LOCUS       sequence-218738-       10917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ty3-IN-GFP; alpha factor inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Beliakova-Bethell N, Beckham C, Giddings TH Jr, Winey M, Parker R, 
            Sandmeyer S
  TITLE     Virus-like particles of the Ty3 retrotransposon assemble in 
            association with P-body components.
  JOURNAL   RNA. 2006 Jan;12(1):94-101. doi: 10.1261/rna.2264806.
  PUBMED    16373495
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1261/rna"; 
            journalName: "RNA"; date: "2006-01"; volume: "12"; issue: "1"; 
            pages: "94-101"
COMMENT     Sequence Label: pNB2176
FEATURES             Location/Qualifiers
     source          1..10917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2802..2818
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2803..2819
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7888..8601
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7918..7946)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8541..8561
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8936..8955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8937..8955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8962..8979)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8962..8978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8971..8993)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9119..9574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9206..9225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9416..9437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9705..10508)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10509..10724)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10748..10767)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10867..10889
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2761 accctcacta aagggaacaa aagctggtac cgggcccccc ctcgaggtcg acggtatcga
     2821 taagctttgt tgtatctcaa aatgagatat gtcagtatga caatacgtca ccctgaacgt
     2881 tcataaaaca catatgaaac aaccttataa caaaacgaac aacatgagac aaaacccgac
     2941 cttccctagc tgaactaccc aaagtataaa tgcctgaaca attagtttag atccgagatt
     3001 ccgcgcttcc accacttagt atgattcata ttttatataa tatataagat aagtaacatt
     3061 ccgtgaatta atctgataaa ctgttttgac aactggttac ttccctaaga ctgtttatat
     3121 taggattgtc aagacactcc ggtattactc gagcccgtaa tacaacacct ggtagcgtta
     3181 aaggttacta attgttcaaa cgaaccatcg aaaagccgaa cctagctaca ccacacccca
     3241 gtatgagctt tatggatcaa atcccaggag gaggaaatta tccaaaactc ccagtagaat
     3301 gccttcctaa cttcccgatc caaccatctt tgaccttcag aggtagaaat gactcgcata
     3361 aactgaaaaa ctttatctcc gaaataatgt taaacatgtc tatgatatct tggccgaatg
     3421 atgccagtcg tattgtgtac tgcagaagac atttattaaa ccccgctgct cagtgggcta
     3481 atgactttgt acaagaacaa ggtatacttg aaataacatt cgacacattc atacaaggat
     3541 tatatcagca tttctataag ccaccagata tcaataaaat ctttaatgca atcacgcaac
     3601 tttccgaagc taaacttggt attgagcgtc tcaaccaacg attcagaaag atttgggaca
     3661 gaatgccacc agacttcatg accgaaaaag ctgccataat gacatatact aggctattga
     3721 caaaggaaac ctataatatt gtcagaatgc acaaaccaga gacattaaaa gacgccatgg
     3781 aagaggctta ccagacaact gcactaactg aaagattctt cccaggattc gaacttgatg
     3841 ctgatggaga cactatcatc ggtgccacaa cccacttaca agaagaatac gactctgact
     3901 atgattcaga agataatctg acccagaatg gatacgtcca taccgtaagg acaagaagat
     3961 cttacaataa accaatgtca aatcatcgaa acaggagaaa taacaaccca tctagagaag
     4021 aatgtataaa aaatcggcta tgcttctatt gtaagaaaga gggacatcgc ctgaacgaat
     4081 gtagagcacg taaggcgagt tctaaccgat cttgaactcg aatcaaaaga ccaacaaact
     4141 ccttttatca aaaccttacc aattgtacac tatatcgcca tccccgagat ggacaatacc
     4201 gccgaaaaaa ccataaaaat acaaaacacg aaagtaaaaa ccctgtttga cagtggatca
     4261 cccacgtcat ttatccgaag agatattgta gaacttctca aatacgaaat ctacgagacc
     4321 cctccactcc gttttagagg attcgtagcc accaaatccg ccgttacatc cgaagcagtc
     4381 accattgacc tcaaaatcaa tgacctgcat ataactttag ccgcgtacat actggataac
     4441 atggactacc aattgttaat tggaaatcca atcttacgcc gctacccgaa aatcctgcac
     4501 acagtactga ataccagaga gagccccgac tccttaaagc ccaagactta tcgctccgaa
     4561 accgttaata acgttagaac ctactccgct ggtaatcgtg gtaaccccag aaacataaaa
     4621 ctgtcttttg cccccaccat tctcgaagca actgacccga aatccgctgg taatcgtggt
     4681 gactccagaa ccaaaaccct gtctcttgca accactactc ctgcagcaat tgacccgctt
     4741 acgacccttg ataacccagg tagtactcaa agtacatttg cgcaattccc gatacctgaa
     4801 gaagcgagca tcctagaaga ggatggaaaa tactccaacg ttgtctcaac cattcagagt
     4861 gtagaaccta atgctactga tcacagcaat aaggacacct tttgcacttt gccagtttgg
     4921 ttacaacaga agtatagaga gatcatacgt aatgatctcc caccaagacc tgccgacatt
     4981 aataacatcc ccgtaaaaca tgatattgaa attaaacctg gcgcaagact acctcgacta
     5041 cagccatacc atgttacaga aaagaacgaa caagaaatca acaaaatagt tcaaaaactg
     5101 ctcgataaca agttcattgt tccctcaaag tcgccttgca gctcccctgt agtcctcgtc
     5161 ccgaagaaag acggtacctt ccgactctgc gtcgattacc gcaccctgaa caaagctacc
     5221 atctccgacc cattcccatt acccagaatc gacaacctat tgagccgtat tggaaatgcc
     5281 cagatattta ccacgctaga tttgcatagt ggttaccacc agatcccgat ggaacccaaa
     5341 gaccgctaca aaaccgcctt tgtcacacca tccggtaagt atgaatatac cgtcatgcca
     5401 tttggcttag tcaatgcacc tagtacattc gcaagataca tggctgatac atttagagac
     5461 ctgagattcg tcaatgttta ccttgatgat atattaatat tctccgaatc tccagaagaa
     5521 cattggaaac atttagacac ggtactagaa agattaaaga acgagaacct cattgttaag
     5581 aagaaaaaat gtaaatttgc atctgaagaa actgagtttt taggctatag tattggaatc
     5641 cagaaaatag ctccactaca gcacaaatgt gcagcaatcc gagactttcc gacgcctaaa
     5701 acagtaaaac aagcacagag atttttagga atgattaatt actacagacg attcattcca
     5761 aattgctcca agattgcaca gccaatccaa ctgtttattt gtgacaaaag tcaatggaca
     5821 gaaaaacaag acaaggcaat tgataaacta aaagacgcct tgtgtaactc ccccgtccta
     5881 gtaccattca acaacaaagc aaactaccga cttacaacag acgcctcaaa agacggcatt
     5941 ggtgctgttc tagaagaagt cgacaacaag aacaaacttg ttggtgtcgt cggttacttc
     6001 tctaaatcct tagagagtgc ccagaaaaac tatcctgctg gcgaattaga actacttgga
     6061 attatcaaag cactccacca cttccgatat atgcttcacg gaaagcattt cacgttaaga
     6121 acagaccaca ttagtttgtt atcattacaa aacaagaacg aacccgcacg acgcgtgcaa
     6181 cgctggttag atgacctagc cacatatgac ttcaccttag aatacctagc tggacccaag
     6241 aacgttgtcg cagatgccat atcccgtgcc gtatatacta taacccccga aacatcccga
     6301 cctatcgaca cagaaagctg gaaatcttac tacaaatcag acccattatg tagtgctgtc
     6361 ttaattcata tgaaagaatt gacacaacac aacgtcacac ctgaagatat gtcagccttc
     6421 cgtagttacc agaagaaact cgaactatca gagaccttcc gaaagaatta ttccctagaa
     6481 gacgaaatga tctattacca agaccgacta gtagtaccaa taaaacaaca gaacgcagtt
     6541 atgagactat atcatgacca taccttattt ggaggacatt ttggtgtaac agtgaccctt
     6601 gcgaaaatca gcccaattta ctattggcca aaattacaac attcgatcat acaatacatc
     6661 aggacctgcg tacaatgtca actaataaaa tcacaccgac cacgcttaca tggactatta
     6721 caaccactcc ctatagcaga aggaagatgg cttgatatat caatggattt tgtgacagga
     6781 ttacccccga catcaaataa cttgaatatg atcctcgtcg tagttgatcg tttttcgaaa
     6841 cgcgctcact tcatagctac aaggaaaacc ttagacgcaa cacaactaat agatctactc
     6901 tttcgataca ttttttcata tcatggtttt cccaggacaa taaccagtga tagagatgtc
     6961 cgtatgaccg ccgacaaata tcaagaactc acgaaaagac taggaataaa atcgacaatg
     7021 tcttccgcga accaccccca aacagatgga caatccgaac gaacgataca gacattaaac
     7081 aggttactaa gagcctatgc ttcaaccaat attcagaatt ggcatgtata tttaccacaa
     7141 atcgaatttg tttacaattc tacacctact agaacacttg gaaaatcacc atttgaaatt
     7201 gatttaggat atttaccgaa tacccctgct attaagtcag atgacgaagt caacgcaaga
     7261 agttttactg ccgtagaact tgccaaacac ctcaaagccc ttaccatcca aacgaaggaa
     7321 cagctagaac acgctcaaat cgaaatggaa actaataaca atcaaagacg taaaccctta
     7381 ttgttaaaca taggagatca cgtattagtg catagagatg catacttcaa gaaaggtgct
     7441 tatatgaaag tacaacaaat atacgtcgga ccatttcgag ttgtcaagaa aataaacgat
     7501 aacgcctacg aactagattt aaactctcac aagaaaaagc acagagttat taatgtacaa
     7561 ttcctgaaaa agtttgtata ccgtccagac gcgtacccaa agaataaacc aatcagctcc
     7621 actgaaagaa ttaagagagc acacgaagtt actgcactca taggaataga tactacacac
     7681 aaaacttact tatgtcacat gcaagatgta gacccaacac tttcagtaga atactcagaa
     7741 gctgaatttt gccaaattcc cgaaagaaca cgaagatcaa tattagccaa ctttagacaa
     7801 ctctacgaaa cacaagacaa ccctgagaga gaggaagatg ttgtatctca aaatgagata
     7861 tgtcagtatg acaatacgtc acccgggatg agtaaaggag aagaactttt cactggagtt
     7921 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     7981 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     8041 aaactacctg ttccatggcc aacacttgtc actactttca cttatggtgt tcaatgcttt
     8101 tcaagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
     8161 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     8221 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     8281 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     8341 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     8401 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     8461 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     8521 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     8581 ggcatggatg aactatacaa ataataaccc gggacgttca taaaacacat atgaaacaac
     8641 cttataacaa aacgaacaac atgagacaaa acccgacctt ccctagctga actacccaaa
     8701 gtataaatgc ctgaacaatt agtttagatc cgagattccg cgcttccacc acttagtatg
     8761 attcatattt tatataatat ataagataag taacattccg tgaattaatc tgataaactg
     8821 ttttgacaac tggttacttc cctaagactg tttatattag gattgtcaag acactccggt
     8881 attactcgag cccgtaatac aacagcggcc gccaccgcgg tggagctcca attcgcccta
     8941 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     9001 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     9061 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     9121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9541 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     9601 atctgtgcgg tatttcacac cgcatagggt aataactgat ataattaaat tgaagctcta
     9661 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     9721 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     9781 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     9841 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     9901 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     9961 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
    10021 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
    10081 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
    10141 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
    10201 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
    10261 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
    10321 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
    10381 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
    10441 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
    10501 ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat
    10561 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
    10621 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
    10681 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaaggtg gtatggtgca
    10741 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
    10801 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    10861 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
