LOCUS       sequence-218736-       11683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of DVL2 protein with a deletion of its 
            DIX-domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCWXPGR-pTF-DVL2deltaDIX
FEATURES             Location/Qualifiers
     source          1..11683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    379..390
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(1957..1981)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1998..2508
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2427..2445
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2524..2533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3262..3321
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4390..4761
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4787..5375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4840..4860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5258..5269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5378..5394)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5379..5395)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5523..5556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5576..5756
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5794..5813)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5859..5914
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5913..5932)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6275..6291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6275..6291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6323..6329,6330..6347,6348..6353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6368..6389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6448..6643
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6494..6629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6649..6783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6851..6868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7022..7609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7102..7121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7780..8571,8572..8640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8403..8422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8641..8745)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8776..9155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9157..9414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9461..9586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10078..10195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10555..10788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10973..11017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    11292..11312
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11339..11357
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11380..11398
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11544..11562
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11624..11648
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11669..11683
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gttacctggc ttgtgtcgtc agatacccca cagcctgagg tggctccccc agcccatgag
       61 tctcggacag aactggttcc tccgcctcca cccttacccc ctttgccacc agaaaggacc
      121 agtggaattg gggactcaag gcctccatcc ttccacccta atgtatccag cagccatgaa
      181 aatctagagc ctgagacaga gaccgaatct gtcgtatcac tgaggcgaga ccgacctagg
      241 aggagagaca gcagtgaaca tggcgctggt ggccacaggc ctggtggccc ctcgaggctg
      301 gagcgccacc tggctggcta cgagagctct tccaccctca tgaccagtga gctggagagt
      361 accagcctag gagactcgga tgaggatgac accatgagta ggttcagcag ctccactgag
      421 cagagcagtg cctcccgcct cctcaagcgc caccgaaggc gaaggaagca gcggccgcca
      481 cgcatggaga ggacctcatc cttcagcagt gtcaccgatt ccacaatgtc tctcaacatc
      541 atcacggtca cgctcaacat ggagaagtac aacttcctgg gcatctccat tgtgggccaa
      601 agtaacgagc gtggtgatgg gggcatctac atcggctcca tcatgaaagg gggcgcggtg
      661 gctgcggacg ggcggatcga gcctggagac atgcttttgc aggtaaatga tatgaacttt
      721 gagaacatga gcaacgacga tgctgtacga gtgctgagag acatcgtgca caagccaggc
      781 cccatcgtgc tgaccgtggc caagtgttgg gacccgtctc cccaggccta cttcaccctc
      841 cctcgaaatg agcccatcca gcccatcgac ccagccgcct gggtgtcgca ctcggctgcg
      901 ctgactggcg ccttccctgc ttaccctggc tcctcatcta tgagcactat cacatctggc
      961 tcctctctgc ctgatggctg cgaaggccgg ggtctctctg tccacatgga catggcctct
     1021 gtcaccaagg ccatggcagc cccagagtct gggctcgaag tccgggaccg catgtggctc
     1081 aagatcacca tcccaaacgc ctttctaggc tccgatgtgg tggactggct gtaccatcac
     1141 gtggaaggtt ttcctgagcg ccgggaggcc cgcaagtatg ccagtgggct gttgaaggcg
     1201 ggactcatcc ggcacaccgt caacaagatt actttctctg agcagtgcta ttatgtcttc
     1261 ggggacctca gtggtggctg tgagagttac ctagttaacc tctctctgaa tgacaatgac
     1321 ggttccagtg gggcttcaga ccaggatacc ctggcacctc tgcctggagc tactccctgg
     1381 cccctgctgc ccaccttctc ctaccagtat ccagcgccac acccctacag cccccagcct
     1441 ccaccctacc acgagctttc ttcgtacacc tatggtggag gcagtgccag cagccagcac
     1501 agtgagggga gccggagcag cgggtcgaca agaagcgatg ggggggctgg gcgcacaggc
     1561 aggcctgagg aacgggcccc tgagtccaag tccggcagtg gcagtgagtc agaactctcc
     1621 agccggggag gcagccttcg gcggggtggg gagcctggtg ggactggtga tggcggccct
     1681 cctccatcca ggggctcgac aggcgctcct cctaatctcc gagctcttcc cgggctccat
     1741 ccctatggag ccccatctgg catggctctc ccctataacc ccatgatggt agttatgatg
     1801 cctccacccc caccccctgt ctccacagca gtgcagcccc ctggtgcccc tccagtcaga
     1861 gacctgggct ccgtgccccc agaactgaca gctagccgtc agagcttcca catggccatg
     1921 ggcaacccca gtgagttttt tgtggatgtt atgtagaccc agctttcttg tacaaagtgg
     1981 tgataatcga attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     2041 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     2101 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     2161 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     2221 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     2281 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     2341 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     2401 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     2461 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg
     2521 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaaggcta gcggatcagg tcaatgtact aactacgctt tgttgaagct tgccggcgat
     3301 gttgaaagta accccggtcc tggatccatg tccagactgg acaagagcaa agtcataaac
     3361 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     3421 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     3481 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     3541 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
     3601 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     3661 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     3721 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     3781 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     3841 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     3901 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggcctcgagc
     3961 gaattccagt acctgccaga tacagacgat cgtcaccgga ttgaggagaa acgtaaaagg
     4021 acatatgaga ccttcaagag catcatgaag aagagtcctt tcagcggacc caccgacccc
     4081 cggcctccac ctcgacgcat tgctgtgcct tcccgcagct cagcttctgt ccccaagcca
     4141 gcaccccagc cctatccctt tacgtcatcc ctgagcacca tcaactatga tgagtttccc
     4201 accatggtgt ttccttctgg gcagatcagc caggcctcgg ccttggcccc ggcccctccc
     4261 caagtcctgc cccaggctcc agcccctgcc cctgctccag ccatggtatc agctctggcc
     4321 caggccccag cccctgtccc agtcctagcc ccaggccctc ctcaggctgt ggccccacct
     4381 gcccccaagc ccacccaggc tggggaagga acgctgtcag aggccctgct gcagctgcag
     4441 tttgatgatg aagacctggg ggccttgctt ggcaacagca cagacccagc tgtgttcaca
     4501 gacctggcat ccgtcgacaa ctccgagttt cagcagctgc tgaaccaggg catacctgtg
     4561 gccccccaca caactgagcc catgctgatg gagtaccctg aggctataac tcgcctagtg
     4621 acaggggccc agaggccccc cgacccagct cctgctccac tgggggcccc ggggctcccc
     4681 aatggcctcc tttcaggaga tgaagacttc tcctccattg cggacatgga cttctcagcc
     4741 ctgctgagtc agatcagctc ctaaagatct gatcaagctt atcgataatc aacctctgga
     4801 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4861 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4921 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4981 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5041 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5101 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5161 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5221 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5281 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5341 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgaggttc
     5401 gctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5461 gggggactgg aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg
     5521 gaataacttc gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc
     5581 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5641 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5701 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     5761 ctagttgaat cgccagtgtg tcgagctagc agatcttttt ccctctgcca aaaattatgg
     5821 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     5881 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     5941 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     6001 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     6061 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     6121 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     6181 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     6241 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     6481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6781 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7141 gctctcctgt tccgaccctg ccgcttacgg atacctgtcc gcctttctcc cttcgggaag
     7201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7441 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8761 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
     8821 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     8881 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     8941 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     9001 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     9061 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     9121 agtacatcta cgtattagtc atcgctatta ccatgggagg cgtggcctgg gcgggactgg
     9181 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     9241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     9421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     9481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     9541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     9601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     9661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atactggcct
     9721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     9781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     9841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     9901 aagtaagaaa aaagcacagc aagcagcagg atcttcagac ctggaaattc cctacaatcc
     9961 ccaaagtcaa ggagtagtag aatctatgaa taaagaatta aagaaaatta taggacaggt
    10021 aagagatcag gctgaacatc ttaagacagc agtacaaatg gcagtattca tccacaattt
    10081 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    10141 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    10201 ttattacagg gacagcagaa atccactttg gaaaggacca gcaaagctcc tctggaaagg
    10261 tgaaggggca gtagtaatac aagataatag tgacataaaa gtagtgccaa gaagaaaagc
    10321 aaagatcatt agggattatg gaaaacagat ggcaggtgat gattgtgtgg caagtagaca
    10381 ggatgaggat tagaacatgg aaaagtttag taaaacacca taaggaggag atatgaggga
    10441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    11221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatctagt
    11281 gattatcgga tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac
    11341 tctatcattg atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga
    11401 cccttccact aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac
    11461 aaaatctatc aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag
    11521 tgactttgcc taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc
    11581 tttcctctat ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa
    11641 agcaggcttc accatggact acaaagacga tgacgacgac aag
//
