LOCUS       sequence-218739-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression of the mCherry living color.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, 
            Zgraggen E, Salmon P, Kiss JZ
  TITLE     Wnt signaling regulates multipolar-to-bipolar transition of 
            migrating neurons in the cerebral cortex.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 
            10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26.
  PUBMED    25732825
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.061"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1349-61"
COMMENT     Sequence Label: pCLX-UBI-mCherry
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1219..1243
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1245..1254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1251..1961
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1396..1414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1664..1683
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1963..1987)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2005..2593
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2058..2078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2476..2487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2736..2769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2789..2969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3007..3026)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3072..3127
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3126..3145)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3488..3504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3488..3504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3501..3523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3512..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3536..3542,3543..3560,3561..3566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3581..3602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3661..3856
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3707..3842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3862..3996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3899..3918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3953..3972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4064..4081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4235..4823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4315..4334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4994..5785,5786..5854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5617..5636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5855..5959)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5990..6369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             6371..6628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6675..6800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7292..7409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7769..8002
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8187..8231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    8506..8526
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acaggatccc aactttgtac aaaaaagcag gctcgccacc atggtgagca
     1261 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1321 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1381 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1441 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1501 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1561 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1621 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1681 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1741 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1801 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1861 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1921 gccgccactc caccggcggc atggacgagc tgtacaagta agacccagct ttcttgtaca
     1981 aagtggtgat aatcgaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     2401 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2521 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2581 gccgcctccc cgcatcggga attcccgcgg ttcgctttaa gaccaatgac ttacaaggca
     2641 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2701 caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc atacattata
     2761 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     2821 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2881 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2941 cttttagtca gtgtggaaaa tctctagcag catctagttg aatcgccagt gtgtcgagct
     3001 agcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     3061 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3121 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     3181 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     3241 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     3301 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     3361 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     3421 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     3481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3541 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     3661 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3721 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3781 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3841 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     3901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3961 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     4021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     6001 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6061 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6361 attaccatgg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     6421 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6481 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6541 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6601 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6661 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6721 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     6781 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     6841 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     6901 gggagctaga acgattcgca gttaatactg gcctgttaga aacatcagaa ggctgtagac
     6961 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7021 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7081 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag
     7141 caggatcttc agacctggaa attccctaca atccccaaag tcaaggagta gtagaatcta
     7201 tgaataaaga attaaagaaa attataggac aggtaagaga tcaggctgaa catcttaaga
     7261 cagcagtaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     7321 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     7381 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agaaatccac
     7441 tttggaaagg accagcaaag ctcctctgga aaggtgaagg ggcagtagta atacaagata
     7501 atagtgacat aaaagtagtg ccaagaagaa aagcaaagat cattagggat tatggaaaac
     7561 agatggcagg tgatgattgt gtggcaagta gacaggatga ggattagaac atggaaaagt
     7621 ttagtaaaac accataagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7681 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7741 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7801 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7861 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7921 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7981 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8041 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8101 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8161 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8221 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8281 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8341 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8401 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8461 ttcgattagt gaacggatct cgacggtatc tagtgattat cggatcaact ttgtatagaa
     8521 aagttgttaa ttaagggtgc agc
//
