077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6231..6818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6311..6330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6989..7780,7781..7849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7612..7631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7850..7954)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7985..8364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8366..8623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8670..8795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9287..9404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9764..9997
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10182..10226
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10501..10521
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10548..10566
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10589..10607
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10753..10771
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 tctagtatta gcaaggcctt tcctctatat aaggaagttc atttcatttg gagaagatcc
       61 cccaagtttg tacaaaaaag caggcttcac catgaccaaa tcatacagcg agagcgggct
      121 gatgggcgag cctcagcccc aaggtccccc aagctggaca gatgagtgtc tcagttctca
      181 ggacgaggaa cacgaggcag acaagaaaga ggacgagctt gaagccatga atgcagagga
      241 ggactctctg agaaacgggg gagaggagga ggaggaagat gaggatctag aggaagagga
      301 ggaagaagaa gaggaggagg aggatcaaaa gcccaagaga cggggtccca aaaagaaaaa
      361 gatgaccaag gcgcgcctag aacgttttaa attaaggcgc atgaaggcca acgcccgcga
      421 gcggaaccgc atgcacgggc tgaacgcggc gctggacaac ctgcgcaagg tggtaccttg
      481 ctactccaag acccagaaac tgtctaaaat agagacactg cgcttggcca agaactacat
      541 ctgggctctg tcagagatcc tgcgctcagg caaaagccct gatctggtct ccttcgtaca
      601 gacgctctgc aaaggtttgt cccagcccac taccaatttg gtcgccggct gcctgcagct
      661 caaccctcgg actttcttgc ctgagcagaa cccggacatg cccccgcatc tgccaaccgc
      721 cagcgcttcc ttcccggtgc atccctactc ctaccagtcc cctggactgc ccagcccgcc
      781 ctacggcacc atggacagct cccacgtctt ccacgtcaag ccgccgccac acgcctacag
      841 cgcagctctg gagcccttct ttgaaagccc cctaactgac tgcaccagcc cttcctttga
      901 cggacccctc agcccgccgc tcagcatcaa tggcaacttc tctttcaaac acgaaccatc
      961 cgccgagttt gaaaaaaatt atgcctttac catgcactac cctgcagcga cgctggcagg
     1021 gccccaaagc cacggatcaa tcttctcttc cggtgccgct gcccctcgct gcgagatccc
     1081 catagacaac attatgtctt tcgatagcca ttcgcatcat gagcgagtca tgagtgccca
     1141 gcttaatgcc atctttcacg attagaccca gctttcttgt acaaagtggt gataatcgaa
     1201 ttccacgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     1261 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     1321 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     1381 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     1441 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     1501 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     1561 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     1621 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     1681 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg tcgccaccat
     1741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1801 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2041 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2161 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2221 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2341 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggctag
     2461 cggatcaggt caatgtacta actacgcttt gttgaagctt gccggcgatg ttgaaagtaa
     2521 ccccggtcct ggatccatgt ccagactgga caagagcaaa gtcataaacg gcgctctgga
     2581 attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     2641 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     2701 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     2761 gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga
     2821 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     2881 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     2941 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     3001 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     3061 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     3121 tggcctggag aaacagctaa agtgcgaaag cggcgggccg gcctcgagcg aattccagta
     3181 cctgccagat acagacgatc gtcaccggat tgaggagaaa cgtaaaagga catatgagac
     3241 cttcaagagc atcatgaaga agagtccttt cagcggaccc accgaccccc ggcctccacc
     3301 tcgacgcatt gctgtgcctt cccgcagctc agcttctgtc cccaagccag caccccagcc
     3361 ctatcccttt acgtcatccc tgagcaccat caactatgat gagtttccca ccatggtgtt
     3421 tccttctggg cagatcagcc aggcctcggc cttggccccg gcccctcccc aagtcctgcc
     3481 ccaggctcca gcccctgccc ctgctccagc catggtatca gctctggccc aggccccagc
     3541 ccctgtccca gtcctagccc caggccctcc tcaggctgtg gccccacctg cccccaagcc
     3601 cacccaggct ggggaaggaa cgctgtcaga ggccctgctg cagctgcagt ttgatgatga
     3661 agacctgggg gccttgcttg gcaacagcac agacccagct gtgttcacag acctggcatc
     3721 cgtcgacaac tccgagtttc agcagctgct gaaccagggc atacctgtgg ccccccacac
     3781 aactgagccc atgctgatgg agtaccctga ggctataact cgcctagtga caggggccca
     3841 gaggcccccc gacccagctc ctgctccact gggggccccg gggctcccca atggcctcct
     3901 ttcaggagat gaagacttct cctccattgc ggacatggac ttctcagccc tgctgagtca
     3961 gatcagctcc taaagatctg atcaagctta tcgataatca acctctggat tacaaaattt
     4021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4381 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4561 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgaggttcg ctttaagacc
     4621 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4681 agggctaatt cactcccaac gaagacaaga tgggatcaat tcaccatggg aataacttcg
     4741 tatagcatac attatacgaa gttatgctgc tttttgcttg tactgggtct ctctggttag
     4801 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4861 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4921 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagttgaatc
     4981 gccagtgtgt cgagctagca gatctttttc cctctgccaa aaattatggg gacatcatga
     5041 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5101 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5161 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5221 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     5281 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     5341 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     5401 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     5461 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5521 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5581 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5641 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5701 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5761 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5821 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     5881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     6001 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6361 ccgaccctgc cgcttacgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7021 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7081 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7381 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7441 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7921 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7981 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     8041 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     8101 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     8161 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     8221 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     8281 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     8341 gtattagtca tcgctattac catgggaggc gtggcctggg cgggactggg gagtggcgag
     8401 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     8461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8641 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8701 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8761 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8821 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8881 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat
     8941 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9001 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9061 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa
     9121 aagcacagca agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag
     9181 gagtagtaga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     9241 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9301 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9361 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9421 acagcagaaa tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     9481 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta
     9541 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     9601 agaacatgga aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa
     9661 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     9721 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     9781 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9841 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9901 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9961 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    10021 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    10081 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    10141 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    10201 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    10261 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    10321 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    10381 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    10441 acagagacag atccattcga ttagtgaacg gatctcgacg gtatctagtg attatcggat
    10501 caactttgta tagaaaagtt gggtacaact cccactagac atatcctact ctatcattga
    10561 tagagtgacc cttccataga catatcctac tctatcattg atagagtgac ccttccacta
    10621 ggttcacttt tctctatcat tgatagggag tggtaaacaa ttgatggaca aaatctatca
    10681 atgatagaga gtgcatgtta ctagtattgt tcttctctat cattgatagt gactttgcct
    10741 aatcacttag caactctatc attgatagag t
//
