LOCUS       sequence-218735-       11440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of a dominant negative form of the TCF4 
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCWXPGR-pTF-dnTCF4
FEATURES             Location/Qualifiers
     source          1..11440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(1741..1765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1782..2292
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2211..2229
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2314..2316,2317..2319,2320..3030)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2314..2316,2317..2319,2320..3030)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2359..2380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2620..2639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2967..2988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3046..3105
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4174..4545
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4571..5159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4624..4644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5042..5053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5162..5178)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5179)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5307..5340)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5360..5540
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5578..5597)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5643..5698
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5697..5716)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6059..6075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6059..6075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6072..6094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6083..6099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6107..6113,6114..6131,6132..6137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6152..6173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6232..6427
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6278..6413
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6433..6567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6470..6489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6524..6543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6635..6652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6806..7393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6886..6905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7564..8355,8356..8424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8187..8206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8425..8529)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8560..8939
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8941..9198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9245..9370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9862..9979
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10339..10572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10757..10801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    11076..11096
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11123..11141
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11164..11182
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11328..11346
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11408..11432
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactacaaag acgatgacga taaaaaggat ccgagctcga attccgaaaa ctcctcggca
       61 gagagggatt tagctgatgt caaatcgtct ctagtcaatg aatcagaaac gaatcaaaac
      121 agctcctccg attccgaggc ggaaagacgg cctccgcctc gctccgaaag tttccgagac
      181 aaatcccggg aaagtttgga agaagcggcc aagaggcaag atggagggct ctttaagggg
      241 ccaccgtatc ccggctaccc cttcatcatg atccccgacc tgacgagccc ctacctcccc
      301 aacggatcgc tctcgcccac cgcccgaacc tatctccaga tgaaatggcc actgcttgat
      361 gtccaggcag ggagcctcca gagtagacaa gccctcaagg atgcccggtc cccatcaccg
      421 gcacacattg tctctaacaa agtgccagtg gtgcagcacc ctcaccatgt ccaccccctc
      481 acgcctctta tcacgtacag caatgaacac ttcacgccgg gaaacccacc tccacactta
      541 ccagccgacg tagaccccaa aacaggaatc ccacggcctc cgcaccctcc agatatatcc
      601 ccgtattacc cactatcgcc tggcaccgta ggacaaatcc cccatccgct aggatggtta
      661 gtaccacagc aaggtcaacc agtgtaccca atcacgacag gaggattcag acacccctac
      721 cccacagctc tgaccgtcaa tgcttccatg tccaggttcc ctccccatat ggtcccacca
      781 catcatacgc tacacacgac gggcattccg catccggcca tagtcacacc aacagtcaaa
      841 caggaatcgt cccagagtga tgtcggctca ctccatagtt caaagcatca ggactccaaa
      901 aaggaagaag aaaagaagaa gccccacata aagaaacctc ttaatgcatt catgttgtat
      961 atgaaggaaa tgagagcaaa ggtcgtagct gagtgcacgt tgaaagaaag cgcggccatc
     1021 aaccagatcc ttgggcggag gtggcatgca ctgtccagag aagagcaagc gaaatactac
     1081 gagctggccc ggaaggagcg acagcttcat atgcaactgt accccggctg gtccgcgcgg
     1141 gataactatg gaaagaagaa gaagaggaaa agggacaagc agccgggaga gaccaatgaa
     1201 cacagcgaat gtttcctaaa tccttgcctt tcacttcctc cgattacaga cctgagcgct
     1261 cctaagaaat gccgagcgcg ctttggcctt gatcaacaga ataactggtg cggcccttgc
     1321 aggagaaaaa aaaagtgcgt tcgctacata caaggtgaag gcagctgcct cagcccaccc
     1381 tcttcagatg gaagcttact agattcgcct cccccctccc cgaacctgct aggctcccct
     1441 ccccgagacg ccaagtcaca gactgagcag acccagcctc tgtcgctgtc cctgaagccc
     1501 gaccccctgg cccacctgtc catgatgcct ccgccacccg ccctcctgct cgctgaggcc
     1561 acccacaagg cctccgccct ctgtcccaac ggggccctgg acctgccccc agccgctttg
     1621 cagcctgccg ccccctcctc atcaattgca cagccgtcga cttcttcctt acattcccac
     1681 agctccctgg ccgggaccca gccccagccg ctgtcgctcg tcaccaagtc tttagaatag
     1741 acccagcttt cttgtacaaa gtggtgataa tcgaattcca cggggttggg gttgcgcctt
     1801 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     1861 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     1921 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     1981 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     2041 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     2101 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     2161 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     2221 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     2281 ctctctcccc agggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     2341 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2401 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2461 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2521 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2581 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2641 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2701 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2761 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2821 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2881 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2941 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3001 atcactctcg gcatggacga gctgtacaag gctagcggat caggtcaatg tactaactac
     3061 gctttgttga agcttgccgg cgatgttgaa agtaaccccg gtcctggatc catgtccaga
     3121 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
     3181 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3241 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3301 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3361 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3421 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3481 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3541 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3601 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     3661 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3721 gaaagcggcg ggccggcctc gagcgaattc cagtacctgc cagatacaga cgatcgtcac
     3781 cggattgagg agaaacgtaa aaggacatat gagaccttca agagcatcat gaagaagagt
     3841 cctttcagcg gacccaccga cccccggcct ccacctcgac gcattgctgt gccttcccgc
     3901 agctcagctt ctgtccccaa gccagcaccc cagccctatc cctttacgtc atccctgagc
     3961 accatcaact atgatgagtt tcccaccatg gtgtttcctt ctgggcagat cagccaggcc
     4021 tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc tgcccctgct
     4081 ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct agccccaggc
     4141 cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga aggaacgctg
     4201 tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt gcttggcaac
     4261 agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga gtttcagcag
     4321 ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct gatggagtac
     4381 cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc agctcctgct
     4441 ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga cttctcctcc
     4501 attgcggaca tggacttctc agccctgctg agtcagatca gctcctaaag atctgatcaa
     4561 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4621 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4681 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4741 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4801 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4861 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4921 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4981 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5041 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5101 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5161 tcgataccgt cgacctcgag gttcgcttta agaccaatga cttacaaggc agctgtagat
     5221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5281 caagatggga tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat
     5341 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5521 agtgtggaaa atctctagca gcatctagtt gaatcgccag tgtgtcgagc tagcagatct
     5581 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     5641 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     5701 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     5761 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     5821 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     5881 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     5941 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     6001 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     6241 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6361 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6421 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6541 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     6601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt acggatacct
     6961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8521 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt
     8581 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8641 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8701 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8761 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     8821 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     8881 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8941 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     9001 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9061 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9121 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9181 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9241 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9301 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9361 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     9421 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     9481 acgattcgca gttaatactg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     9541 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     9601 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     9661 caagatagag gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag caggatcttc
     9721 agacctggaa attccctaca atccccaaag tcaaggagta gtagaatcta tgaataaaga
     9781 attaaagaaa attataggac aggtaagaga tcaggctgaa catcttaaga cagcagtaca
     9841 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     9901 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9961 tacaaaaatt caaaattttc gggtttatta cagggacagc agaaatccac tttggaaagg
    10021 accagcaaag ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat
    10081 aaaagtagtg ccaagaagaa aagcaaagat cattagggat tatggaaaac agatggcagg
    10141 tgatgattgt gtggcaagta gacaggatga ggattagaac atggaaaagt ttagtaaaac
    10201 accataagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10981 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    11041 gaacggatct cgacggtatc tagtgattat cggatcaact ttgtatagaa aagttgggta
    11101 caactcccac tagacatatc ctactctatc attgatagag tgacccttcc atagacatat
    11161 cctactctat cattgataga gtgacccttc cactaggttc acttttctct atcattgata
    11221 gggagtggta aacaattgat ggacaaaatc tatcaatgat agagagtgca tgttactagt
    11281 attgttcttc tctatcattg atagtgactt tgcctaatca cttagcaact ctatcattga
    11341 tagagttcta gtattagcaa ggcctttcct ctatataagg aagttcattt catttggaga
    11401 agatccccca agtttgtaca aaaaagcagg cttcaccatg
//
